Current progress on in vitro differentiation of ovarian follicles from pluripotent stem cells
Mammalian female reproduction requires a functional ovary. Competence of the ovary is determined by the quality of its basic unit–ovarian follicles. A normal follicle consists of an oocyte enclosed within ovarian follicular cells. In humans and mice, the ovarian follicles are formed at the foetal an...
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| Format: | Article |
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Frontiers Media S.A.
2023-06-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2023.1166351/full |
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| author | Genie Min Ju Wu Andy Chun Hang Chen Andy Chun Hang Chen Andy Chun Hang Chen William Shu Biu Yeung William Shu Biu Yeung William Shu Biu Yeung Yin Lau Lee Yin Lau Lee Yin Lau Lee |
| author_facet | Genie Min Ju Wu Andy Chun Hang Chen Andy Chun Hang Chen Andy Chun Hang Chen William Shu Biu Yeung William Shu Biu Yeung William Shu Biu Yeung Yin Lau Lee Yin Lau Lee Yin Lau Lee |
| author_sort | Genie Min Ju Wu |
| collection | DOAJ |
| description | Mammalian female reproduction requires a functional ovary. Competence of the ovary is determined by the quality of its basic unit–ovarian follicles. A normal follicle consists of an oocyte enclosed within ovarian follicular cells. In humans and mice, the ovarian follicles are formed at the foetal and the early neonatal stage respectively, and their renewal at the adult stage is controversial. Extensive research emerges recently to produce ovarian follicles in-vitro from different species. Previous reports demonstrated the differentiation of mouse and human pluripotent stem cells into germline cells, termed primordial germ cell-like cells (PGCLCs). The germ cell-specific gene expressions and epigenetic features including global DNA demethylation and histone modifications of the pluripotent stem cells-derived PGCLCs were extensively characterized. The PGCLCs hold potential for forming ovarian follicles or organoids upon cocultured with ovarian somatic cells. Intriguingly, the oocytes isolated from the organoids could be fertilized in-vitro. Based on the knowledge of in-vivo derived pre-granulosa cells, the generation of these cells from pluripotent stem cells termed foetal ovarian somatic cell-like cells was also reported recently. Despite successful in-vitro folliculogenesis from pluripotent stem cells, the efficiency remains low, mainly due to the lack of information on the interaction between PGCLCs and pre-granulosa cells. The establishment of in-vitro pluripotent stem cell-based models paves the way for understanding the critical signalling pathways and molecules during folliculogenesis. This article aims to review the developmental events during in-vivo follicular development and discuss the current progress of generation of PGCLCs, pre-granulosa and theca cells in-vitro. |
| first_indexed | 2024-03-13T08:06:20Z |
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| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-03-13T08:06:20Z |
| publishDate | 2023-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-6c2100a1a34246e09721399ed9981b4b2023-06-01T04:50:03ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2023-06-011110.3389/fcell.2023.11663511166351Current progress on in vitro differentiation of ovarian follicles from pluripotent stem cellsGenie Min Ju Wu0Andy Chun Hang Chen1Andy Chun Hang Chen2Andy Chun Hang Chen3William Shu Biu Yeung4William Shu Biu Yeung5William Shu Biu Yeung6Yin Lau Lee7Yin Lau Lee8Yin Lau Lee9Department of Obstetrics and Gynaecology, School of Clinical Medicine, The University of Hong Kong, Hong Kong, ChinaDepartment of Obstetrics and Gynaecology, School of Clinical Medicine, The University of Hong Kong, Hong Kong, ChinaShenzhen Key Laboratory of Fertility Regulation, Reproductive Medicine Center, The University of Hong Kong—Shenzhen Hospital, Shenzhen, ChinaCentre for Translational Stem Cell Biology, The Hong Kong Science and Technology Park, Hong Kong, ChinaDepartment of Obstetrics and Gynaecology, School of Clinical Medicine, The University of Hong Kong, Hong Kong, ChinaShenzhen Key Laboratory of Fertility Regulation, Reproductive Medicine Center, The University of Hong Kong—Shenzhen Hospital, Shenzhen, ChinaCentre for Translational Stem Cell Biology, The Hong Kong Science and Technology Park, Hong Kong, ChinaDepartment of Obstetrics and Gynaecology, School of Clinical Medicine, The University of Hong Kong, Hong Kong, ChinaShenzhen Key Laboratory of Fertility Regulation, Reproductive Medicine Center, The University of Hong Kong—Shenzhen Hospital, Shenzhen, ChinaCentre for Translational Stem Cell Biology, The Hong Kong Science and Technology Park, Hong Kong, ChinaMammalian female reproduction requires a functional ovary. Competence of the ovary is determined by the quality of its basic unit–ovarian follicles. A normal follicle consists of an oocyte enclosed within ovarian follicular cells. In humans and mice, the ovarian follicles are formed at the foetal and the early neonatal stage respectively, and their renewal at the adult stage is controversial. Extensive research emerges recently to produce ovarian follicles in-vitro from different species. Previous reports demonstrated the differentiation of mouse and human pluripotent stem cells into germline cells, termed primordial germ cell-like cells (PGCLCs). The germ cell-specific gene expressions and epigenetic features including global DNA demethylation and histone modifications of the pluripotent stem cells-derived PGCLCs were extensively characterized. The PGCLCs hold potential for forming ovarian follicles or organoids upon cocultured with ovarian somatic cells. Intriguingly, the oocytes isolated from the organoids could be fertilized in-vitro. Based on the knowledge of in-vivo derived pre-granulosa cells, the generation of these cells from pluripotent stem cells termed foetal ovarian somatic cell-like cells was also reported recently. Despite successful in-vitro folliculogenesis from pluripotent stem cells, the efficiency remains low, mainly due to the lack of information on the interaction between PGCLCs and pre-granulosa cells. The establishment of in-vitro pluripotent stem cell-based models paves the way for understanding the critical signalling pathways and molecules during folliculogenesis. This article aims to review the developmental events during in-vivo follicular development and discuss the current progress of generation of PGCLCs, pre-granulosa and theca cells in-vitro.https://www.frontiersin.org/articles/10.3389/fcell.2023.1166351/fullprimordial germ cellsgranulosa cellsfollicular developmentin vitro differentiationepigeneticspluripotent stem cells |
| spellingShingle | Genie Min Ju Wu Andy Chun Hang Chen Andy Chun Hang Chen Andy Chun Hang Chen William Shu Biu Yeung William Shu Biu Yeung William Shu Biu Yeung Yin Lau Lee Yin Lau Lee Yin Lau Lee Current progress on in vitro differentiation of ovarian follicles from pluripotent stem cells Frontiers in Cell and Developmental Biology primordial germ cells granulosa cells follicular development in vitro differentiation epigenetics pluripotent stem cells |
| title | Current progress on in vitro differentiation of ovarian follicles from pluripotent stem cells |
| title_full | Current progress on in vitro differentiation of ovarian follicles from pluripotent stem cells |
| title_fullStr | Current progress on in vitro differentiation of ovarian follicles from pluripotent stem cells |
| title_full_unstemmed | Current progress on in vitro differentiation of ovarian follicles from pluripotent stem cells |
| title_short | Current progress on in vitro differentiation of ovarian follicles from pluripotent stem cells |
| title_sort | current progress on in vitro differentiation of ovarian follicles from pluripotent stem cells |
| topic | primordial germ cells granulosa cells follicular development in vitro differentiation epigenetics pluripotent stem cells |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2023.1166351/full |
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