Low expression of c-Myc protein predicts poor outcomes in patients with hepatocellular carcinoma after resection
Abstract Background Embryonic Liver Fodrin (ELF) is an adaptor protein of transforming growth factor (TGF-β) signaling cascade. Disruption of ELF results in mislocalization of Smad3 and Smad4, leading to compromised TGF-β signaling. c-Myc is an important oncogenic transcription factor, and the disru...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-04-01
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| Series: | BMC Cancer |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12885-018-4379-5 |
| _version_ | 1819102015733628928 |
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| author | Fei Ji Zhi-heng Zhang Yi Zhang Shun-Li Shen Qing-Hua Cao Long-Juan Zhang Shao-Qiang Li Bao-Gang Peng Li-Jian Liang Yun-Peng Hua |
| author_facet | Fei Ji Zhi-heng Zhang Yi Zhang Shun-Li Shen Qing-Hua Cao Long-Juan Zhang Shao-Qiang Li Bao-Gang Peng Li-Jian Liang Yun-Peng Hua |
| author_sort | Fei Ji |
| collection | DOAJ |
| description | Abstract Background Embryonic Liver Fodrin (ELF) is an adaptor protein of transforming growth factor (TGF-β) signaling cascade. Disruption of ELF results in mislocalization of Smad3 and Smad4, leading to compromised TGF-β signaling. c-Myc is an important oncogenic transcription factor, and the disruption of TGF-β signaling promotes c-Myc-induced hepatocellular carcinoma (HCC) carcinogenesis. However, the prognostic significance of c-Myc in HCC is less understood Methods The expression of c-Myc protein and mRNA were measured by immunohistochemistry (IHC) and qRT- PCR, respectively. IHC was performed to detect TGF-β1 and ELF expression in HCC tissues. Their relationship with clinicopathological factors and overall survival (OS) and disease free survival (DFS) were examined. Results The expression of c-Myc protein and mRNA in HCC tissues were significantly higher in HCC area than those in normal liver tissues. However, the expression were low compared with those adjacent to HCC area. c-Myc protein was independently predictive of DFS and OS, and it was negatively correlated with tumor size (P = 0.031), tumor number (P = 0.038), and recurrence (P = 0.001). Low c-Myc expression was associated with short-term recurrence and poor prognosis. The predictive value of c-Myc combined with TGF-β1 or/and ELF was higher than that of any other single marker. Low c-Myc, high TGF-β1 or/and low ELF expression was associated with the worst DFS and OS. Conclusions Low expression of c-Myc protein predicts poor outcomes in patients with HCC with hepatectomy. The combination of the expression of c-Myc, TGF-β1, and ELF can be used to accurately predict outcomes of patients with HCC. |
| first_indexed | 2024-12-22T01:27:51Z |
| format | Article |
| id | doaj.art-6c068f77deee48f8a153876fce547c69 |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-12-22T01:27:51Z |
| publishDate | 2018-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-6c068f77deee48f8a153876fce547c692022-12-21T18:43:33ZengBMCBMC Cancer1471-24072018-04-0118111410.1186/s12885-018-4379-5Low expression of c-Myc protein predicts poor outcomes in patients with hepatocellular carcinoma after resectionFei Ji0Zhi-heng Zhang1Yi Zhang2Shun-Li Shen3Qing-Hua Cao4Long-Juan Zhang5Shao-Qiang Li6Bao-Gang Peng7Li-Jian Liang8Yun-Peng Hua9Pediatric Surgery, The First Affiliated Hospital, Sun Yat-sen UniversityOrgan Transplant Center, The First Affiliated Hospital, Sun Yat-sen UniversityDepartment of Hepatobiliary Surgery, The Third Affiliated Hospital, Sun Yat-sen UniversityDepartment of Liver Surgery, The First Affiliated Hospital, Sun Yat-sen UniversityDepartment of Pathology, The First Affiliated Hospital, Sun Yat-sen UniversityLaboratory of Surgery, The First Affiliated Hospital, Sun Yat-sen UniversityDepartment of Liver Surgery, The First Affiliated Hospital, Sun Yat-sen UniversityDepartment of Liver Surgery, The First Affiliated Hospital, Sun Yat-sen UniversityDepartment of Liver Surgery, The First Affiliated Hospital, Sun Yat-sen UniversityDepartment of Liver Surgery, The First Affiliated Hospital, Sun Yat-sen UniversityAbstract Background Embryonic Liver Fodrin (ELF) is an adaptor protein of transforming growth factor (TGF-β) signaling cascade. Disruption of ELF results in mislocalization of Smad3 and Smad4, leading to compromised TGF-β signaling. c-Myc is an important oncogenic transcription factor, and the disruption of TGF-β signaling promotes c-Myc-induced hepatocellular carcinoma (HCC) carcinogenesis. However, the prognostic significance of c-Myc in HCC is less understood Methods The expression of c-Myc protein and mRNA were measured by immunohistochemistry (IHC) and qRT- PCR, respectively. IHC was performed to detect TGF-β1 and ELF expression in HCC tissues. Their relationship with clinicopathological factors and overall survival (OS) and disease free survival (DFS) were examined. Results The expression of c-Myc protein and mRNA in HCC tissues were significantly higher in HCC area than those in normal liver tissues. However, the expression were low compared with those adjacent to HCC area. c-Myc protein was independently predictive of DFS and OS, and it was negatively correlated with tumor size (P = 0.031), tumor number (P = 0.038), and recurrence (P = 0.001). Low c-Myc expression was associated with short-term recurrence and poor prognosis. The predictive value of c-Myc combined with TGF-β1 or/and ELF was higher than that of any other single marker. Low c-Myc, high TGF-β1 or/and low ELF expression was associated with the worst DFS and OS. Conclusions Low expression of c-Myc protein predicts poor outcomes in patients with HCC with hepatectomy. The combination of the expression of c-Myc, TGF-β1, and ELF can be used to accurately predict outcomes of patients with HCC.http://link.springer.com/article/10.1186/s12885-018-4379-5C-MYCOncogeneHepatocellular carcinomaPrognosisBiomarkers |
| spellingShingle | Fei Ji Zhi-heng Zhang Yi Zhang Shun-Li Shen Qing-Hua Cao Long-Juan Zhang Shao-Qiang Li Bao-Gang Peng Li-Jian Liang Yun-Peng Hua Low expression of c-Myc protein predicts poor outcomes in patients with hepatocellular carcinoma after resection BMC Cancer C-MYC Oncogene Hepatocellular carcinoma Prognosis Biomarkers |
| title | Low expression of c-Myc protein predicts poor outcomes in patients with hepatocellular carcinoma after resection |
| title_full | Low expression of c-Myc protein predicts poor outcomes in patients with hepatocellular carcinoma after resection |
| title_fullStr | Low expression of c-Myc protein predicts poor outcomes in patients with hepatocellular carcinoma after resection |
| title_full_unstemmed | Low expression of c-Myc protein predicts poor outcomes in patients with hepatocellular carcinoma after resection |
| title_short | Low expression of c-Myc protein predicts poor outcomes in patients with hepatocellular carcinoma after resection |
| title_sort | low expression of c myc protein predicts poor outcomes in patients with hepatocellular carcinoma after resection |
| topic | C-MYC Oncogene Hepatocellular carcinoma Prognosis Biomarkers |
| url | http://link.springer.com/article/10.1186/s12885-018-4379-5 |
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