Circ_0001602 aggravates the progression of acute myeloid leukemia by regulating the miR-192-5p/ZBTB20 axis
ABSTRACTBackground Acute myeloid leukemia (AML) is a malignant blood cancer with a poor prognosis and complex pathogenesis. Recently, the critical role of circular RNAs (circRNAs) has been demonstrated in the malignant progression of AML. This study aimed to investigate the functional role and under...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2023-12-01
|
| Series: | Hematology |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/16078454.2023.2240133 |
| _version_ | 1827868289406074880 |
|---|---|
| author | Weihao Wu Jiayi Deng Congjie Chen Xiaomei Ma Lian Yu Longtian Chen |
| author_facet | Weihao Wu Jiayi Deng Congjie Chen Xiaomei Ma Lian Yu Longtian Chen |
| author_sort | Weihao Wu |
| collection | DOAJ |
| description | ABSTRACTBackground Acute myeloid leukemia (AML) is a malignant blood cancer with a poor prognosis and complex pathogenesis. Recently, the critical role of circular RNAs (circRNAs) has been demonstrated in the malignant progression of AML. This study aimed to investigate the functional role and underlying mechanism of circ_0001602 in AML development.Methods Quantitative real-time polymerase chain reaction (qRT-PCR) assay was conducted for detecting the expression of circ_0001602, CCND3, microRNA-192-5p (miR-192-5p), and Zinc Finger and BTB Domain-Containing Protein 20 (ZBTB20) mRNA. RNase R assay and Actinomycin D assay were implemented to determine the characteristics of circ_0001602. Cell counting Kit-8 (CCK-8) assay was performed to evaluate cell proliferation. Flow cytometry was employed for assessing cell cycle distribution and apoptosis. Dual-luciferase reporter assay and RIP assay were utilized for confirming the interactions between miR-192-5p and circ_0001602 or ZBTB20.Results Circ_0001602 and ZBTB20 were upregulated and miR-192-5p level was reduced in AML tissues and cells. Depletion of circ_0001602 repressed cell proliferation and induced cell cycle arrest and apoptosis in AML cells. Functionally, circ_0001602 was identified to be the sponge of miR-192-5p, and miR-192-5p silence restored the suppressive effects of circ_0001602 knockdown on AML cell progression. Furthermore, ZBTB20 was a target of miR-192-5p, and ZBTB20 overexpression neutralized the miR-192-5p-mediated inhibiting actions on the malignant phenotypes of AML cells. Besides, circ_0001602 could sponge miR-192-5p to positively regulate ZBTB20 expression.Conclusion Circ_0001602 contributed to AML cell development at least partially through modulating the miR-192-5p/ZBTB20 axis, which provided new insights for AML treatment. |
| first_indexed | 2024-03-12T15:28:33Z |
| format | Article |
| id | doaj.art-6bf445bb13aa4e89ae08e7e51ab29a0d |
| institution | Directory Open Access Journal |
| issn | 1607-8454 |
| language | English |
| last_indexed | 2024-03-12T15:28:33Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Hematology |
| spelling | doaj.art-6bf445bb13aa4e89ae08e7e51ab29a0d2023-08-10T10:28:31ZengTaylor & Francis GroupHematology1607-84542023-12-0128110.1080/16078454.2023.2240133Circ_0001602 aggravates the progression of acute myeloid leukemia by regulating the miR-192-5p/ZBTB20 axisWeihao Wu0Jiayi Deng1Congjie Chen2Xiaomei Ma3Lian Yu4Longtian Chen5Department of Hematology, Longyan First Affiliated Hospital of Fujian Medical University, Longyan City, People’s Republic of ChinaDepartment of Hematology, Longyan First Affiliated Hospital of Fujian Medical University, Longyan City, People’s Republic of ChinaDepartment of Hematology, Longyan First Affiliated Hospital of Fujian Medical University, Longyan City, People’s Republic of ChinaDepartment of Hematology, Longyan First Affiliated Hospital of Fujian Medical University, Longyan City, People’s Republic of ChinaDepartment of Hematology, Longyan First Affiliated Hospital of Fujian Medical University, Longyan City, People’s Republic of ChinaDepartment of Hematology, Longyan First Affiliated Hospital of Fujian Medical University, Longyan City, People’s Republic of ChinaABSTRACTBackground Acute myeloid leukemia (AML) is a malignant blood cancer with a poor prognosis and complex pathogenesis. Recently, the critical role of circular RNAs (circRNAs) has been demonstrated in the malignant progression of AML. This study aimed to investigate the functional role and underlying mechanism of circ_0001602 in AML development.Methods Quantitative real-time polymerase chain reaction (qRT-PCR) assay was conducted for detecting the expression of circ_0001602, CCND3, microRNA-192-5p (miR-192-5p), and Zinc Finger and BTB Domain-Containing Protein 20 (ZBTB20) mRNA. RNase R assay and Actinomycin D assay were implemented to determine the characteristics of circ_0001602. Cell counting Kit-8 (CCK-8) assay was performed to evaluate cell proliferation. Flow cytometry was employed for assessing cell cycle distribution and apoptosis. Dual-luciferase reporter assay and RIP assay were utilized for confirming the interactions between miR-192-5p and circ_0001602 or ZBTB20.Results Circ_0001602 and ZBTB20 were upregulated and miR-192-5p level was reduced in AML tissues and cells. Depletion of circ_0001602 repressed cell proliferation and induced cell cycle arrest and apoptosis in AML cells. Functionally, circ_0001602 was identified to be the sponge of miR-192-5p, and miR-192-5p silence restored the suppressive effects of circ_0001602 knockdown on AML cell progression. Furthermore, ZBTB20 was a target of miR-192-5p, and ZBTB20 overexpression neutralized the miR-192-5p-mediated inhibiting actions on the malignant phenotypes of AML cells. Besides, circ_0001602 could sponge miR-192-5p to positively regulate ZBTB20 expression.Conclusion Circ_0001602 contributed to AML cell development at least partially through modulating the miR-192-5p/ZBTB20 axis, which provided new insights for AML treatment.https://www.tandfonline.com/doi/10.1080/16078454.2023.2240133AMLcirc_0001602miR-192-5pZBTB20 |
| spellingShingle | Weihao Wu Jiayi Deng Congjie Chen Xiaomei Ma Lian Yu Longtian Chen Circ_0001602 aggravates the progression of acute myeloid leukemia by regulating the miR-192-5p/ZBTB20 axis Hematology AML circ_0001602 miR-192-5p ZBTB20 |
| title | Circ_0001602 aggravates the progression of acute myeloid leukemia by regulating the miR-192-5p/ZBTB20 axis |
| title_full | Circ_0001602 aggravates the progression of acute myeloid leukemia by regulating the miR-192-5p/ZBTB20 axis |
| title_fullStr | Circ_0001602 aggravates the progression of acute myeloid leukemia by regulating the miR-192-5p/ZBTB20 axis |
| title_full_unstemmed | Circ_0001602 aggravates the progression of acute myeloid leukemia by regulating the miR-192-5p/ZBTB20 axis |
| title_short | Circ_0001602 aggravates the progression of acute myeloid leukemia by regulating the miR-192-5p/ZBTB20 axis |
| title_sort | circ 0001602 aggravates the progression of acute myeloid leukemia by regulating the mir 192 5p zbtb20 axis |
| topic | AML circ_0001602 miR-192-5p ZBTB20 |
| url | https://www.tandfonline.com/doi/10.1080/16078454.2023.2240133 |
| work_keys_str_mv | AT weihaowu circ0001602aggravatestheprogressionofacutemyeloidleukemiabyregulatingthemir1925pzbtb20axis AT jiayideng circ0001602aggravatestheprogressionofacutemyeloidleukemiabyregulatingthemir1925pzbtb20axis AT congjiechen circ0001602aggravatestheprogressionofacutemyeloidleukemiabyregulatingthemir1925pzbtb20axis AT xiaomeima circ0001602aggravatestheprogressionofacutemyeloidleukemiabyregulatingthemir1925pzbtb20axis AT lianyu circ0001602aggravatestheprogressionofacutemyeloidleukemiabyregulatingthemir1925pzbtb20axis AT longtianchen circ0001602aggravatestheprogressionofacutemyeloidleukemiabyregulatingthemir1925pzbtb20axis |