The boundary of life and death: changes in mitochondrial and cytosolic proteomes associated with programmed cell death of Arabidopsis thaliana suspension culture cells
IntroductionDespite the critical role of programmed cell death (PCD) in plant development and defense responses, its regulation is not fully understood. It has been proposed that mitochondria may be important in the control of the early stages of plant PCD, but the details of this regulation are cur...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-08-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1194866/full |
| _version_ | 1797767074308685824 |
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| author | Johanna Schwarze James C. Carolan Gavin S. Stewart Paul F. McCabe Joanna Kacprzyk |
| author_facet | Johanna Schwarze James C. Carolan Gavin S. Stewart Paul F. McCabe Joanna Kacprzyk |
| author_sort | Johanna Schwarze |
| collection | DOAJ |
| description | IntroductionDespite the critical role of programmed cell death (PCD) in plant development and defense responses, its regulation is not fully understood. It has been proposed that mitochondria may be important in the control of the early stages of plant PCD, but the details of this regulation are currently unknown. MethodsWe used Arabidopsis thaliana cell suspension culture, a model system that enables induction and precise monitoring of PCD rates, as well as chemical manipulation of this process to generate a quantitative profile of the alterations in mitochondrial and cytosolic proteomes associated with early stages of plant PCD induced by heat stress. The cells were subjected to PCD-inducing heat levels (10 min, 54°C), with/without the calcium channel inhibitor and PCD blocker LaCl3. The stress treatment was followed by separation of cytosolic and mitochondrial fractions and mass spectrometry-based proteome analysis. ResultsHeat stress induced rapid and extensive changes in protein abundance in both fractions, with release of mitochondrial proteins into the cytosol upon PCD induction. In our system, LaCl3 appeared to act downstream of cell death initiation signal, as it did not affect the release of mitochondrial proteins, but instead partially inhibited changes occurring in the cytosolic fraction, including upregulation of proteins with hydrolytic activity. DiscussionWe characterized changes in protein abundance and localization associated with the early stages of heat stress-induced PCD. Collectively, the generated data provide new insights into the regulation of cell death and survival decisions in plant cells. |
| first_indexed | 2024-03-12T20:34:16Z |
| format | Article |
| id | doaj.art-6bf7d550f2ef473c966f61aeab7399da |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-03-12T20:34:16Z |
| publishDate | 2023-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-6bf7d550f2ef473c966f61aeab7399da2023-08-01T15:48:47ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-08-011410.3389/fpls.2023.11948661194866The boundary of life and death: changes in mitochondrial and cytosolic proteomes associated with programmed cell death of Arabidopsis thaliana suspension culture cellsJohanna Schwarze0James C. Carolan1Gavin S. Stewart2Paul F. McCabe3Joanna Kacprzyk4School of Biology and Environmental Science, University College Dublin, Dublin, IrelandDepartment of Biology, Maynooth University, Maynooth, IrelandSchool of Biology and Environmental Science, University College Dublin, Dublin, IrelandSchool of Biology and Environmental Science, University College Dublin, Dublin, IrelandSchool of Biology and Environmental Science, University College Dublin, Dublin, IrelandIntroductionDespite the critical role of programmed cell death (PCD) in plant development and defense responses, its regulation is not fully understood. It has been proposed that mitochondria may be important in the control of the early stages of plant PCD, but the details of this regulation are currently unknown. MethodsWe used Arabidopsis thaliana cell suspension culture, a model system that enables induction and precise monitoring of PCD rates, as well as chemical manipulation of this process to generate a quantitative profile of the alterations in mitochondrial and cytosolic proteomes associated with early stages of plant PCD induced by heat stress. The cells were subjected to PCD-inducing heat levels (10 min, 54°C), with/without the calcium channel inhibitor and PCD blocker LaCl3. The stress treatment was followed by separation of cytosolic and mitochondrial fractions and mass spectrometry-based proteome analysis. ResultsHeat stress induced rapid and extensive changes in protein abundance in both fractions, with release of mitochondrial proteins into the cytosol upon PCD induction. In our system, LaCl3 appeared to act downstream of cell death initiation signal, as it did not affect the release of mitochondrial proteins, but instead partially inhibited changes occurring in the cytosolic fraction, including upregulation of proteins with hydrolytic activity. DiscussionWe characterized changes in protein abundance and localization associated with the early stages of heat stress-induced PCD. Collectively, the generated data provide new insights into the regulation of cell death and survival decisions in plant cells. https://www.frontiersin.org/articles/10.3389/fpls.2023.1194866/fullplant programmed cell deathmitochondriaproteomicsheat stresscytosol |
| spellingShingle | Johanna Schwarze James C. Carolan Gavin S. Stewart Paul F. McCabe Joanna Kacprzyk The boundary of life and death: changes in mitochondrial and cytosolic proteomes associated with programmed cell death of Arabidopsis thaliana suspension culture cells Frontiers in Plant Science plant programmed cell death mitochondria proteomics heat stress cytosol |
| title | The boundary of life and death: changes in mitochondrial and cytosolic proteomes associated with programmed cell death of Arabidopsis thaliana suspension culture cells |
| title_full | The boundary of life and death: changes in mitochondrial and cytosolic proteomes associated with programmed cell death of Arabidopsis thaliana suspension culture cells |
| title_fullStr | The boundary of life and death: changes in mitochondrial and cytosolic proteomes associated with programmed cell death of Arabidopsis thaliana suspension culture cells |
| title_full_unstemmed | The boundary of life and death: changes in mitochondrial and cytosolic proteomes associated with programmed cell death of Arabidopsis thaliana suspension culture cells |
| title_short | The boundary of life and death: changes in mitochondrial and cytosolic proteomes associated with programmed cell death of Arabidopsis thaliana suspension culture cells |
| title_sort | boundary of life and death changes in mitochondrial and cytosolic proteomes associated with programmed cell death of arabidopsis thaliana suspension culture cells |
| topic | plant programmed cell death mitochondria proteomics heat stress cytosol |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1194866/full |
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