Miniature Inverted-Repeat Transposable Elements (MITEs) in the Two Lepidopteran Genomes of <i>Helicoverpa armigera</i> and <i>Helicoverpa zea</i>
Miniature inverted-repeat transposable elements MITEs are ubiquitous, non-autonomous class II transposable elements. The moths, <i>Helicoverpa armigera</i> and <i>Helicoverpa zea</i>, are recognized as the two most serious pest species within the genus. Moreover, these pests...
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MDPI AG
2022-03-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/13/4/313 |
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| author | Khouloud Klai Marwa Zidi Benoît Chénais Françoise Denis Aurore Caruso Nathalie Casse Maha Mezghani Khemakhem |
| author_facet | Khouloud Klai Marwa Zidi Benoît Chénais Françoise Denis Aurore Caruso Nathalie Casse Maha Mezghani Khemakhem |
| author_sort | Khouloud Klai |
| collection | DOAJ |
| description | Miniature inverted-repeat transposable elements MITEs are ubiquitous, non-autonomous class II transposable elements. The moths, <i>Helicoverpa armigera</i> and <i>Helicoverpa zea</i>, are recognized as the two most serious pest species within the genus. Moreover, these pests have the ability to develop insecticide resistance. In the present study, we conducted a genome-wide analysis of MITEs present in <i>H. armigera</i> and <i>H. zea</i> genomes using the bioinformatics tool, MITE tracker. Overall, 3570 and 7405 MITE sequences were identified in <i>H. armigera</i> and <i>H. zea</i> genomes, respectively. Comparative analysis of identified MITE sequences in the two genomes led to the identification of 18 families, comprising 140 MITE members in <i>H. armigera</i> and 161 MITE members in <i>H. zea.</i> Based on target site duplication (TSD) sequences, the identified families were classified into three superfamilies (PIF/harbinger, Tc1/mariner and CACTA). Copy numbers varied from 6 to 469 for each MITE family. Finally, the analysis of MITE insertion sites in defensome genes showed intronic insertions of 11 MITEs in the cytochrome P450, ATP-binding cassette transporter (ABC) and esterase genes in <i>H. armigera</i> whereas for <i>H. zea</i>, only one MITE was retrieved in the ABC-C2 gene. These insertions could thus be involved in the insecticide resistance observed in these pests. |
| first_indexed | 2024-03-09T13:31:11Z |
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| language | English |
| last_indexed | 2024-03-09T13:31:11Z |
| publishDate | 2022-03-01 |
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| series | Insects |
| spelling | doaj.art-6c06e00fc16140158b9630a4415bb5f22023-11-30T21:17:44ZengMDPI AGInsects2075-44502022-03-0113431310.3390/insects13040313Miniature Inverted-Repeat Transposable Elements (MITEs) in the Two Lepidopteran Genomes of <i>Helicoverpa armigera</i> and <i>Helicoverpa zea</i>Khouloud Klai0Marwa Zidi1Benoît Chénais2Françoise Denis3Aurore Caruso4Nathalie Casse5Maha Mezghani Khemakhem6Laboratory of Biochemistry and Biotechnology (LR01ES05), Faculty of Sciences of Tunis, University of Tunis El Manar, Tunis 1068, TunisiaLaboratory of Biochemistry and Biotechnology (LR01ES05), Faculty of Sciences of Tunis, University of Tunis El Manar, Tunis 1068, TunisiaLaboratory of Biology of Organisms, Stress, Health, Environment (BiOSSE), Le Mans University, 72085 Le Mans, FranceLaboratory of Biology of Organisms, Stress, Health, Environment (BiOSSE), Le Mans University, 72085 Le Mans, FranceLaboratory of Biology of Organisms, Stress, Health, Environment (BiOSSE), Le Mans University, 72085 Le Mans, FranceLaboratory of Biology of Organisms, Stress, Health, Environment (BiOSSE), Le Mans University, 72085 Le Mans, FranceLaboratory of Biochemistry and Biotechnology (LR01ES05), Faculty of Sciences of Tunis, University of Tunis El Manar, Tunis 1068, TunisiaMiniature inverted-repeat transposable elements MITEs are ubiquitous, non-autonomous class II transposable elements. The moths, <i>Helicoverpa armigera</i> and <i>Helicoverpa zea</i>, are recognized as the two most serious pest species within the genus. Moreover, these pests have the ability to develop insecticide resistance. In the present study, we conducted a genome-wide analysis of MITEs present in <i>H. armigera</i> and <i>H. zea</i> genomes using the bioinformatics tool, MITE tracker. Overall, 3570 and 7405 MITE sequences were identified in <i>H. armigera</i> and <i>H. zea</i> genomes, respectively. Comparative analysis of identified MITE sequences in the two genomes led to the identification of 18 families, comprising 140 MITE members in <i>H. armigera</i> and 161 MITE members in <i>H. zea.</i> Based on target site duplication (TSD) sequences, the identified families were classified into three superfamilies (PIF/harbinger, Tc1/mariner and CACTA). Copy numbers varied from 6 to 469 for each MITE family. Finally, the analysis of MITE insertion sites in defensome genes showed intronic insertions of 11 MITEs in the cytochrome P450, ATP-binding cassette transporter (ABC) and esterase genes in <i>H. armigera</i> whereas for <i>H. zea</i>, only one MITE was retrieved in the ABC-C2 gene. These insertions could thus be involved in the insecticide resistance observed in these pests.https://www.mdpi.com/2075-4450/13/4/313miniature inverted-repeats transposable elements<i>Helicoverpa armigera</i><i>Helicoverpa zea</i>insecticide resistance |
| spellingShingle | Khouloud Klai Marwa Zidi Benoît Chénais Françoise Denis Aurore Caruso Nathalie Casse Maha Mezghani Khemakhem Miniature Inverted-Repeat Transposable Elements (MITEs) in the Two Lepidopteran Genomes of <i>Helicoverpa armigera</i> and <i>Helicoverpa zea</i> Insects miniature inverted-repeats transposable elements <i>Helicoverpa armigera</i> <i>Helicoverpa zea</i> insecticide resistance |
| title | Miniature Inverted-Repeat Transposable Elements (MITEs) in the Two Lepidopteran Genomes of <i>Helicoverpa armigera</i> and <i>Helicoverpa zea</i> |
| title_full | Miniature Inverted-Repeat Transposable Elements (MITEs) in the Two Lepidopteran Genomes of <i>Helicoverpa armigera</i> and <i>Helicoverpa zea</i> |
| title_fullStr | Miniature Inverted-Repeat Transposable Elements (MITEs) in the Two Lepidopteran Genomes of <i>Helicoverpa armigera</i> and <i>Helicoverpa zea</i> |
| title_full_unstemmed | Miniature Inverted-Repeat Transposable Elements (MITEs) in the Two Lepidopteran Genomes of <i>Helicoverpa armigera</i> and <i>Helicoverpa zea</i> |
| title_short | Miniature Inverted-Repeat Transposable Elements (MITEs) in the Two Lepidopteran Genomes of <i>Helicoverpa armigera</i> and <i>Helicoverpa zea</i> |
| title_sort | miniature inverted repeat transposable elements mites in the two lepidopteran genomes of i helicoverpa armigera i and i helicoverpa zea i |
| topic | miniature inverted-repeats transposable elements <i>Helicoverpa armigera</i> <i>Helicoverpa zea</i> insecticide resistance |
| url | https://www.mdpi.com/2075-4450/13/4/313 |
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