Targeted detection of the cancer cells using the anti-CD24 bio modified PEGylated gold nanoparticles: the application of CD24 as a vital cancer biomarker
Objective(s): The central role of molecular imaging modalities in cancer management is an undeniable fact that could help to diagnose cancer tumors in early stages. The main aim of this study is to prepare a novel targeted molecular imaging nanoprobe of CD24-PEGylated Au NPs to improve the ability o...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2018-07-01
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| Series: | Nanomedicine Journal |
| Subjects: | |
| Online Access: | http://nmj.mums.ac.ir/article_11164_2335f41ac7c06c02ae8f40db8736d69e.pdf |
| _version_ | 1828426642767216640 |
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| author | Mona Fazel-Ghaziyani Daryoush Shahbazi-Gahrouei Mohammad Pourhassan-Moghaddam Behzad Baradaran Mostafa Ghavami |
| author_facet | Mona Fazel-Ghaziyani Daryoush Shahbazi-Gahrouei Mohammad Pourhassan-Moghaddam Behzad Baradaran Mostafa Ghavami |
| author_sort | Mona Fazel-Ghaziyani |
| collection | DOAJ |
| description | Objective(s): The central role of molecular imaging modalities in cancer management is an undeniable fact that could help to diagnose cancer tumors in early stages. The main aim of this study is to prepare a novel targeted molecular imaging nanoprobe of CD24-PEGylated Au NPs to improve the ability of Computed tomography scanning (CT scan) outputs for both in vitro and in vivo detection of breast cancer (4T1) cells. Materials and methods: Gold nanoparticles (Au NPs) were synthesized and coated with polyethylene glycol (PEG) chains in order to improve the stability of the Au NPs and to provide bio modification points for antibody immobilization. The synthesized nanoprobe was used for both in vitro and in vivo targeted CT imaging breast cancer cells (4T1) and the xenograft tumor model. Results: Findings showed that the attenuation coefficient of 4T1 cells that were targeted by CD24-PEGylated Au NPs is calculated to be over two times higher than the untargeted 4T1 cells (15 HU vs 39 HU, respectively). Indeed, the results clearly reveal that the developed CD24-PEGylated Au NPs showed the tumor CT enhancement was higher than that of Omnipaqe which used as control. Also, the CT number values of the tumor area at different time points gradually increased after 60 min post injection and was significantly higher than before injection. Conclusions: Results showed the introduced CT imaging nanoprobe (Au NPs-PEGylated) could be useful for CT imaging of breast tumors under in vivo condition. Overall, it is concluded that Au NPs-PEGylated contrast media is able to detect breast cancer (4T1) cells and is more effective compared with other casual methods. |
| first_indexed | 2024-12-10T16:49:13Z |
| format | Article |
| id | doaj.art-6c359480d8424ac2852c24b60c5e6d12 |
| institution | Directory Open Access Journal |
| issn | 2322-3049 2322-5904 |
| language | English |
| last_indexed | 2024-12-10T16:49:13Z |
| publishDate | 2018-07-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Nanomedicine Journal |
| spelling | doaj.art-6c359480d8424ac2852c24b60c5e6d122022-12-22T01:40:56ZengMashhad University of Medical SciencesNanomedicine Journal2322-30492322-59042018-07-015317217910.22038/nmj.2018.005.000711164Targeted detection of the cancer cells using the anti-CD24 bio modified PEGylated gold nanoparticles: the application of CD24 as a vital cancer biomarkerMona Fazel-Ghaziyani0Daryoush Shahbazi-Gahrouei1Mohammad Pourhassan-Moghaddam2Behzad Baradaran3Mostafa Ghavami4Department of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran|Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, IranImmunology Research Center, Tabriz University of Medical Sciences, Tabriz, IranDepartment of Radiology, Paramedical School, Tabriz University of Medical Sciences, Tabriz, IranObjective(s): The central role of molecular imaging modalities in cancer management is an undeniable fact that could help to diagnose cancer tumors in early stages. The main aim of this study is to prepare a novel targeted molecular imaging nanoprobe of CD24-PEGylated Au NPs to improve the ability of Computed tomography scanning (CT scan) outputs for both in vitro and in vivo detection of breast cancer (4T1) cells. Materials and methods: Gold nanoparticles (Au NPs) were synthesized and coated with polyethylene glycol (PEG) chains in order to improve the stability of the Au NPs and to provide bio modification points for antibody immobilization. The synthesized nanoprobe was used for both in vitro and in vivo targeted CT imaging breast cancer cells (4T1) and the xenograft tumor model. Results: Findings showed that the attenuation coefficient of 4T1 cells that were targeted by CD24-PEGylated Au NPs is calculated to be over two times higher than the untargeted 4T1 cells (15 HU vs 39 HU, respectively). Indeed, the results clearly reveal that the developed CD24-PEGylated Au NPs showed the tumor CT enhancement was higher than that of Omnipaqe which used as control. Also, the CT number values of the tumor area at different time points gradually increased after 60 min post injection and was significantly higher than before injection. Conclusions: Results showed the introduced CT imaging nanoprobe (Au NPs-PEGylated) could be useful for CT imaging of breast tumors under in vivo condition. Overall, it is concluded that Au NPs-PEGylated contrast media is able to detect breast cancer (4T1) cells and is more effective compared with other casual methods.http://nmj.mums.ac.ir/article_11164_2335f41ac7c06c02ae8f40db8736d69e.pdfGold nanoparticlePolyethylene glycol (PEG)CD24 antibodyComputed tomography4T1Cancer detection |
| spellingShingle | Mona Fazel-Ghaziyani Daryoush Shahbazi-Gahrouei Mohammad Pourhassan-Moghaddam Behzad Baradaran Mostafa Ghavami Targeted detection of the cancer cells using the anti-CD24 bio modified PEGylated gold nanoparticles: the application of CD24 as a vital cancer biomarker Nanomedicine Journal Gold nanoparticle Polyethylene glycol (PEG) CD24 antibody Computed tomography 4T1 Cancer detection |
| title | Targeted detection of the cancer cells using the anti-CD24 bio modified PEGylated gold nanoparticles: the application of CD24 as a vital cancer biomarker |
| title_full | Targeted detection of the cancer cells using the anti-CD24 bio modified PEGylated gold nanoparticles: the application of CD24 as a vital cancer biomarker |
| title_fullStr | Targeted detection of the cancer cells using the anti-CD24 bio modified PEGylated gold nanoparticles: the application of CD24 as a vital cancer biomarker |
| title_full_unstemmed | Targeted detection of the cancer cells using the anti-CD24 bio modified PEGylated gold nanoparticles: the application of CD24 as a vital cancer biomarker |
| title_short | Targeted detection of the cancer cells using the anti-CD24 bio modified PEGylated gold nanoparticles: the application of CD24 as a vital cancer biomarker |
| title_sort | targeted detection of the cancer cells using the anti cd24 bio modified pegylated gold nanoparticles the application of cd24 as a vital cancer biomarker |
| topic | Gold nanoparticle Polyethylene glycol (PEG) CD24 antibody Computed tomography 4T1 Cancer detection |
| url | http://nmj.mums.ac.ir/article_11164_2335f41ac7c06c02ae8f40db8736d69e.pdf |
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