Isolation of alkaline phosphatase enzyme from cactus plant Aloe Vera and study its properties
ABSTRACT<br /> The research was concerned with isolation of alkaline phosphatase (ALP) from the aqueous extract of Aloe Vera using gel filtration technique.<br /> It was shown that, using gel filtration chromatography on Sephadex G-100, the solution of the proteinous precipitate produced...
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| Format: | Article |
| Language: | Arabic |
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College of Education for Pure Sciences
2007-04-01
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| Series: | مجلة التربية والعلم |
| Subjects: | |
| Online Access: | https://edusj.mosuljournals.com/article_51310_9b562d8cb32240a7bd9a143e852235e3.pdf |
| _version_ | 1818120198693060608 |
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| author | Sakina Rashid |
| author_facet | Sakina Rashid |
| author_sort | Sakina Rashid |
| collection | DOAJ |
| description | ABSTRACT<br /> The research was concerned with isolation of alkaline phosphatase (ALP) from the aqueous extract of Aloe Vera using gel filtration technique.<br /> It was shown that, using gel filtration chromatography on Sephadex G-100, the solution of the proteinous precipitate produced by ammonium sulphate saturation, contained two proteinous peaks. The two peaks possessed a variable activity of alkaline phosphatase, where maximum specific activity was obtained in the first peak with higher molecular weight which showed (28) folds of purification.<br /> Furthermore, the comparative molecular weight of the partially purified alkaline phosphatase (first proteinous peak) using gel filtration was found to be (147353) Dalton. <br /> The research was also concerned with finding the optimum conditions of alkaline phosphatase. Maximum activity was obtained using sodium carbonate- bicarbonate buffer (100 mM) at pH ( 9.7 ), (50ºC) and (30 mM) of di-sodium phenyl phosphate as a substrate.<br /> Using linweaver-Burk plot, it was found that the Vmax and Km have the values of (185.18 enzyme unit/dl of proteinous solution) and (3.9mM) respectively.<br /> The results also predicted that the activity of alkaline phosphatase decreased gradually and reached 76% and 50% from the original value when stored at 4ºC and at room temperature (33-40ºC) respectively for 30 days. |
| first_indexed | 2024-12-11T05:22:17Z |
| format | Article |
| id | doaj.art-6c105873de5f4f1fae9680ea0f0272c2 |
| institution | Directory Open Access Journal |
| issn | 1812-125X 2664-2530 |
| language | Arabic |
| last_indexed | 2024-12-11T05:22:17Z |
| publishDate | 2007-04-01 |
| publisher | College of Education for Pure Sciences |
| record_format | Article |
| series | مجلة التربية والعلم |
| spelling | doaj.art-6c105873de5f4f1fae9680ea0f0272c22022-12-22T01:19:40ZaraCollege of Education for Pure Sciencesمجلة التربية والعلم1812-125X2664-25302007-04-0119311510.33899/edusj.2007.5131051310Isolation of alkaline phosphatase enzyme from cactus plant Aloe Vera and study its propertiesSakina RashidABSTRACT<br /> The research was concerned with isolation of alkaline phosphatase (ALP) from the aqueous extract of Aloe Vera using gel filtration technique.<br /> It was shown that, using gel filtration chromatography on Sephadex G-100, the solution of the proteinous precipitate produced by ammonium sulphate saturation, contained two proteinous peaks. The two peaks possessed a variable activity of alkaline phosphatase, where maximum specific activity was obtained in the first peak with higher molecular weight which showed (28) folds of purification.<br /> Furthermore, the comparative molecular weight of the partially purified alkaline phosphatase (first proteinous peak) using gel filtration was found to be (147353) Dalton. <br /> The research was also concerned with finding the optimum conditions of alkaline phosphatase. Maximum activity was obtained using sodium carbonate- bicarbonate buffer (100 mM) at pH ( 9.7 ), (50ºC) and (30 mM) of di-sodium phenyl phosphate as a substrate.<br /> Using linweaver-Burk plot, it was found that the Vmax and Km have the values of (185.18 enzyme unit/dl of proteinous solution) and (3.9mM) respectively.<br /> The results also predicted that the activity of alkaline phosphatase decreased gradually and reached 76% and 50% from the original value when stored at 4ºC and at room temperature (33-40ºC) respectively for 30 days.https://edusj.mosuljournals.com/article_51310_9b562d8cb32240a7bd9a143e852235e3.pdfaloe veraalkaline phosphatase |
| spellingShingle | Sakina Rashid Isolation of alkaline phosphatase enzyme from cactus plant Aloe Vera and study its properties مجلة التربية والعلم aloe vera alkaline phosphatase |
| title | Isolation of alkaline phosphatase enzyme from cactus plant Aloe Vera and study its properties |
| title_full | Isolation of alkaline phosphatase enzyme from cactus plant Aloe Vera and study its properties |
| title_fullStr | Isolation of alkaline phosphatase enzyme from cactus plant Aloe Vera and study its properties |
| title_full_unstemmed | Isolation of alkaline phosphatase enzyme from cactus plant Aloe Vera and study its properties |
| title_short | Isolation of alkaline phosphatase enzyme from cactus plant Aloe Vera and study its properties |
| title_sort | isolation of alkaline phosphatase enzyme from cactus plant aloe vera and study its properties |
| topic | aloe vera alkaline phosphatase |
| url | https://edusj.mosuljournals.com/article_51310_9b562d8cb32240a7bd9a143e852235e3.pdf |
| work_keys_str_mv | AT sakinarashid isolationofalkalinephosphataseenzymefromcactusplantaloeveraandstudyitsproperties |