Transferable Extended-Spectrum β-Lactamase (ESBL) Plasmids in <i>Enterobacteriaceae</i> from Irrigation Water
Extended-spectrum β-lactamase (ESBL)-producing <i>Enterobacteriaceae</i> are classified as serious threats to human health by the U.S. Centers for Disease Control and Prevention. Water used for irrigation of fresh produce can transmit such resistant bacteria directly to edible plant part...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-06-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/7/978 |
| _version_ | 1797563721564815360 |
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| author | Maria-Theresia Gekenidis Anita Kläui Kornelia Smalla David Drissner |
| author_facet | Maria-Theresia Gekenidis Anita Kläui Kornelia Smalla David Drissner |
| author_sort | Maria-Theresia Gekenidis |
| collection | DOAJ |
| description | Extended-spectrum β-lactamase (ESBL)-producing <i>Enterobacteriaceae</i> are classified as serious threats to human health by the U.S. Centers for Disease Control and Prevention. Water used for irrigation of fresh produce can transmit such resistant bacteria directly to edible plant parts. We screened ESBL-producing <i>Escherichia coli</i>, <i>Enterobacter cloacae</i>, and <i>Citrobacter freundii</i> isolated from irrigation water for their potential to transmit resistance to antibiotic-susceptible <i>E. coli</i>. All strains were genome-sequenced and tested in vitro for transmission of resistance to third-generation cephalosporins on solid agar as well as in liquid culture. Of the 19 screened isolates, five ESBL-producing <i>E. coli</i> were able to transfer resistance with different efficiency to susceptible recipient <i>E. coli</i>. Transconjugant strains were sequenced for detection of transferred antibiotic resistance genes (ARGs) and compared to the known ARG pattern of their respective donors. Additionally, phenotypic resistance patterns were obtained for both transconjugant and corresponding donor strains, confirming ESBL-producing phenotypes of all obtained transconjugants. |
| first_indexed | 2024-03-10T18:47:27Z |
| format | Article |
| id | doaj.art-6c2ad252eeed4f3295d68c123fc56940 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T18:47:27Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-6c2ad252eeed4f3295d68c123fc569402023-11-20T05:23:08ZengMDPI AGMicroorganisms2076-26072020-06-018797810.3390/microorganisms8070978Transferable Extended-Spectrum β-Lactamase (ESBL) Plasmids in <i>Enterobacteriaceae</i> from Irrigation WaterMaria-Theresia Gekenidis0Anita Kläui1Kornelia Smalla2David Drissner3Microbiological Food Safety, Agroscope, 8820 Wädenswil, SwitzerlandMicrobiological Food Safety, Agroscope, 3003 Liebefeld, SwitzerlandInstitute for Epidemiology and Pathogen Diagnostics, Julius Kühn-Institut—Federal Research Centre for Cultivated Plants (JKI), 38104 Braunschweig, GermanyDepartment of Life Sciences, Albstadt-Sigmaringen University, 72488 Sigmaringen, GermanyExtended-spectrum β-lactamase (ESBL)-producing <i>Enterobacteriaceae</i> are classified as serious threats to human health by the U.S. Centers for Disease Control and Prevention. Water used for irrigation of fresh produce can transmit such resistant bacteria directly to edible plant parts. We screened ESBL-producing <i>Escherichia coli</i>, <i>Enterobacter cloacae</i>, and <i>Citrobacter freundii</i> isolated from irrigation water for their potential to transmit resistance to antibiotic-susceptible <i>E. coli</i>. All strains were genome-sequenced and tested in vitro for transmission of resistance to third-generation cephalosporins on solid agar as well as in liquid culture. Of the 19 screened isolates, five ESBL-producing <i>E. coli</i> were able to transfer resistance with different efficiency to susceptible recipient <i>E. coli</i>. Transconjugant strains were sequenced for detection of transferred antibiotic resistance genes (ARGs) and compared to the known ARG pattern of their respective donors. Additionally, phenotypic resistance patterns were obtained for both transconjugant and corresponding donor strains, confirming ESBL-producing phenotypes of all obtained transconjugants.https://www.mdpi.com/2076-2607/8/7/978irrigation waterantibiotic resistanceESBL <i>Enterobacteriaceae</i>conjugative plasmids |
| spellingShingle | Maria-Theresia Gekenidis Anita Kläui Kornelia Smalla David Drissner Transferable Extended-Spectrum β-Lactamase (ESBL) Plasmids in <i>Enterobacteriaceae</i> from Irrigation Water Microorganisms irrigation water antibiotic resistance ESBL <i>Enterobacteriaceae</i> conjugative plasmids |
| title | Transferable Extended-Spectrum β-Lactamase (ESBL) Plasmids in <i>Enterobacteriaceae</i> from Irrigation Water |
| title_full | Transferable Extended-Spectrum β-Lactamase (ESBL) Plasmids in <i>Enterobacteriaceae</i> from Irrigation Water |
| title_fullStr | Transferable Extended-Spectrum β-Lactamase (ESBL) Plasmids in <i>Enterobacteriaceae</i> from Irrigation Water |
| title_full_unstemmed | Transferable Extended-Spectrum β-Lactamase (ESBL) Plasmids in <i>Enterobacteriaceae</i> from Irrigation Water |
| title_short | Transferable Extended-Spectrum β-Lactamase (ESBL) Plasmids in <i>Enterobacteriaceae</i> from Irrigation Water |
| title_sort | transferable extended spectrum β lactamase esbl plasmids in i enterobacteriaceae i from irrigation water |
| topic | irrigation water antibiotic resistance ESBL <i>Enterobacteriaceae</i> conjugative plasmids |
| url | https://www.mdpi.com/2076-2607/8/7/978 |
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