Transcriptome Analysis Provides Insights into Copulation, Fertilization, and Gestation in <i>Sebastes schlegelii</i>
Among the viviparous marine teleosts of China, the black rockfish (<i>Sebastes schlegelii</i> Hilgendorf) is one of the most economically important. In addition to copulation and internal fertilization, it features lengthy sperm storage in the female ovary as well as a high rate of abort...
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| Format: | Article |
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MDPI AG
2022-10-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/13/10/1812 |
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| author | Xueying Wang Ning Zhao Tao Wang Shuran Du Qinghua Liu Jun Li |
| author_facet | Xueying Wang Ning Zhao Tao Wang Shuran Du Qinghua Liu Jun Li |
| author_sort | Xueying Wang |
| collection | DOAJ |
| description | Among the viviparous marine teleosts of China, the black rockfish (<i>Sebastes schlegelii</i> Hilgendorf) is one of the most economically important. In addition to copulation and internal fertilization, it features lengthy sperm storage in the female ovary as well as a high rate of abortion. A network of gene regulation is necessary for these processes. To elucidate the mechanisms of copulation, fertilization, and gestation, it is essential to determine the genetic basis of viviparous teleost oogenesis and embryogenesis. In this study, we analyzed the transcriptome of the ovary during different developmental phases to investigate the dynamic changes that occur. We constructed 24 ovary transcriptomes. In order to investigate the regulation of embryogenesis, differentially expressed genes (DEGs) with specific expression patterns were subjected to gene ontology annotation, pathway analyses, and weighted gene co-expression network analysis (WGCNA). The up-regulated genes were significantly enriched in focal adhesion, regulation of the actin cytoskeleton, Wnt, and ECM-receptor interaction signaling pathways. As a result of our study, we provide omics evidence for copulation, fertilization, and gestation in viviparous marine teleosts. Decoding the <i>S. schlegelii</i> gene regulation network, as well as providing new insights into embryogenesis, is highly valuable to researchers in the marine teleost reproduction sciences. |
| first_indexed | 2024-03-09T20:10:41Z |
| format | Article |
| id | doaj.art-6c3534c805be40f6a78e8b4949efad78 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-09T20:10:41Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-6c3534c805be40f6a78e8b4949efad782023-11-24T00:15:48ZengMDPI AGGenes2073-44252022-10-011310181210.3390/genes13101812Transcriptome Analysis Provides Insights into Copulation, Fertilization, and Gestation in <i>Sebastes schlegelii</i>Xueying Wang0Ning Zhao1Tao Wang2Shuran Du3Qinghua Liu4Jun Li5CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaCAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaCAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaCAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaCAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaCAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaAmong the viviparous marine teleosts of China, the black rockfish (<i>Sebastes schlegelii</i> Hilgendorf) is one of the most economically important. In addition to copulation and internal fertilization, it features lengthy sperm storage in the female ovary as well as a high rate of abortion. A network of gene regulation is necessary for these processes. To elucidate the mechanisms of copulation, fertilization, and gestation, it is essential to determine the genetic basis of viviparous teleost oogenesis and embryogenesis. In this study, we analyzed the transcriptome of the ovary during different developmental phases to investigate the dynamic changes that occur. We constructed 24 ovary transcriptomes. In order to investigate the regulation of embryogenesis, differentially expressed genes (DEGs) with specific expression patterns were subjected to gene ontology annotation, pathway analyses, and weighted gene co-expression network analysis (WGCNA). The up-regulated genes were significantly enriched in focal adhesion, regulation of the actin cytoskeleton, Wnt, and ECM-receptor interaction signaling pathways. As a result of our study, we provide omics evidence for copulation, fertilization, and gestation in viviparous marine teleosts. Decoding the <i>S. schlegelii</i> gene regulation network, as well as providing new insights into embryogenesis, is highly valuable to researchers in the marine teleost reproduction sciences.https://www.mdpi.com/2073-4425/13/10/1812<i>S. schlegelii</i>copulationfertilizationembryogenesisRNA-seq |
| spellingShingle | Xueying Wang Ning Zhao Tao Wang Shuran Du Qinghua Liu Jun Li Transcriptome Analysis Provides Insights into Copulation, Fertilization, and Gestation in <i>Sebastes schlegelii</i> Genes <i>S. schlegelii</i> copulation fertilization embryogenesis RNA-seq |
| title | Transcriptome Analysis Provides Insights into Copulation, Fertilization, and Gestation in <i>Sebastes schlegelii</i> |
| title_full | Transcriptome Analysis Provides Insights into Copulation, Fertilization, and Gestation in <i>Sebastes schlegelii</i> |
| title_fullStr | Transcriptome Analysis Provides Insights into Copulation, Fertilization, and Gestation in <i>Sebastes schlegelii</i> |
| title_full_unstemmed | Transcriptome Analysis Provides Insights into Copulation, Fertilization, and Gestation in <i>Sebastes schlegelii</i> |
| title_short | Transcriptome Analysis Provides Insights into Copulation, Fertilization, and Gestation in <i>Sebastes schlegelii</i> |
| title_sort | transcriptome analysis provides insights into copulation fertilization and gestation in i sebastes schlegelii i |
| topic | <i>S. schlegelii</i> copulation fertilization embryogenesis RNA-seq |
| url | https://www.mdpi.com/2073-4425/13/10/1812 |
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