Silica Nanoparticles Disclose a Detailed Neurodegeneration Profile throughout the Life Span of a Model Organism
The incidence of age-related neurodegenerative diseases is rising globally. However, the temporal sequence of neurodegeneration throughout adult life is poorly understood. To identify the starting points and schedule of neurodegenerative events, serotonergic and dopaminergic neurons were monitored i...
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| Format: | Article |
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MDPI AG
2024-01-01
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| Series: | Journal of Xenobiotics |
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| Online Access: | https://www.mdpi.com/2039-4713/14/1/8 |
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| author | Annette Limke Gereon Poschmann Kai Stühler Patrick Petzsch Thorsten Wachtmeister Anna von Mikecz |
| author_facet | Annette Limke Gereon Poschmann Kai Stühler Patrick Petzsch Thorsten Wachtmeister Anna von Mikecz |
| author_sort | Annette Limke |
| collection | DOAJ |
| description | The incidence of age-related neurodegenerative diseases is rising globally. However, the temporal sequence of neurodegeneration throughout adult life is poorly understood. To identify the starting points and schedule of neurodegenerative events, serotonergic and dopaminergic neurons were monitored in the model organism <i>C. elegans</i>, which has a life span of 2–3 weeks. Neural morphology was examined from young to old nematodes that were exposed to silica nanoparticles. Young nematodes showed phenotypes such as dendritic beading of serotonergic and dopaminergic neurons that are normally not seen until late life. During aging, neurodegeneration spreads from specifically susceptible ADF and PDE neurons in young <i>C. elegans</i> to other more resilient neurons, such as dopaminergic CEP in middle-aged worms. Investigation of neurodegenerative hallmarks and animal behavior revealed a temporal correlation with the acceleration of neuromuscular defects, such as internal hatch in 2-day-old <i>C. elegans</i>. Transcriptomics and proteomics of young worms exposed to nano silica showed a change in gene expression concerning the gene ontology groups serotonergic and dopaminergic signaling as well as neuropeptide signaling. Consistent with this, reporter strains for nlp-3, nlp-14 and nlp-21 confirmed premature degeneration of the serotonergic neuron HSN and other neurons in young <i>C. elegans</i>. The results identify young nematodes as a vulnerable age group for nano silica-induced neural defects with a significantly reduced health span. Neurodegeneration of specific neurons impairs signaling by classical neurotransmitters as well as neuropeptides and compromises related neuromuscular behaviors in critical phases of life, such as the reproductive phase. |
| first_indexed | 2024-04-24T18:07:02Z |
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| issn | 2039-4705 2039-4713 |
| language | English |
| last_indexed | 2024-04-24T18:07:02Z |
| publishDate | 2024-01-01 |
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| record_format | Article |
| series | Journal of Xenobiotics |
| spelling | doaj.art-6c1b0ecc443e4ea9bf2ffb2b0428dc022024-03-27T13:49:55ZengMDPI AGJournal of Xenobiotics2039-47052039-47132024-01-0114113515310.3390/jox14010008Silica Nanoparticles Disclose a Detailed Neurodegeneration Profile throughout the Life Span of a Model OrganismAnnette Limke0Gereon Poschmann1Kai Stühler2Patrick Petzsch3Thorsten Wachtmeister4Anna von Mikecz5IUF–Leibniz Research Institute of Environmental Medicine GmbH, Auf’m Hennekamp 50, 40225 Düsseldorf, GermanyInstitute of Molecular Medicine, Proteome Research, Medical Faculty and University Hospital, Heinrich Heine University Düsseldorf, 40225 Düsseldorf, GermanyMolecular Proteomics Laboratory, BMFZ, Heinrich Heine University Düsseldorf, 40225 Düsseldorf, GermanyBiological and Medical Research Center (BMFZ), Medical Faculty, Heinrich-Heine-University, Universitätsstraße 1, 40225 Düsseldorf, GermanyBiological and Medical Research Center (BMFZ), Medical Faculty, Heinrich-Heine-University, Universitätsstraße 1, 40225 Düsseldorf, GermanyIUF–Leibniz Research Institute of Environmental Medicine GmbH, Auf’m Hennekamp 50, 40225 Düsseldorf, GermanyThe incidence of age-related neurodegenerative diseases is rising globally. However, the temporal sequence of neurodegeneration throughout adult life is poorly understood. To identify the starting points and schedule of neurodegenerative events, serotonergic and dopaminergic neurons were monitored in the model organism <i>C. elegans</i>, which has a life span of 2–3 weeks. Neural morphology was examined from young to old nematodes that were exposed to silica nanoparticles. Young nematodes showed phenotypes such as dendritic beading of serotonergic and dopaminergic neurons that are normally not seen until late life. During aging, neurodegeneration spreads from specifically susceptible ADF and PDE neurons in young <i>C. elegans</i> to other more resilient neurons, such as dopaminergic CEP in middle-aged worms. Investigation of neurodegenerative hallmarks and animal behavior revealed a temporal correlation with the acceleration of neuromuscular defects, such as internal hatch in 2-day-old <i>C. elegans</i>. Transcriptomics and proteomics of young worms exposed to nano silica showed a change in gene expression concerning the gene ontology groups serotonergic and dopaminergic signaling as well as neuropeptide signaling. Consistent with this, reporter strains for nlp-3, nlp-14 and nlp-21 confirmed premature degeneration of the serotonergic neuron HSN and other neurons in young <i>C. elegans</i>. The results identify young nematodes as a vulnerable age group for nano silica-induced neural defects with a significantly reduced health span. Neurodegeneration of specific neurons impairs signaling by classical neurotransmitters as well as neuropeptides and compromises related neuromuscular behaviors in critical phases of life, such as the reproductive phase.https://www.mdpi.com/2039-4713/14/1/8amyloid disease<i>C. elegans</i>dopaminenanomaterialnervous systemneuropeptides |
| spellingShingle | Annette Limke Gereon Poschmann Kai Stühler Patrick Petzsch Thorsten Wachtmeister Anna von Mikecz Silica Nanoparticles Disclose a Detailed Neurodegeneration Profile throughout the Life Span of a Model Organism Journal of Xenobiotics amyloid disease <i>C. elegans</i> dopamine nanomaterial nervous system neuropeptides |
| title | Silica Nanoparticles Disclose a Detailed Neurodegeneration Profile throughout the Life Span of a Model Organism |
| title_full | Silica Nanoparticles Disclose a Detailed Neurodegeneration Profile throughout the Life Span of a Model Organism |
| title_fullStr | Silica Nanoparticles Disclose a Detailed Neurodegeneration Profile throughout the Life Span of a Model Organism |
| title_full_unstemmed | Silica Nanoparticles Disclose a Detailed Neurodegeneration Profile throughout the Life Span of a Model Organism |
| title_short | Silica Nanoparticles Disclose a Detailed Neurodegeneration Profile throughout the Life Span of a Model Organism |
| title_sort | silica nanoparticles disclose a detailed neurodegeneration profile throughout the life span of a model organism |
| topic | amyloid disease <i>C. elegans</i> dopamine nanomaterial nervous system neuropeptides |
| url | https://www.mdpi.com/2039-4713/14/1/8 |
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