Strawberry Notch 1 (SBNO1) promotes proliferation of spermatogonial stem cells via the noncanonical Wnt pathway in mice
While it is known that spermatogonial stem cells (SSCs) initiate the production of male germ cells, the mechanisms of SSC self-renewal, proliferation, and differentiation remain poorly understood. We have previously identified Strawberry Notch 1 (SBNO1), a vertebrate strawberry notch family protein,...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2019-01-01
|
| Series: | Asian Journal of Andrology |
| Subjects: | |
| Online Access: | http://www.ajandrology.com/article.asp?issn=1008-682X;year=2019;volume=21;issue=4;spage=345;epage=350;aulast=Shen |
| _version_ | 1818192241587388416 |
|---|---|
| author | Cong Shen Jun Yu Xi Zhang Chen-Chen Liu Yue-Shuai Guo Jia-Wei Zhu Ke Zhang Yi Yu Ting-Ting Gao Shen-Min Yang Hong Li Bo Zheng Xiao-Yan Huang |
| author_facet | Cong Shen Jun Yu Xi Zhang Chen-Chen Liu Yue-Shuai Guo Jia-Wei Zhu Ke Zhang Yi Yu Ting-Ting Gao Shen-Min Yang Hong Li Bo Zheng Xiao-Yan Huang |
| author_sort | Cong Shen |
| collection | DOAJ |
| description | While it is known that spermatogonial stem cells (SSCs) initiate the production of male germ cells, the mechanisms of SSC self-renewal, proliferation, and differentiation remain poorly understood. We have previously identified Strawberry Notch 1 (SBNO1), a vertebrate strawberry notch family protein, in the proteome profile for mouse SSC maturation and differentiation, revealing SBNO1 is associated with neonatal testicular development. To explore further the location and function of SBNO1 in the testes, we performed Sbno1 gene knockdown in mice to study the effects of SBNO1 on neonatal testicular and SSC development. Our results revealed that SBNO1 is required for neonatal testicular and SSC development in mice. Particularly, in vitro Sbno1 gene knockdown with morpholino oligonucleotides caused a reduction of SSCs and inactivation of the noncanonical Wnt pathway, through Jun N-terminal kinases. Our study suggests SBNO1 maintains SSCs by promoting the noncanonical Wnt pathway. |
| first_indexed | 2024-12-12T00:27:23Z |
| format | Article |
| id | doaj.art-6c375fa829334ebbaeafb23ffee4249c |
| institution | Directory Open Access Journal |
| issn | 1008-682X 1745-7262 |
| language | English |
| last_indexed | 2024-12-12T00:27:23Z |
| publishDate | 2019-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Journal of Andrology |
| spelling | doaj.art-6c375fa829334ebbaeafb23ffee4249c2022-12-22T00:44:34ZengWolters Kluwer Medknow PublicationsAsian Journal of Andrology1008-682X1745-72622019-01-0121434535010.4103/aja.aja_65_18Strawberry Notch 1 (SBNO1) promotes proliferation of spermatogonial stem cells via the noncanonical Wnt pathway in miceCong ShenJun YuXi ZhangChen-Chen LiuYue-Shuai GuoJia-Wei ZhuKe ZhangYi YuTing-Ting GaoShen-Min YangHong LiBo ZhengXiao-Yan HuangWhile it is known that spermatogonial stem cells (SSCs) initiate the production of male germ cells, the mechanisms of SSC self-renewal, proliferation, and differentiation remain poorly understood. We have previously identified Strawberry Notch 1 (SBNO1), a vertebrate strawberry notch family protein, in the proteome profile for mouse SSC maturation and differentiation, revealing SBNO1 is associated with neonatal testicular development. To explore further the location and function of SBNO1 in the testes, we performed Sbno1 gene knockdown in mice to study the effects of SBNO1 on neonatal testicular and SSC development. Our results revealed that SBNO1 is required for neonatal testicular and SSC development in mice. Particularly, in vitro Sbno1 gene knockdown with morpholino oligonucleotides caused a reduction of SSCs and inactivation of the noncanonical Wnt pathway, through Jun N-terminal kinases. Our study suggests SBNO1 maintains SSCs by promoting the noncanonical Wnt pathway.http://www.ajandrology.com/article.asp?issn=1008-682X;year=2019;volume=21;issue=4;spage=345;epage=350;aulast=Shennoncanonical Wnt pathway; spermatogonial stem cells; Strawberry Notch 1 (SBNO1) |
| spellingShingle | Cong Shen Jun Yu Xi Zhang Chen-Chen Liu Yue-Shuai Guo Jia-Wei Zhu Ke Zhang Yi Yu Ting-Ting Gao Shen-Min Yang Hong Li Bo Zheng Xiao-Yan Huang Strawberry Notch 1 (SBNO1) promotes proliferation of spermatogonial stem cells via the noncanonical Wnt pathway in mice Asian Journal of Andrology noncanonical Wnt pathway; spermatogonial stem cells; Strawberry Notch 1 (SBNO1) |
| title | Strawberry Notch 1 (SBNO1) promotes proliferation of spermatogonial stem cells via the noncanonical Wnt pathway in mice |
| title_full | Strawberry Notch 1 (SBNO1) promotes proliferation of spermatogonial stem cells via the noncanonical Wnt pathway in mice |
| title_fullStr | Strawberry Notch 1 (SBNO1) promotes proliferation of spermatogonial stem cells via the noncanonical Wnt pathway in mice |
| title_full_unstemmed | Strawberry Notch 1 (SBNO1) promotes proliferation of spermatogonial stem cells via the noncanonical Wnt pathway in mice |
| title_short | Strawberry Notch 1 (SBNO1) promotes proliferation of spermatogonial stem cells via the noncanonical Wnt pathway in mice |
| title_sort | strawberry notch 1 sbno1 promotes proliferation of spermatogonial stem cells via the noncanonical wnt pathway in mice |
| topic | noncanonical Wnt pathway; spermatogonial stem cells; Strawberry Notch 1 (SBNO1) |
| url | http://www.ajandrology.com/article.asp?issn=1008-682X;year=2019;volume=21;issue=4;spage=345;epage=350;aulast=Shen |
| work_keys_str_mv | AT congshen strawberrynotch1sbno1promotesproliferationofspermatogonialstemcellsviathenoncanonicalwntpathwayinmice AT junyu strawberrynotch1sbno1promotesproliferationofspermatogonialstemcellsviathenoncanonicalwntpathwayinmice AT xizhang strawberrynotch1sbno1promotesproliferationofspermatogonialstemcellsviathenoncanonicalwntpathwayinmice AT chenchenliu strawberrynotch1sbno1promotesproliferationofspermatogonialstemcellsviathenoncanonicalwntpathwayinmice AT yueshuaiguo strawberrynotch1sbno1promotesproliferationofspermatogonialstemcellsviathenoncanonicalwntpathwayinmice AT jiaweizhu strawberrynotch1sbno1promotesproliferationofspermatogonialstemcellsviathenoncanonicalwntpathwayinmice AT kezhang strawberrynotch1sbno1promotesproliferationofspermatogonialstemcellsviathenoncanonicalwntpathwayinmice AT yiyu strawberrynotch1sbno1promotesproliferationofspermatogonialstemcellsviathenoncanonicalwntpathwayinmice AT tingtinggao strawberrynotch1sbno1promotesproliferationofspermatogonialstemcellsviathenoncanonicalwntpathwayinmice AT shenminyang strawberrynotch1sbno1promotesproliferationofspermatogonialstemcellsviathenoncanonicalwntpathwayinmice AT hongli strawberrynotch1sbno1promotesproliferationofspermatogonialstemcellsviathenoncanonicalwntpathwayinmice AT bozheng strawberrynotch1sbno1promotesproliferationofspermatogonialstemcellsviathenoncanonicalwntpathwayinmice AT xiaoyanhuang strawberrynotch1sbno1promotesproliferationofspermatogonialstemcellsviathenoncanonicalwntpathwayinmice |