Chamber‐based system for measuring whole‐plant transpiration dynamics
Abstract Most of our insights on whole‐plant transpiration (E) are based on leaf‐chamber measurements using water vapor porometers, IRGAs, or flux measurements. Gravimetric methods are integrative, accurate, and a clear differentiation between evaporation and E can be made. Water vapor pressure defi...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-12-01
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| Series: | Plant-Environment Interactions |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/pei3.10094 |
| _version_ | 1797974765250543616 |
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| author | Alejandro Pieters Marcus Giese Marc Schmierer Kristian Johnson Folkard Asch |
| author_facet | Alejandro Pieters Marcus Giese Marc Schmierer Kristian Johnson Folkard Asch |
| author_sort | Alejandro Pieters |
| collection | DOAJ |
| description | Abstract Most of our insights on whole‐plant transpiration (E) are based on leaf‐chamber measurements using water vapor porometers, IRGAs, or flux measurements. Gravimetric methods are integrative, accurate, and a clear differentiation between evaporation and E can be made. Water vapor pressure deficit (VPD) is the driving force for E but assessing its impact has been evasive, due to confounding effects of other climate drivers. We developed a chamber‐based gravimetric method, in which whole plant response of E to VPD could be assessed, while keeping other environmental parameters at predetermined values. Stable VPD values (0.5–3.7 kPa) were attained within 5 min after changing flow settings and maintained for at least 45 min. Species differing in life form and photosynthetic metabolism were used. Typical runs covering the range of VPDs lasted up to 4 h, preventing acclimation responses or soilborne water deficit. Species‐specific responses of E to VPD could be identified, as well as differences in leaf conductance. The combined gravimetric‐chamber‐based system presented overcomes several limitations of previous gravimetric set ups in terms of replicability, time, and elucidation of the impact of specific environmental drivers on E, filling a methodological gap and widening our phenotyping capabilities. |
| first_indexed | 2024-04-11T04:24:56Z |
| format | Article |
| id | doaj.art-6bfe8a92908b4063922a0fbd78ef5426 |
| institution | Directory Open Access Journal |
| issn | 2575-6265 |
| language | English |
| last_indexed | 2024-04-11T04:24:56Z |
| publishDate | 2022-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Plant-Environment Interactions |
| spelling | doaj.art-6bfe8a92908b4063922a0fbd78ef54262022-12-30T02:02:40ZengWileyPlant-Environment Interactions2575-62652022-12-013624325310.1002/pei3.10094Chamber‐based system for measuring whole‐plant transpiration dynamicsAlejandro Pieters0Marcus Giese1Marc Schmierer2Kristian Johnson3Folkard Asch4Institute for Agricultural Sciences in the Tropics (Hans‐Ruthenberg Institute) University of Hohenheim Stuttgart GermanyInstitute for Agricultural Sciences in the Tropics (Hans‐Ruthenberg Institute) University of Hohenheim Stuttgart GermanyInstitute for Agricultural Sciences in the Tropics (Hans‐Ruthenberg Institute) University of Hohenheim Stuttgart GermanyInstitute for Agricultural Sciences in the Tropics (Hans‐Ruthenberg Institute) University of Hohenheim Stuttgart GermanyInstitute for Agricultural Sciences in the Tropics (Hans‐Ruthenberg Institute) University of Hohenheim Stuttgart GermanyAbstract Most of our insights on whole‐plant transpiration (E) are based on leaf‐chamber measurements using water vapor porometers, IRGAs, or flux measurements. Gravimetric methods are integrative, accurate, and a clear differentiation between evaporation and E can be made. Water vapor pressure deficit (VPD) is the driving force for E but assessing its impact has been evasive, due to confounding effects of other climate drivers. We developed a chamber‐based gravimetric method, in which whole plant response of E to VPD could be assessed, while keeping other environmental parameters at predetermined values. Stable VPD values (0.5–3.7 kPa) were attained within 5 min after changing flow settings and maintained for at least 45 min. Species differing in life form and photosynthetic metabolism were used. Typical runs covering the range of VPDs lasted up to 4 h, preventing acclimation responses or soilborne water deficit. Species‐specific responses of E to VPD could be identified, as well as differences in leaf conductance. The combined gravimetric‐chamber‐based system presented overcomes several limitations of previous gravimetric set ups in terms of replicability, time, and elucidation of the impact of specific environmental drivers on E, filling a methodological gap and widening our phenotyping capabilities.https://doi.org/10.1002/pei3.10094environmental driversgravimetrytranspirationVPDwhole plant |
| spellingShingle | Alejandro Pieters Marcus Giese Marc Schmierer Kristian Johnson Folkard Asch Chamber‐based system for measuring whole‐plant transpiration dynamics Plant-Environment Interactions environmental drivers gravimetry transpiration VPD whole plant |
| title | Chamber‐based system for measuring whole‐plant transpiration dynamics |
| title_full | Chamber‐based system for measuring whole‐plant transpiration dynamics |
| title_fullStr | Chamber‐based system for measuring whole‐plant transpiration dynamics |
| title_full_unstemmed | Chamber‐based system for measuring whole‐plant transpiration dynamics |
| title_short | Chamber‐based system for measuring whole‐plant transpiration dynamics |
| title_sort | chamber based system for measuring whole plant transpiration dynamics |
| topic | environmental drivers gravimetry transpiration VPD whole plant |
| url | https://doi.org/10.1002/pei3.10094 |
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