Discharge of embryos from in vitro bovine production: a sustainable vision of the process
Brazil is the world's largest producer of in vitro embryos, so breeders in the country have strong interest in increasing the sustainability of the production process of this biotechnology. In vitro embryo production (IVP) leads to large waste of good quality embryos, so studies that aim to de...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Instituto de Zootecnia
2017-11-01
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| Series: | Boletim de Indústria Animal |
| Subjects: | |
| Online Access: | http://10.153.18.28/index.php/bia/article/view/1824 |
| _version_ | 1797744773782568960 |
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| author | Isabele Picada Emanuelli Danieli Aparecida Bobbo Moreski Ana Carolina Fanhani de Arruda Botelho Pedro Henrique Baeza Burali Francielli Gasparotto Fábio Luiz Bim Cavalieri |
| author_facet | Isabele Picada Emanuelli Danieli Aparecida Bobbo Moreski Ana Carolina Fanhani de Arruda Botelho Pedro Henrique Baeza Burali Francielli Gasparotto Fábio Luiz Bim Cavalieri |
| author_sort | Isabele Picada Emanuelli |
| collection | DOAJ |
| description |
Brazil is the world's largest producer of in vitro embryos, so breeders in the country have strong interest in increasing the sustainability of the production process of this biotechnology. In vitro embryo production (IVP) leads to large waste of good quality embryos, so studies that aim to detect real losses of IVP embryos are important due to the need to develop more sustainable reproductive processes. The objective of this work was to ascertain the disposal rate of viable embryos from the bovine IVP database and present proposals to reduce production losses. The survey was carried out in a laboratory database that contains information about 2,399 aspirations performed between 2013 and 2016. The in vitro embryo production was carried out in the laboratory using processes such as maturation, fertilization and in vitro culture. On the seventh day of culture, the embryos were evaluated for viability for transfer, with some used and others discarded. Data were analyzed using descriptive statistics and showed the following rates of viable embryos and viable embryos wasted, respectively: in 2013 - 29% (2312/7905) and 7% (167/2312); in 2014 - 34.4% (1824/5308) and 11% (195/1824); in 2015 - 37.1% (3100/8355) and 21% (559/3100); and in 2016 - 30.1% (2165/7192) and 32% (530/2165). In four years of studies the total embryo production was 32% (9401/28760), with 15% (1451/9401) waste. This waste occurred because the average production of embryos exceeded the number of available synchronized receivers. Analyzed economically, this practice of discarding viable in vitro embryos generates a lower profit. From an environmental standpoint, this discarding wastes materials and consumes unnecessary energy. Thus, the best solution would be freezing these embryos. However, so far the most limiting factors are the vulnerability to cryopreservation, which exists possibly due to the accumulation of lipids in the cytoplasm. To summarize, the total loss of in vitro embryos was 15%. We propose a plan to count the number of synchronized recipients, taking into account breed, population and donor's history of aspiration (short term resolution). In the long term, a solution would be to establish an efficient embryonic cryopreservation technique.
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| first_indexed | 2024-03-12T15:14:03Z |
| format | Article |
| id | doaj.art-6c2394ef073e4b39b4b071c6e875e8c8 |
| institution | Directory Open Access Journal |
| issn | 1981-4100 |
| language | English |
| last_indexed | 2024-03-12T15:14:03Z |
| publishDate | 2017-11-01 |
| publisher | Instituto de Zootecnia |
| record_format | Article |
| series | Boletim de Indústria Animal |
| spelling | doaj.art-6c2394ef073e4b39b4b071c6e875e8c82023-08-11T17:40:17ZengInstituto de ZootecniaBoletim de Indústria Animal1981-41002017-11-0174Discharge of embryos from in vitro bovine production: a sustainable vision of the processIsabele Picada EmanuelliDanieli Aparecida Bobbo MoreskiAna Carolina Fanhani de Arruda BotelhoPedro Henrique Baeza BuraliFrancielli GasparottoFábio Luiz Bim Cavalieri Brazil is the world's largest producer of in vitro embryos, so breeders in the country have strong interest in increasing the sustainability of the production process of this biotechnology. In vitro embryo production (IVP) leads to large waste of good quality embryos, so studies that aim to detect real losses of IVP embryos are important due to the need to develop more sustainable reproductive processes. The objective of this work was to ascertain the disposal rate of viable embryos from the bovine IVP database and present proposals to reduce production losses. The survey was carried out in a laboratory database that contains information about 2,399 aspirations performed between 2013 and 2016. The in vitro embryo production was carried out in the laboratory using processes such as maturation, fertilization and in vitro culture. On the seventh day of culture, the embryos were evaluated for viability for transfer, with some used and others discarded. Data were analyzed using descriptive statistics and showed the following rates of viable embryos and viable embryos wasted, respectively: in 2013 - 29% (2312/7905) and 7% (167/2312); in 2014 - 34.4% (1824/5308) and 11% (195/1824); in 2015 - 37.1% (3100/8355) and 21% (559/3100); and in 2016 - 30.1% (2165/7192) and 32% (530/2165). In four years of studies the total embryo production was 32% (9401/28760), with 15% (1451/9401) waste. This waste occurred because the average production of embryos exceeded the number of available synchronized receivers. Analyzed economically, this practice of discarding viable in vitro embryos generates a lower profit. From an environmental standpoint, this discarding wastes materials and consumes unnecessary energy. Thus, the best solution would be freezing these embryos. However, so far the most limiting factors are the vulnerability to cryopreservation, which exists possibly due to the accumulation of lipids in the cytoplasm. To summarize, the total loss of in vitro embryos was 15%. We propose a plan to count the number of synchronized recipients, taking into account breed, population and donor's history of aspiration (short term resolution). In the long term, a solution would be to establish an efficient embryonic cryopreservation technique. http://10.153.18.28/index.php/bia/article/view/1824aspirationcryopreservationembryo receptors |
| spellingShingle | Isabele Picada Emanuelli Danieli Aparecida Bobbo Moreski Ana Carolina Fanhani de Arruda Botelho Pedro Henrique Baeza Burali Francielli Gasparotto Fábio Luiz Bim Cavalieri Discharge of embryos from in vitro bovine production: a sustainable vision of the process Boletim de Indústria Animal aspiration cryopreservation embryo receptors |
| title | Discharge of embryos from in vitro bovine production: a sustainable vision of the process |
| title_full | Discharge of embryos from in vitro bovine production: a sustainable vision of the process |
| title_fullStr | Discharge of embryos from in vitro bovine production: a sustainable vision of the process |
| title_full_unstemmed | Discharge of embryos from in vitro bovine production: a sustainable vision of the process |
| title_short | Discharge of embryos from in vitro bovine production: a sustainable vision of the process |
| title_sort | discharge of embryos from in vitro bovine production a sustainable vision of the process |
| topic | aspiration cryopreservation embryo receptors |
| url | http://10.153.18.28/index.php/bia/article/view/1824 |
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