A method for measuring meaningful physiological variables in fish blood without surgical cannulation
Abstract Gaining meaningful blood samples from water-breathing fish is a significant challenge. Two main methods typically used are grab ‘n’ stab and surgical cannulation. Both methods have benefits, but also significant limitations under various scenarios. Here we present a method of blood sampling...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-28061-w |
| _version_ | 1797945953538277376 |
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| author | William George. Davison Christopher A. Cooper Katherine A. Sloman Rod W. Wilson |
| author_facet | William George. Davison Christopher A. Cooper Katherine A. Sloman Rod W. Wilson |
| author_sort | William George. Davison |
| collection | DOAJ |
| description | Abstract Gaining meaningful blood samples from water-breathing fish is a significant challenge. Two main methods typically used are grab ‘n’ stab and surgical cannulation. Both methods have benefits, but also significant limitations under various scenarios. Here we present a method of blood sampling laboratory fish involving gradual induction of anaesthesia within their home tank, avoiding physical struggling associated with capture, followed by rapid transfer to a gill irrigation system to maintain artificial ventilation via adequate gill water flow and then followed by sampling the caudal vasculature. This method negates many blood chemistry disturbances associated with grab ‘n’ stab (i.e., low pH and oxygen, elevated lactate, CO2 and stress hormones) and generates results that are directly comparable to cannulated fish under a wide range of experimentally-induced acid–base scenarios (acidosis and alkalosis). Crucially this method was successful in achieving accurate acid–base blood measurements from fish ten times smaller than are typically suitable for cannulation. This opens opportunities not previously possible for studies that relate to basic physiology, sustainable aquaculture, ecotoxicology, conservation, and climate change. |
| first_indexed | 2024-04-10T21:03:12Z |
| format | Article |
| id | doaj.art-6c0775c0bf41440b92a4025479a4b183 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-10T21:03:12Z |
| publishDate | 2023-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-6c0775c0bf41440b92a4025479a4b1832023-01-22T12:13:02ZengNature PortfolioScientific Reports2045-23222023-01-0113111210.1038/s41598-023-28061-wA method for measuring meaningful physiological variables in fish blood without surgical cannulationWilliam George. Davison0Christopher A. Cooper1Katherine A. Sloman2Rod W. Wilson3Biosciences Department, College of Life and Environmental Sciences, University of ExeterInternational Zinc AssociationInstitute for Biomedical and Environmental Health Research, University of the West of ScotlandBiosciences Department, College of Life and Environmental Sciences, University of ExeterAbstract Gaining meaningful blood samples from water-breathing fish is a significant challenge. Two main methods typically used are grab ‘n’ stab and surgical cannulation. Both methods have benefits, but also significant limitations under various scenarios. Here we present a method of blood sampling laboratory fish involving gradual induction of anaesthesia within their home tank, avoiding physical struggling associated with capture, followed by rapid transfer to a gill irrigation system to maintain artificial ventilation via adequate gill water flow and then followed by sampling the caudal vasculature. This method negates many blood chemistry disturbances associated with grab ‘n’ stab (i.e., low pH and oxygen, elevated lactate, CO2 and stress hormones) and generates results that are directly comparable to cannulated fish under a wide range of experimentally-induced acid–base scenarios (acidosis and alkalosis). Crucially this method was successful in achieving accurate acid–base blood measurements from fish ten times smaller than are typically suitable for cannulation. This opens opportunities not previously possible for studies that relate to basic physiology, sustainable aquaculture, ecotoxicology, conservation, and climate change.https://doi.org/10.1038/s41598-023-28061-w |
| spellingShingle | William George. Davison Christopher A. Cooper Katherine A. Sloman Rod W. Wilson A method for measuring meaningful physiological variables in fish blood without surgical cannulation Scientific Reports |
| title | A method for measuring meaningful physiological variables in fish blood without surgical cannulation |
| title_full | A method for measuring meaningful physiological variables in fish blood without surgical cannulation |
| title_fullStr | A method for measuring meaningful physiological variables in fish blood without surgical cannulation |
| title_full_unstemmed | A method for measuring meaningful physiological variables in fish blood without surgical cannulation |
| title_short | A method for measuring meaningful physiological variables in fish blood without surgical cannulation |
| title_sort | method for measuring meaningful physiological variables in fish blood without surgical cannulation |
| url | https://doi.org/10.1038/s41598-023-28061-w |
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