Early Development of Definitive Erythroblasts from Human Pluripotent Stem Cells Defined by Expression of Glycophorin A/CD235a, CD34, and CD36
The development of human erythroid cells has been mostly examined in models of adult hematopoiesis, while their early derivation during embryonic and fetal stages is largely unknown. We observed the development and maturation of erythroblasts derived from human pluripotent stem cells (hPSCs) by an e...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2016-11-01
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| Series: | Stem Cell Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213671116301886 |
| _version_ | 1811337079276175360 |
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| author | Bin Mao Shu Huang Xulin Lu Wencui Sun Ya Zhou Xu Pan Jinfeng Yu Mowen Lai Bo Chen Qiongxiu Zhou Song Mao Guohui Bian Jiaxi Zhou Tatsutoshi Nakahata Feng Ma |
| author_facet | Bin Mao Shu Huang Xulin Lu Wencui Sun Ya Zhou Xu Pan Jinfeng Yu Mowen Lai Bo Chen Qiongxiu Zhou Song Mao Guohui Bian Jiaxi Zhou Tatsutoshi Nakahata Feng Ma |
| author_sort | Bin Mao |
| collection | DOAJ |
| description | The development of human erythroid cells has been mostly examined in models of adult hematopoiesis, while their early derivation during embryonic and fetal stages is largely unknown. We observed the development and maturation of erythroblasts derived from human pluripotent stem cells (hPSCs) by an efficient co-culture system. These hPSC-derived early erythroblasts initially showed definitive characteristics with a glycophorin A+ (GPA+) CD34lowCD36− phenotype and were distinct from adult CD34+ cell-derived ones. After losing CD34 expression, early GPA+CD36− erythroblasts matured into GPA+CD36low/+ stage as the latter expressed higher levels of β-globin along with a gradual loss of mesodermal and endothelial properties, and terminally suppressed CD36. We establish a unique in vitro model to trace the early development of hPSC-derived erythroblasts by serial expression of CD34, GPA, and CD36. Our findings may provide insight into the understanding of human early erythropoiesis and, ultimately, therapeutic potential. |
| first_indexed | 2024-04-13T17:49:44Z |
| format | Article |
| id | doaj.art-6c09ca74d24e4feaad6b2703be7b64f6 |
| institution | Directory Open Access Journal |
| issn | 2213-6711 |
| language | English |
| last_indexed | 2024-04-13T17:49:44Z |
| publishDate | 2016-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Reports |
| spelling | doaj.art-6c09ca74d24e4feaad6b2703be7b64f62022-12-22T02:36:46ZengElsevierStem Cell Reports2213-67112016-11-017586988310.1016/j.stemcr.2016.09.002Early Development of Definitive Erythroblasts from Human Pluripotent Stem Cells Defined by Expression of Glycophorin A/CD235a, CD34, and CD36Bin Mao0Shu Huang1Xulin Lu2Wencui Sun3Ya Zhou4Xu Pan5Jinfeng Yu6Mowen Lai7Bo Chen8Qiongxiu Zhou9Song Mao10Guohui Bian11Jiaxi Zhou12Tatsutoshi Nakahata13Feng Ma14Center for Stem Cell Research and Application, Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College (CAMS & PUMC), Chengdu 610052, ChinaCenter for Stem Cell Research and Application, Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College (CAMS & PUMC), Chengdu 610052, ChinaCenter for Stem Cell Research and Application, Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College (CAMS & PUMC), Chengdu 610052, ChinaCenter for Stem Cell Research and Application, Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College (CAMS & PUMC), Chengdu 610052, ChinaCenter for Stem Cell Research and Application, Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College (CAMS & PUMC), Chengdu 610052, ChinaCenter for Stem Cell Research and Application, Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College (CAMS & PUMC), Chengdu 610052, ChinaCenter for Stem Cell Research and Application, Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College (CAMS & PUMC), Chengdu 610052, ChinaCenter for Stem Cell Research and Application, Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College (CAMS & PUMC), Chengdu 610052, ChinaCenter for Stem Cell Research and Application, Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College (CAMS & PUMC), Chengdu 610052, ChinaCenter for Stem Cell Research and Application, Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College (CAMS & PUMC), Chengdu 610052, ChinaResearch Center for Stem Cell and Regenerative Medicine, Sichuan Neo-life Stem Cell Biotech Inc., Chengdu 610036, ChinaCenter for Stem Cell Research and Application, Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College (CAMS & PUMC), Chengdu 610052, ChinaState Key Lab of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, CAMS & PUMC, Tianjin 300020, ChinaDepartment of Clinical Application, Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto 606-8507, JapanCenter for Stem Cell Research and Application, Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College (CAMS & PUMC), Chengdu 610052, ChinaThe development of human erythroid cells has been mostly examined in models of adult hematopoiesis, while their early derivation during embryonic and fetal stages is largely unknown. We observed the development and maturation of erythroblasts derived from human pluripotent stem cells (hPSCs) by an efficient co-culture system. These hPSC-derived early erythroblasts initially showed definitive characteristics with a glycophorin A+ (GPA+) CD34lowCD36− phenotype and were distinct from adult CD34+ cell-derived ones. After losing CD34 expression, early GPA+CD36− erythroblasts matured into GPA+CD36low/+ stage as the latter expressed higher levels of β-globin along with a gradual loss of mesodermal and endothelial properties, and terminally suppressed CD36. We establish a unique in vitro model to trace the early development of hPSC-derived erythroblasts by serial expression of CD34, GPA, and CD36. Our findings may provide insight into the understanding of human early erythropoiesis and, ultimately, therapeutic potential.http://www.sciencedirect.com/science/article/pii/S2213671116301886hESChiPSChematopoiesisdevelopmenterythroblastserythropoiesisendothelial cellsGPACD36CD34 |
| spellingShingle | Bin Mao Shu Huang Xulin Lu Wencui Sun Ya Zhou Xu Pan Jinfeng Yu Mowen Lai Bo Chen Qiongxiu Zhou Song Mao Guohui Bian Jiaxi Zhou Tatsutoshi Nakahata Feng Ma Early Development of Definitive Erythroblasts from Human Pluripotent Stem Cells Defined by Expression of Glycophorin A/CD235a, CD34, and CD36 Stem Cell Reports hESC hiPSC hematopoiesis development erythroblasts erythropoiesis endothelial cells GPA CD36 CD34 |
| title | Early Development of Definitive Erythroblasts from Human Pluripotent Stem Cells Defined by Expression of Glycophorin A/CD235a, CD34, and CD36 |
| title_full | Early Development of Definitive Erythroblasts from Human Pluripotent Stem Cells Defined by Expression of Glycophorin A/CD235a, CD34, and CD36 |
| title_fullStr | Early Development of Definitive Erythroblasts from Human Pluripotent Stem Cells Defined by Expression of Glycophorin A/CD235a, CD34, and CD36 |
| title_full_unstemmed | Early Development of Definitive Erythroblasts from Human Pluripotent Stem Cells Defined by Expression of Glycophorin A/CD235a, CD34, and CD36 |
| title_short | Early Development of Definitive Erythroblasts from Human Pluripotent Stem Cells Defined by Expression of Glycophorin A/CD235a, CD34, and CD36 |
| title_sort | early development of definitive erythroblasts from human pluripotent stem cells defined by expression of glycophorin a cd235a cd34 and cd36 |
| topic | hESC hiPSC hematopoiesis development erythroblasts erythropoiesis endothelial cells GPA CD36 CD34 |
| url | http://www.sciencedirect.com/science/article/pii/S2213671116301886 |
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