Enzymatic Kinetics of Solvent-free Esterification with Bio-imprinted Lipase
To avoid the use of compounds that burden the environment, a solvent-free enzymatic reaction was the focus of this study. Investigated were the catalytic activities and kinetics of lipases that were pretreated with carboxylic acids for the solvent-free esterification of propionic acid with isoamyl a...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Croatian Society of Chemical Engineers
2020-01-01
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| Series: | Chemical and Biochemical Engineering Quarterly |
| Subjects: | |
| Online Access: | http://silverstripe.fkit.hr/cabeq/assets/Uploads/07-4-19.pdf |
| _version_ | 1818189765380407296 |
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| author | M. Matsumoto Y. Hasegawa |
| author_facet | M. Matsumoto Y. Hasegawa |
| author_sort | M. Matsumoto |
| collection | DOAJ |
| description | To avoid the use of compounds that burden the environment, a solvent-free enzymatic reaction was the focus of this study. Investigated were the catalytic activities and kinetics of lipases that were pretreated with carboxylic acids for the solvent-free esterification of propionic acid with isoamyl alcohol. The enhancements of the esterification yields and rates by the bio-imprinting effects of carboxylic acids were observed. We found no inhibition of isoamyl alcohol on the solvent-free enzymatic esterification, and obtained a large imprinting effect under a largely excessive amount of isoamyl alcohol to propionic acid. From the kinetic analysis, the imprinting of lipases mainly enhanced the catalytic reaction rate constant rather than the affinity between lipase and propionic acid compared with untreated lipase. The bio-imprinting treatment of lipase is found to be very effective for the yield and kinetics in solvent-free esterification. |
| first_indexed | 2024-12-11T23:48:01Z |
| format | Article |
| id | doaj.art-6c2efa8f462b4e71b47dce3460f650ba |
| institution | Directory Open Access Journal |
| issn | 0352-9568 1846-5153 |
| language | English |
| last_indexed | 2024-12-11T23:48:01Z |
| publishDate | 2020-01-01 |
| publisher | Croatian Society of Chemical Engineers |
| record_format | Article |
| series | Chemical and Biochemical Engineering Quarterly |
| spelling | doaj.art-6c2efa8f462b4e71b47dce3460f650ba2022-12-22T00:45:33ZengCroatian Society of Chemical EngineersChemical and Biochemical Engineering Quarterly0352-95681846-51532020-01-0133449549910.15255/CABEQ.2019.1692Enzymatic Kinetics of Solvent-free Esterification with Bio-imprinted LipaseM. Matsumoto0Y. Hasegawa1Department of Chemical Engineering and Materials Science, Doshisha University, Kyotanabe, Kyoto 610-0321, JapanDepartment of Chemical Engineering and Materials Science, Doshisha University, Kyotanabe, Kyoto 610-0321, JapanTo avoid the use of compounds that burden the environment, a solvent-free enzymatic reaction was the focus of this study. Investigated were the catalytic activities and kinetics of lipases that were pretreated with carboxylic acids for the solvent-free esterification of propionic acid with isoamyl alcohol. The enhancements of the esterification yields and rates by the bio-imprinting effects of carboxylic acids were observed. We found no inhibition of isoamyl alcohol on the solvent-free enzymatic esterification, and obtained a large imprinting effect under a largely excessive amount of isoamyl alcohol to propionic acid. From the kinetic analysis, the imprinting of lipases mainly enhanced the catalytic reaction rate constant rather than the affinity between lipase and propionic acid compared with untreated lipase. The bio-imprinting treatment of lipase is found to be very effective for the yield and kinetics in solvent-free esterification.http://silverstripe.fkit.hr/cabeq/assets/Uploads/07-4-19.pdflipasebio-imprintingsolvent-free esterification |
| spellingShingle | M. Matsumoto Y. Hasegawa Enzymatic Kinetics of Solvent-free Esterification with Bio-imprinted Lipase Chemical and Biochemical Engineering Quarterly lipase bio-imprinting solvent-free esterification |
| title | Enzymatic Kinetics of Solvent-free Esterification with Bio-imprinted Lipase |
| title_full | Enzymatic Kinetics of Solvent-free Esterification with Bio-imprinted Lipase |
| title_fullStr | Enzymatic Kinetics of Solvent-free Esterification with Bio-imprinted Lipase |
| title_full_unstemmed | Enzymatic Kinetics of Solvent-free Esterification with Bio-imprinted Lipase |
| title_short | Enzymatic Kinetics of Solvent-free Esterification with Bio-imprinted Lipase |
| title_sort | enzymatic kinetics of solvent free esterification with bio imprinted lipase |
| topic | lipase bio-imprinting solvent-free esterification |
| url | http://silverstripe.fkit.hr/cabeq/assets/Uploads/07-4-19.pdf |
| work_keys_str_mv | AT mmatsumoto enzymatickineticsofsolventfreeesterificationwithbioimprintedlipase AT yhasegawa enzymatickineticsofsolventfreeesterificationwithbioimprintedlipase |