MiR‐138‐5p suppresses the progression of lung cancer by targeting SNIP1
Abstract Background MicroRNAs (miRNAs) play crucial roles in the development of various cancers. Here, we aimed to evaluate the roles of miR‐138‐5p in lung cancer progression and the value of miR‐138‐5p in lung cancer diagnosis. Methods Quantitative real‐time PCR was performed to examine the express...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-02-01
|
| Series: | Thoracic Cancer |
| Subjects: | |
| Online Access: | https://doi.org/10.1111/1759-7714.14791 |
| _version_ | 1797894398434869248 |
|---|---|
| author | Jiaen Wu Xuejia Han Xiancong Yang Youjie Li Yan Liang Guangbin Sun Ranran Wang Pingyu Wang Shuyang Xie Jiankai Feng Hongfang Sun |
| author_facet | Jiaen Wu Xuejia Han Xiancong Yang Youjie Li Yan Liang Guangbin Sun Ranran Wang Pingyu Wang Shuyang Xie Jiankai Feng Hongfang Sun |
| author_sort | Jiaen Wu |
| collection | DOAJ |
| description | Abstract Background MicroRNAs (miRNAs) play crucial roles in the development of various cancers. Here, we aimed to evaluate the roles of miR‐138‐5p in lung cancer progression and the value of miR‐138‐5p in lung cancer diagnosis. Methods Quantitative real‐time PCR was performed to examine the expressions of miR‐138‐5p and smad nuclear interacting protein 1 (SNIP1) mRNA. The diagnostic value of miR‐138‐5p was analyzed using receiver operating characteristic (ROC) curve analysis, sensitivity, and specificity. We explored the effect of miR‐138‐5p on cell proliferation and metastasis by CCK‐8, colony formation, wound healing and transwell assays. Western blot was employed to detect the protein expression of SNIP1 and related genes. Lung cancer cell growth was evaluated in vivo using xenograft tumor assay. Results MiR‐138‐5p was decreased in the serum of patients with non‐small cell lung cancer (NSCLC) and in NSCLC cells and tissues. The area under the ROC curve of serum miR‐138‐5p in the diagnosis of NSCLC was 0.922. This finding indicates the high diagnostic efficiency for lung cancer. MiR‐138‐5p suppressed but its inhibitor promoted cell proliferation and migration compared with control treatment in vitro and in vivo. MiR‐138‐5p directly binds to the 3′‐untranslated region of SNIP1 and negatively regulated the expression of SNIP1, thereby inhibiting the expression of cyclin D1 and c‐Myc. Moreover, overexpression of SNIP1 rescues the miR‐138‐5p‐mediated inhibition in NSCLC cells. Conclusions The results suggested that miR‐138‐5p suppressed lung cancer cell proliferation and migration by targeting SNIP1. Serum miR‐138‐5p is a novel and valuable biomarker for NSCLC diagnosis. |
| first_indexed | 2024-04-10T07:08:25Z |
| format | Article |
| id | doaj.art-6c0f0b55b02d4cdbb0693cf5ff4f099a |
| institution | Directory Open Access Journal |
| issn | 1759-7706 1759-7714 |
| language | English |
| last_indexed | 2024-04-10T07:08:25Z |
| publishDate | 2023-02-01 |
| publisher | Wiley |
| record_format | Article |
| series | Thoracic Cancer |
| spelling | doaj.art-6c0f0b55b02d4cdbb0693cf5ff4f099a2023-02-27T01:36:59ZengWileyThoracic Cancer1759-77061759-77142023-02-0114661262310.1111/1759-7714.14791MiR‐138‐5p suppresses the progression of lung cancer by targeting SNIP1Jiaen Wu0Xuejia Han1Xiancong Yang2Youjie Li3Yan Liang4Guangbin Sun5Ranran Wang6Pingyu Wang7Shuyang Xie8Jiankai Feng9Hongfang Sun10Department of Biochemistry and Molecular Biology Binzhou Medical University Yantai ChinaDepartment of Biochemistry and Molecular Biology Binzhou Medical University Yantai ChinaDepartment of Biochemistry and Molecular Biology Binzhou Medical University Yantai ChinaDepartment of Biochemistry and Molecular Biology Binzhou Medical University Yantai ChinaDepartment of Biochemistry and Molecular Biology Binzhou Medical University Yantai ChinaDepartment of Biochemistry and Molecular Biology Binzhou Medical University Yantai ChinaDepartment of Biochemistry and Molecular Biology Binzhou Medical University Yantai ChinaDepartment of Biochemistry and Molecular Biology Binzhou Medical University Yantai ChinaDepartment of Biochemistry and Molecular Biology Binzhou Medical University Yantai ChinaDepartment of Laboratory Medicine Yantai Affiliated Hospital of Binzhou Medical University Yantai ChinaDepartment of Biochemistry and Molecular Biology Binzhou Medical University Yantai ChinaAbstract Background MicroRNAs (miRNAs) play crucial roles in the development of various cancers. Here, we aimed to evaluate the roles of miR‐138‐5p in lung cancer progression and the value of miR‐138‐5p in lung cancer diagnosis. Methods Quantitative real‐time PCR was performed to examine the expressions of miR‐138‐5p and smad nuclear interacting protein 1 (SNIP1) mRNA. The diagnostic value of miR‐138‐5p was analyzed using receiver operating characteristic (ROC) curve analysis, sensitivity, and specificity. We explored the effect of miR‐138‐5p on cell proliferation and metastasis by CCK‐8, colony formation, wound healing and transwell assays. Western blot was employed to detect the protein expression of SNIP1 and related genes. Lung cancer cell growth was evaluated in vivo using xenograft tumor assay. Results MiR‐138‐5p was decreased in the serum of patients with non‐small cell lung cancer (NSCLC) and in NSCLC cells and tissues. The area under the ROC curve of serum miR‐138‐5p in the diagnosis of NSCLC was 0.922. This finding indicates the high diagnostic efficiency for lung cancer. MiR‐138‐5p suppressed but its inhibitor promoted cell proliferation and migration compared with control treatment in vitro and in vivo. MiR‐138‐5p directly binds to the 3′‐untranslated region of SNIP1 and negatively regulated the expression of SNIP1, thereby inhibiting the expression of cyclin D1 and c‐Myc. Moreover, overexpression of SNIP1 rescues the miR‐138‐5p‐mediated inhibition in NSCLC cells. Conclusions The results suggested that miR‐138‐5p suppressed lung cancer cell proliferation and migration by targeting SNIP1. Serum miR‐138‐5p is a novel and valuable biomarker for NSCLC diagnosis.https://doi.org/10.1111/1759-7714.14791biomarkerlung cancermiR‐138‐5pSNIP1tumor suppressor |
| spellingShingle | Jiaen Wu Xuejia Han Xiancong Yang Youjie Li Yan Liang Guangbin Sun Ranran Wang Pingyu Wang Shuyang Xie Jiankai Feng Hongfang Sun MiR‐138‐5p suppresses the progression of lung cancer by targeting SNIP1 Thoracic Cancer biomarker lung cancer miR‐138‐5p SNIP1 tumor suppressor |
| title | MiR‐138‐5p suppresses the progression of lung cancer by targeting SNIP1 |
| title_full | MiR‐138‐5p suppresses the progression of lung cancer by targeting SNIP1 |
| title_fullStr | MiR‐138‐5p suppresses the progression of lung cancer by targeting SNIP1 |
| title_full_unstemmed | MiR‐138‐5p suppresses the progression of lung cancer by targeting SNIP1 |
| title_short | MiR‐138‐5p suppresses the progression of lung cancer by targeting SNIP1 |
| title_sort | mir 138 5p suppresses the progression of lung cancer by targeting snip1 |
| topic | biomarker lung cancer miR‐138‐5p SNIP1 tumor suppressor |
| url | https://doi.org/10.1111/1759-7714.14791 |
| work_keys_str_mv | AT jiaenwu mir1385psuppressestheprogressionoflungcancerbytargetingsnip1 AT xuejiahan mir1385psuppressestheprogressionoflungcancerbytargetingsnip1 AT xiancongyang mir1385psuppressestheprogressionoflungcancerbytargetingsnip1 AT youjieli mir1385psuppressestheprogressionoflungcancerbytargetingsnip1 AT yanliang mir1385psuppressestheprogressionoflungcancerbytargetingsnip1 AT guangbinsun mir1385psuppressestheprogressionoflungcancerbytargetingsnip1 AT ranranwang mir1385psuppressestheprogressionoflungcancerbytargetingsnip1 AT pingyuwang mir1385psuppressestheprogressionoflungcancerbytargetingsnip1 AT shuyangxie mir1385psuppressestheprogressionoflungcancerbytargetingsnip1 AT jiankaifeng mir1385psuppressestheprogressionoflungcancerbytargetingsnip1 AT hongfangsun mir1385psuppressestheprogressionoflungcancerbytargetingsnip1 |