Differential gene expression in decidualized human endometrial stromal cells induced by different stimuli
Abstract Decidualization can be induced by culturing human endometrial stromal cells (ESCs) with several decidualization stimuli, such as cAMP, medroxyprogesterone acetate (MPA) or Estradiol (E2). However, it has been unclear how decidualized cells induced by different stimuli are different. We comp...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-04-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-58065-z |
| _version_ | 1797219869727391744 |
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| author | Yumiko Doi-Tanaka Isao Tamura Amon Shiroshita Taishi Fujimura Yuichiro Shirafuta Ryo Maekawa Toshiaki Taketani Shun Sato Norihiro Sugino |
| author_facet | Yumiko Doi-Tanaka Isao Tamura Amon Shiroshita Taishi Fujimura Yuichiro Shirafuta Ryo Maekawa Toshiaki Taketani Shun Sato Norihiro Sugino |
| author_sort | Yumiko Doi-Tanaka |
| collection | DOAJ |
| description | Abstract Decidualization can be induced by culturing human endometrial stromal cells (ESCs) with several decidualization stimuli, such as cAMP, medroxyprogesterone acetate (MPA) or Estradiol (E2). However, it has been unclear how decidualized cells induced by different stimuli are different. We compared transcriptomes and cellular functions of decidualized ESCs induced by different stimuli (MPA, E2 + MPA, cAMP, and cAMP + MPA). We also investigated which decidualization stimulus induces a closer in vivo decidualization. Differentially expressed genes (DEGs) and altered cellular functions by each decidualization stimuli were identified by RNA-sequence and gene-ontology analysis. DEGs was about two times higher for stimuli that use cAMP (cAMP and cAMP + MPA) than for stimuli that did not use cAMP (MPA and E2 + MPA). cAMP-using stimuli altered the cellular functions including angiogenesis, inflammation, immune system, and embryo implantation whereas MPA-using stimuli (MPA, E2 + MPA, and cAMP + MPA) altered the cellular functions associated with insulin signaling. A public single-cell RNA-sequence data of the human endometrium was utilized to analyze in vivo decidualization. The altered cellular functions by in vivo decidualization were close to those observed by cAMP + MPA-induced decidualization. In conclusion, decidualized cells induced by different stimuli have different transcriptome and cellular functions. cAMP + MPA may induce a decidualization most closely to in vivo decidualization. |
| first_indexed | 2024-04-24T12:40:30Z |
| format | Article |
| id | doaj.art-6c205fab67724478b54f09133dd49a1b |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-24T12:40:30Z |
| publishDate | 2024-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-6c205fab67724478b54f09133dd49a1b2024-04-07T11:15:19ZengNature PortfolioScientific Reports2045-23222024-04-0114111210.1038/s41598-024-58065-zDifferential gene expression in decidualized human endometrial stromal cells induced by different stimuliYumiko Doi-Tanaka0Isao Tamura1Amon Shiroshita2Taishi Fujimura3Yuichiro Shirafuta4Ryo Maekawa5Toshiaki Taketani6Shun Sato7Norihiro Sugino8Department of Obstetrics and Gynecology, Yamaguchi University Graduate School of MedicineDepartment of Obstetrics and Gynecology, Yamaguchi University Graduate School of MedicineDepartment of Obstetrics and Gynecology, Yamaguchi University Graduate School of MedicineDepartment of Obstetrics and Gynecology, Yamaguchi University Graduate School of MedicineDepartment of Obstetrics and Gynecology, Yamaguchi University Graduate School of MedicineDepartment of Obstetrics and Gynecology, Yamaguchi University Graduate School of MedicineDepartment of Obstetrics and Gynecology, Yamaguchi University Graduate School of MedicineDepartment of Obstetrics and Gynecology, Yamaguchi University Graduate School of MedicineDepartment of Obstetrics and Gynecology, Yamaguchi University Graduate School of MedicineAbstract Decidualization can be induced by culturing human endometrial stromal cells (ESCs) with several decidualization stimuli, such as cAMP, medroxyprogesterone acetate (MPA) or Estradiol (E2). However, it has been unclear how decidualized cells induced by different stimuli are different. We compared transcriptomes and cellular functions of decidualized ESCs induced by different stimuli (MPA, E2 + MPA, cAMP, and cAMP + MPA). We also investigated which decidualization stimulus induces a closer in vivo decidualization. Differentially expressed genes (DEGs) and altered cellular functions by each decidualization stimuli were identified by RNA-sequence and gene-ontology analysis. DEGs was about two times higher for stimuli that use cAMP (cAMP and cAMP + MPA) than for stimuli that did not use cAMP (MPA and E2 + MPA). cAMP-using stimuli altered the cellular functions including angiogenesis, inflammation, immune system, and embryo implantation whereas MPA-using stimuli (MPA, E2 + MPA, and cAMP + MPA) altered the cellular functions associated with insulin signaling. A public single-cell RNA-sequence data of the human endometrium was utilized to analyze in vivo decidualization. The altered cellular functions by in vivo decidualization were close to those observed by cAMP + MPA-induced decidualization. In conclusion, decidualized cells induced by different stimuli have different transcriptome and cellular functions. cAMP + MPA may induce a decidualization most closely to in vivo decidualization.https://doi.org/10.1038/s41598-024-58065-z |
| spellingShingle | Yumiko Doi-Tanaka Isao Tamura Amon Shiroshita Taishi Fujimura Yuichiro Shirafuta Ryo Maekawa Toshiaki Taketani Shun Sato Norihiro Sugino Differential gene expression in decidualized human endometrial stromal cells induced by different stimuli Scientific Reports |
| title | Differential gene expression in decidualized human endometrial stromal cells induced by different stimuli |
| title_full | Differential gene expression in decidualized human endometrial stromal cells induced by different stimuli |
| title_fullStr | Differential gene expression in decidualized human endometrial stromal cells induced by different stimuli |
| title_full_unstemmed | Differential gene expression in decidualized human endometrial stromal cells induced by different stimuli |
| title_short | Differential gene expression in decidualized human endometrial stromal cells induced by different stimuli |
| title_sort | differential gene expression in decidualized human endometrial stromal cells induced by different stimuli |
| url | https://doi.org/10.1038/s41598-024-58065-z |
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