HER-2 Expression in Colorectal Cancer and Its Correlation with Immune Cell Infiltration
Background: This study aimed to investigate the effect of increased HER-2 expression on tumor-infiltrating lymphocytes (TILs) and determine its impact on the prognosis of colorectal cancer (CRC) patients; Methods: HER-2, CD4, CD8, CD19, LY6G, CD56, CD68, CD11b, and EpCam expression in CRC tissues an...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-10-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/11/11/2889 |
| _version_ | 1797460046946238464 |
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| author | Di Yang Bo Wang Yinuo Li Jingyao Zhang Xuantong Gong Hao Qin Yan Wang Yahui Zhao Yong Wang |
| author_facet | Di Yang Bo Wang Yinuo Li Jingyao Zhang Xuantong Gong Hao Qin Yan Wang Yahui Zhao Yong Wang |
| author_sort | Di Yang |
| collection | DOAJ |
| description | Background: This study aimed to investigate the effect of increased HER-2 expression on tumor-infiltrating lymphocytes (TILs) and determine its impact on the prognosis of colorectal cancer (CRC) patients; Methods: HER-2, CD4, CD8, CD19, LY6G, CD56, CD68, CD11b, and EpCam expression in CRC tissues and adjacent paracancerous tissues were assessed using multiplex fluorescence immunohistochemical staining. The correlation between HER-2 expression and the number of TILs in CRC tissues was analyzed. Kaplan–Meier and Cox proportional hazards models were used to analyze survival outcomes; Results: The expression of HER-2 in tumor tissues was higher than that in paracancerous tissues (1.31 ± 0.45 vs. 0.86 ± 0.20, <i>p</i> < 0.05). Additionally, there was an increase in the numbers of CD4+, CD8+, CD19+, and CD68+ cells in CRC tissues (14.11 ± 1.10 vs. 3.40 ± 0.18, <i>p</i> < 0.005; 0.16 ± 0.12 vs. 0.04 ± 0.04, <i>p</i> < 0.005; 0.71 ± 0.46 vs. 0.25 ± 0.13, <i>p</i> < 0.0005; 0.27 ± 0.24 vs. 0.03 ± 0.11, <i>p</i> < 0.05). An increase in HER-2 expression was positively correlated with an increase in CD4, CD8, and CD19 (<i>p</i> < 0.0001). In HER-2-positive CRC tissues, CD68 expression was increased (0.80 ± 0.55 vs. 0.25 ± 0.22, <i>p</i> < 0.05). In HER-2-upregulated CRC tissues, CD4, CD8, CD19, CD68, CD11b, Ly6G, and CD56 expressions were elevated (0.70 ± 0.37 vs. 0.32 ± 0.17, <i>p</i> = 0.03; 0.22 ± 0.13 vs. 0.09 ± 0.06, <i>p</i> = 0.03; 0.31 ± 0.19 vs. 0.12 ± 0.08, <i>p</i> = 0.02; 1.05 ± 0.62 vs. 0.43 ± 0.21, <i>p</i> < 0.01; 1.34 ± 0.81 vs. 0.53 ± 0.23, <i>p</i> < 0.01; 0.50 ± 0.31 vs. 0.19 ± 0.10, <i>p</i> < 0.01; 1.26 ± 0.74 vs. 0.52 ± 0.24, <i>p</i> < 0.01). Furthermore, increased HER-2 expression was an independent risk factor for recurrence-free survival (RFS) in patients (<i>p</i> < 0.01, HR = 3.421); Conclusions: The increased expression of HER-2 and its relationship with immune cells will provide new insights for immunotherapy in CRC patients. |
| first_indexed | 2024-03-09T17:00:37Z |
| format | Article |
| id | doaj.art-6c369b00ee3043c5a0f369fcce237098 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-09T17:00:37Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-6c369b00ee3043c5a0f369fcce2370982023-11-24T14:30:36ZengMDPI AGBiomedicines2227-90592023-10-011111288910.3390/biomedicines11112889HER-2 Expression in Colorectal Cancer and Its Correlation with Immune Cell InfiltrationDi Yang0Bo Wang1Yinuo Li2Jingyao Zhang3Xuantong Gong4Hao Qin5Yan Wang6Yahui Zhao7Yong Wang8Department of Ultrasound, National Cancer Center, National Clinical Research Center for Cancer, Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, ChinaDepartment of Ultrasound, National Cancer Center, National Clinical Research Center for Cancer, Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, ChinaState Key Laboratory of Molecular Oncology, National Cancer Center, National Clinical Research Center for Cancer, Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, ChinaKey Laboratory of Cancer and Microbiome, State Key Laboratory of Molecular Oncology, National Cancer Center, National Clinical Research Center for Cancer, Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, ChinaDepartment of Ultrasound, National Cancer Center, National Clinical Research Center for Cancer, Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, ChinaKey Laboratory of Cancer and Microbiome, State Key Laboratory of Molecular Oncology, National Cancer Center, National Clinical Research Center for Cancer, Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, ChinaKey Laboratory of Cancer and Microbiome, State Key Laboratory of Molecular Oncology, National Cancer Center, National Clinical Research Center for Cancer, Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, ChinaState Key Laboratory of Molecular Oncology, National Cancer Center, National Clinical Research Center for Cancer, Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, ChinaDepartment of Ultrasound, National Cancer Center, National Clinical Research Center for Cancer, Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, ChinaBackground: This study aimed to investigate the effect of increased HER-2 expression on tumor-infiltrating lymphocytes (TILs) and determine its impact on the prognosis of colorectal cancer (CRC) patients; Methods: HER-2, CD4, CD8, CD19, LY6G, CD56, CD68, CD11b, and EpCam expression in CRC tissues and adjacent paracancerous tissues were assessed using multiplex fluorescence immunohistochemical staining. The correlation between HER-2 expression and the number of TILs in CRC tissues was analyzed. Kaplan–Meier and Cox proportional hazards models were used to analyze survival outcomes; Results: The expression of HER-2 in tumor tissues was higher than that in paracancerous tissues (1.31 ± 0.45 vs. 0.86 ± 0.20, <i>p</i> < 0.05). Additionally, there was an increase in the numbers of CD4+, CD8+, CD19+, and CD68+ cells in CRC tissues (14.11 ± 1.10 vs. 3.40 ± 0.18, <i>p</i> < 0.005; 0.16 ± 0.12 vs. 0.04 ± 0.04, <i>p</i> < 0.005; 0.71 ± 0.46 vs. 0.25 ± 0.13, <i>p</i> < 0.0005; 0.27 ± 0.24 vs. 0.03 ± 0.11, <i>p</i> < 0.05). An increase in HER-2 expression was positively correlated with an increase in CD4, CD8, and CD19 (<i>p</i> < 0.0001). In HER-2-positive CRC tissues, CD68 expression was increased (0.80 ± 0.55 vs. 0.25 ± 0.22, <i>p</i> < 0.05). In HER-2-upregulated CRC tissues, CD4, CD8, CD19, CD68, CD11b, Ly6G, and CD56 expressions were elevated (0.70 ± 0.37 vs. 0.32 ± 0.17, <i>p</i> = 0.03; 0.22 ± 0.13 vs. 0.09 ± 0.06, <i>p</i> = 0.03; 0.31 ± 0.19 vs. 0.12 ± 0.08, <i>p</i> = 0.02; 1.05 ± 0.62 vs. 0.43 ± 0.21, <i>p</i> < 0.01; 1.34 ± 0.81 vs. 0.53 ± 0.23, <i>p</i> < 0.01; 0.50 ± 0.31 vs. 0.19 ± 0.10, <i>p</i> < 0.01; 1.26 ± 0.74 vs. 0.52 ± 0.24, <i>p</i> < 0.01). Furthermore, increased HER-2 expression was an independent risk factor for recurrence-free survival (RFS) in patients (<i>p</i> < 0.01, HR = 3.421); Conclusions: The increased expression of HER-2 and its relationship with immune cells will provide new insights for immunotherapy in CRC patients.https://www.mdpi.com/2227-9059/11/11/2889HER2colorectal cancerimmune cell |
| spellingShingle | Di Yang Bo Wang Yinuo Li Jingyao Zhang Xuantong Gong Hao Qin Yan Wang Yahui Zhao Yong Wang HER-2 Expression in Colorectal Cancer and Its Correlation with Immune Cell Infiltration Biomedicines HER2 colorectal cancer immune cell |
| title | HER-2 Expression in Colorectal Cancer and Its Correlation with Immune Cell Infiltration |
| title_full | HER-2 Expression in Colorectal Cancer and Its Correlation with Immune Cell Infiltration |
| title_fullStr | HER-2 Expression in Colorectal Cancer and Its Correlation with Immune Cell Infiltration |
| title_full_unstemmed | HER-2 Expression in Colorectal Cancer and Its Correlation with Immune Cell Infiltration |
| title_short | HER-2 Expression in Colorectal Cancer and Its Correlation with Immune Cell Infiltration |
| title_sort | her 2 expression in colorectal cancer and its correlation with immune cell infiltration |
| topic | HER2 colorectal cancer immune cell |
| url | https://www.mdpi.com/2227-9059/11/11/2889 |
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