Exploration of the Esophageal Mucosal Barrier in Non-Erosive Reflux Disease
In the absence of visible mucosal damage, it is hypothesized that the esophageal mucosal barrier is functionally impaired in patients with non-erosive reflux disease (NERD). The aim of the present study was to perform an exploratory analysis of the mucosal barrier in NERD compared to erosive esophag...
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| Format: | Article |
| Language: | English |
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MDPI AG
2017-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | http://www.mdpi.com/1422-0067/18/5/1091 |
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| author | Nicolaas F. Rinsma Ricard Farré Fred J. Troost Montserrat Elizalde Daniel Keszthelyi Zsuzsanna Helyes Ad A. Masclee José M. Conchillo |
| author_facet | Nicolaas F. Rinsma Ricard Farré Fred J. Troost Montserrat Elizalde Daniel Keszthelyi Zsuzsanna Helyes Ad A. Masclee José M. Conchillo |
| author_sort | Nicolaas F. Rinsma |
| collection | DOAJ |
| description | In the absence of visible mucosal damage, it is hypothesized that the esophageal mucosal barrier is functionally impaired in patients with non-erosive reflux disease (NERD). The aim of the present study was to perform an exploratory analysis of the mucosal barrier in NERD compared to erosive esophagitis (EE) and controls. A second aim was to explore TRPV1 gene transcription in relation to the mucosal barrier function and heartburn symptoms. In this prospective study, 10 NERD patients, 11 patients with active erosive esophagitis and 10 healthy volunteers were included. Biopsies from non-eroded mucosa were obtained for (1) ex vivo analyses (Ussing chamber) of transepithelial electrical resistance (TEER) and permeability (2) gene transcription of tight-junction proteins and transient receptor potential vanilloid subfamily member 1 (TRPV1). No differences in TEER or permeability were found between NERD and healthy volunteers, whereas TEER was lower in patients with erosive esophagitis. TRPV1 gene transcription was not significantly different between EE, NERD and controls. Conclusions: esophageal mucosal barrier function and TRPV1 transcription is not significantly altered in NERD patients. Future research is needed to explore other potential mechanisms that may account for the high symptom burden in these patients. |
| first_indexed | 2024-04-12T06:43:42Z |
| format | Article |
| id | doaj.art-6c1912efd8474712b3560d9d786bd2d2 |
| institution | Directory Open Access Journal |
| issn | 1422-0067 |
| language | English |
| last_indexed | 2024-04-12T06:43:42Z |
| publishDate | 2017-05-01 |
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| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-6c1912efd8474712b3560d9d786bd2d22022-12-22T03:43:39ZengMDPI AGInternational Journal of Molecular Sciences1422-00672017-05-01185109110.3390/ijms18051091ijms18051091Exploration of the Esophageal Mucosal Barrier in Non-Erosive Reflux DiseaseNicolaas F. Rinsma0Ricard Farré1Fred J. Troost2Montserrat Elizalde3Daniel Keszthelyi4Zsuzsanna Helyes5Ad A. Masclee6José M. Conchillo7Division of Gastroenterology-Hepatology, Department of Internal Medicine, School of Nutrition and Translational Research in Metabolism (NUTRIM), Maastricht University Medical Center, 6229 HX Maastricht, The NetherlandsTranslational Research Center for Gastrointestinal Disorders, Catholic University Leuven, 3000 Leuven, BelgiumDivision of Gastroenterology-Hepatology, Department of Internal Medicine, School of Nutrition and Translational Research in Metabolism (NUTRIM), Maastricht University Medical Center, 6229 HX Maastricht, The NetherlandsDivision of Gastroenterology-Hepatology, Department of Internal Medicine, School of Nutrition and Translational Research in Metabolism (NUTRIM), Maastricht University Medical Center, 6229 HX Maastricht, The NetherlandsDivision of Gastroenterology-Hepatology, Department of Internal Medicine, School of Nutrition and Translational Research in Metabolism (NUTRIM), Maastricht University Medical Center, 6229 HX Maastricht, The NetherlandsDepartment of Pharmacology and Pharmacotherapy, University of Pécs, 7623 Pécs, HungaryDivision of Gastroenterology-Hepatology, Department of Internal Medicine, School of Nutrition and Translational Research in Metabolism (NUTRIM), Maastricht University Medical Center, 6229 HX Maastricht, The NetherlandsDivision of Gastroenterology-Hepatology, Department of Internal Medicine, School of Nutrition and Translational Research in Metabolism (NUTRIM), Maastricht University Medical Center, 6229 HX Maastricht, The NetherlandsIn the absence of visible mucosal damage, it is hypothesized that the esophageal mucosal barrier is functionally impaired in patients with non-erosive reflux disease (NERD). The aim of the present study was to perform an exploratory analysis of the mucosal barrier in NERD compared to erosive esophagitis (EE) and controls. A second aim was to explore TRPV1 gene transcription in relation to the mucosal barrier function and heartburn symptoms. In this prospective study, 10 NERD patients, 11 patients with active erosive esophagitis and 10 healthy volunteers were included. Biopsies from non-eroded mucosa were obtained for (1) ex vivo analyses (Ussing chamber) of transepithelial electrical resistance (TEER) and permeability (2) gene transcription of tight-junction proteins and transient receptor potential vanilloid subfamily member 1 (TRPV1). No differences in TEER or permeability were found between NERD and healthy volunteers, whereas TEER was lower in patients with erosive esophagitis. TRPV1 gene transcription was not significantly different between EE, NERD and controls. Conclusions: esophageal mucosal barrier function and TRPV1 transcription is not significantly altered in NERD patients. Future research is needed to explore other potential mechanisms that may account for the high symptom burden in these patients.http://www.mdpi.com/1422-0067/18/5/1091mucosal integrityUssing chambererosive esophagitisnon-erosive reflux diseasetransepithelial electrical resistanceTRPV1heartburn |
| spellingShingle | Nicolaas F. Rinsma Ricard Farré Fred J. Troost Montserrat Elizalde Daniel Keszthelyi Zsuzsanna Helyes Ad A. Masclee José M. Conchillo Exploration of the Esophageal Mucosal Barrier in Non-Erosive Reflux Disease International Journal of Molecular Sciences mucosal integrity Ussing chamber erosive esophagitis non-erosive reflux disease transepithelial electrical resistance TRPV1 heartburn |
| title | Exploration of the Esophageal Mucosal Barrier in Non-Erosive Reflux Disease |
| title_full | Exploration of the Esophageal Mucosal Barrier in Non-Erosive Reflux Disease |
| title_fullStr | Exploration of the Esophageal Mucosal Barrier in Non-Erosive Reflux Disease |
| title_full_unstemmed | Exploration of the Esophageal Mucosal Barrier in Non-Erosive Reflux Disease |
| title_short | Exploration of the Esophageal Mucosal Barrier in Non-Erosive Reflux Disease |
| title_sort | exploration of the esophageal mucosal barrier in non erosive reflux disease |
| topic | mucosal integrity Ussing chamber erosive esophagitis non-erosive reflux disease transepithelial electrical resistance TRPV1 heartburn |
| url | http://www.mdpi.com/1422-0067/18/5/1091 |
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