Effects of gene polymorphisms of CYP1A1, CYP1B1, EPHX1, NQO1, and NAT2 on urinary 1-nitropyrene metabolite concentrations
Nitropyrene (1-NP) is a specific indicator of exposure to diesel exhaust and is partly metabolized to 1-aminopyrene (1-AP) and N-acetyl-1-aminopyrene (1-NAAP), which are excreted in urine. This study was conducted to evaluate the effects of gene polymorphisms of metabolic enzymes for 1-NP on the uri...
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| Language: | English |
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Elsevier
2022-08-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844022014086 |
| _version_ | 1811337890489171968 |
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| author | Jung-Kuk Yun Bolormaa Ochirpurev Sang-Yong Eom Akira Toriba Yong-Dae Kim Heon Kim |
| author_facet | Jung-Kuk Yun Bolormaa Ochirpurev Sang-Yong Eom Akira Toriba Yong-Dae Kim Heon Kim |
| author_sort | Jung-Kuk Yun |
| collection | DOAJ |
| description | Nitropyrene (1-NP) is a specific indicator of exposure to diesel exhaust and is partly metabolized to 1-aminopyrene (1-AP) and N-acetyl-1-aminopyrene (1-NAAP), which are excreted in urine. This study was conducted to evaluate the effects of gene polymorphisms of metabolic enzymes for 1-NP on the urinary concentrations of 1-AP and 1-NAAP. The study participants were 70 South Koreans who were occupationally or environmentally exposed to diesel exhaust. To evaluate 1-NP exposure levels, we sampled airborne particulate matters with a personal air sampler and measured urinary 1-AP and 1-NAAP concentrations. The genetic polymorphisms of the 1-NP metabolic enzymes (CYP1A1, CYP1B1, EPHX1, NQO1, and NAT2) were determined by direct sequencing. The mean 1-NP exposure level was 20.40 pg/m3, and the mean urinary concentrations of 1-AP and 1-NAAP were 0.074 nM and 0.213 nM, respectively. The correlation coefficient between the 1-NP exposure level and urinary 1-AP concentrations was 0.0138 and that between the 1-NP exposure level and urinary 1-NAAP concentrations was 0.1493, and neither correlation coefficient was statistically significant. The correlation coefficient between the 1-NP exposure level and urinary 1-AP concentrations showed statistically significant differences according to the CYP1A1 and CYP1B1 genotypes, and that between the 1-NP exposure level and urinary 1-NAAP concentrations was significantly different according to the CYP1A1, CYP1B1, and NAT2 genotypes. The urinary concentration of 1-NAAP is a better biomarker for exposure to 1-NP or DEPs because the former is higher, easier to measure, and more strongly correlated with 1-NP exposure levels than that of 1-AP. The relationship between 1-NP exposure and urinary 1-AP or 1-NAAP concentration depends on the single nucleotide polymorphism types of CYP1A1, CYP1B1, NQO1, and NAT2. |
| first_indexed | 2024-04-13T18:01:42Z |
| format | Article |
| id | doaj.art-6c05f9c92721451895da93b628308653 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-13T18:01:42Z |
| publishDate | 2022-08-01 |
| publisher | Elsevier |
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| series | Heliyon |
| spelling | doaj.art-6c05f9c92721451895da93b6283086532022-12-22T02:36:13ZengElsevierHeliyon2405-84402022-08-0188e10120Effects of gene polymorphisms of CYP1A1, CYP1B1, EPHX1, NQO1, and NAT2 on urinary 1-nitropyrene metabolite concentrationsJung-Kuk Yun0Bolormaa Ochirpurev1Sang-Yong Eom2Akira Toriba3Yong-Dae Kim4Heon Kim5Avellino Labs USA Inc., Menlo Park, CA, 94025, USADepartment of Preventive Medicine, College of Medicine, Chungbuk National University, Cheongju, South KoreaDepartment of Preventive Medicine, College of Medicine, Chungbuk National University, Cheongju, South KoreaDepartment of Hygienic Chemistry, Graduate School of Biomedical Science, Nagasaki University, Nagasaki, 852-8521, JapanDepartment of Preventive Medicine, College of Medicine, Chungbuk National University, Cheongju, South Korea; Chungbuk Regional Cancer Center, Chungbuk National University Hospital, Cheongju, South KoreaDepartment of Preventive Medicine, College of Medicine, Chungbuk National University, Cheongju, South Korea; Chungbuk Regional Cancer Center, Chungbuk National University Hospital, Cheongju, South Korea; Corresponding author.Nitropyrene (1-NP) is a specific indicator of exposure to diesel exhaust and is partly metabolized to 1-aminopyrene (1-AP) and N-acetyl-1-aminopyrene (1-NAAP), which are excreted in urine. This study was conducted to evaluate the effects of gene polymorphisms of metabolic enzymes for 1-NP on the urinary concentrations of 1-AP and 1-NAAP. The study participants were 70 South Koreans who were occupationally or environmentally exposed to diesel exhaust. To evaluate 1-NP exposure levels, we sampled airborne particulate matters with a personal air sampler and measured urinary 1-AP and 1-NAAP concentrations. The genetic polymorphisms of the 1-NP metabolic enzymes (CYP1A1, CYP1B1, EPHX1, NQO1, and NAT2) were determined by direct sequencing. The mean 1-NP exposure level was 20.40 pg/m3, and the mean urinary concentrations of 1-AP and 1-NAAP were 0.074 nM and 0.213 nM, respectively. The correlation coefficient between the 1-NP exposure level and urinary 1-AP concentrations was 0.0138 and that between the 1-NP exposure level and urinary 1-NAAP concentrations was 0.1493, and neither correlation coefficient was statistically significant. The correlation coefficient between the 1-NP exposure level and urinary 1-AP concentrations showed statistically significant differences according to the CYP1A1 and CYP1B1 genotypes, and that between the 1-NP exposure level and urinary 1-NAAP concentrations was significantly different according to the CYP1A1, CYP1B1, and NAT2 genotypes. The urinary concentration of 1-NAAP is a better biomarker for exposure to 1-NP or DEPs because the former is higher, easier to measure, and more strongly correlated with 1-NP exposure levels than that of 1-AP. The relationship between 1-NP exposure and urinary 1-AP or 1-NAAP concentration depends on the single nucleotide polymorphism types of CYP1A1, CYP1B1, NQO1, and NAT2.http://www.sciencedirect.com/science/article/pii/S2405844022014086Diesel exhaust1-nitropyrene1-aminopyreneN-acetyl-1-aminopyreneMetabolic enzymesGenetic polymorphism |
| spellingShingle | Jung-Kuk Yun Bolormaa Ochirpurev Sang-Yong Eom Akira Toriba Yong-Dae Kim Heon Kim Effects of gene polymorphisms of CYP1A1, CYP1B1, EPHX1, NQO1, and NAT2 on urinary 1-nitropyrene metabolite concentrations Heliyon Diesel exhaust 1-nitropyrene 1-aminopyrene N-acetyl-1-aminopyrene Metabolic enzymes Genetic polymorphism |
| title | Effects of gene polymorphisms of CYP1A1, CYP1B1, EPHX1, NQO1, and NAT2 on urinary 1-nitropyrene metabolite concentrations |
| title_full | Effects of gene polymorphisms of CYP1A1, CYP1B1, EPHX1, NQO1, and NAT2 on urinary 1-nitropyrene metabolite concentrations |
| title_fullStr | Effects of gene polymorphisms of CYP1A1, CYP1B1, EPHX1, NQO1, and NAT2 on urinary 1-nitropyrene metabolite concentrations |
| title_full_unstemmed | Effects of gene polymorphisms of CYP1A1, CYP1B1, EPHX1, NQO1, and NAT2 on urinary 1-nitropyrene metabolite concentrations |
| title_short | Effects of gene polymorphisms of CYP1A1, CYP1B1, EPHX1, NQO1, and NAT2 on urinary 1-nitropyrene metabolite concentrations |
| title_sort | effects of gene polymorphisms of cyp1a1 cyp1b1 ephx1 nqo1 and nat2 on urinary 1 nitropyrene metabolite concentrations |
| topic | Diesel exhaust 1-nitropyrene 1-aminopyrene N-acetyl-1-aminopyrene Metabolic enzymes Genetic polymorphism |
| url | http://www.sciencedirect.com/science/article/pii/S2405844022014086 |
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