Quorum Sensing Activity of Enterobacter asburiae Isolated from Lettuce Leaves
Bacterial communication or quorum sensing (QS) is achieved via sensing of QS signaling molecules consisting of oligopeptides in Gram-positive bacteria and N-acyl homoserine lactones (AHL) in most Gram-negative bacteria. In this study, Enterobacteriaceae isolates from Batavia lettuce were screened fo...
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| Format: | Article |
| Language: | English |
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MDPI AG
2013-10-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/13/10/14189 |
| _version_ | 1798024270785282048 |
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| author | Kok-Gan Chan Jian Woon Chen Wai-Fong Yin Yin Yin Lau Joanita Sulaiman |
| author_facet | Kok-Gan Chan Jian Woon Chen Wai-Fong Yin Yin Yin Lau Joanita Sulaiman |
| author_sort | Kok-Gan Chan |
| collection | DOAJ |
| description | Bacterial communication or quorum sensing (QS) is achieved via sensing of QS signaling molecules consisting of oligopeptides in Gram-positive bacteria and N-acyl homoserine lactones (AHL) in most Gram-negative bacteria. In this study, Enterobacteriaceae isolates from Batavia lettuce were screened for AHL production. Enterobacter asburiae, identified by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) was found to produce short chain AHLs. High resolution triple quadrupole liquid chromatography mass spectrometry (LC/MS) analysis of the E. asburiae spent supernatant confirmed the production of N-butanoyl homoserine lactone (C4-HSL) and N–hexanoyl homoserine lactone (C6-HSL). To the best of our knowledge, this is the first report of AHL production by E. asburiae. |
| first_indexed | 2024-04-11T18:01:03Z |
| format | Article |
| id | doaj.art-6c37f69aae4d4e548de5c56ef0d4aadc |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T18:01:03Z |
| publishDate | 2013-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-6c37f69aae4d4e548de5c56ef0d4aadc2022-12-22T04:10:32ZengMDPI AGSensors1424-82202013-10-011310141891419910.3390/s131014189Quorum Sensing Activity of Enterobacter asburiae Isolated from Lettuce LeavesKok-Gan ChanJian Woon ChenWai-Fong YinYin Yin LauJoanita SulaimanBacterial communication or quorum sensing (QS) is achieved via sensing of QS signaling molecules consisting of oligopeptides in Gram-positive bacteria and N-acyl homoserine lactones (AHL) in most Gram-negative bacteria. In this study, Enterobacteriaceae isolates from Batavia lettuce were screened for AHL production. Enterobacter asburiae, identified by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) was found to produce short chain AHLs. High resolution triple quadrupole liquid chromatography mass spectrometry (LC/MS) analysis of the E. asburiae spent supernatant confirmed the production of N-butanoyl homoserine lactone (C4-HSL) and N–hexanoyl homoserine lactone (C6-HSL). To the best of our knowledge, this is the first report of AHL production by E. asburiae.http://www.mdpi.com/1424-8220/13/10/14189Enterobacter asburiaeMALDI-TOFmass spectrometryN-acyl homoserine lactoneN–hexanoyl homoserine lactoneN-butanoyl homoserine lactonequorum sensinglettucefood spoilagefood microbiologyfood safety |
| spellingShingle | Kok-Gan Chan Jian Woon Chen Wai-Fong Yin Yin Yin Lau Joanita Sulaiman Quorum Sensing Activity of Enterobacter asburiae Isolated from Lettuce Leaves Sensors Enterobacter asburiae MALDI-TOF mass spectrometry N-acyl homoserine lactone N–hexanoyl homoserine lactone N-butanoyl homoserine lactone quorum sensing lettuce food spoilage food microbiology food safety |
| title | Quorum Sensing Activity of Enterobacter asburiae Isolated from Lettuce Leaves |
| title_full | Quorum Sensing Activity of Enterobacter asburiae Isolated from Lettuce Leaves |
| title_fullStr | Quorum Sensing Activity of Enterobacter asburiae Isolated from Lettuce Leaves |
| title_full_unstemmed | Quorum Sensing Activity of Enterobacter asburiae Isolated from Lettuce Leaves |
| title_short | Quorum Sensing Activity of Enterobacter asburiae Isolated from Lettuce Leaves |
| title_sort | quorum sensing activity of enterobacter asburiae isolated from lettuce leaves |
| topic | Enterobacter asburiae MALDI-TOF mass spectrometry N-acyl homoserine lactone N–hexanoyl homoserine lactone N-butanoyl homoserine lactone quorum sensing lettuce food spoilage food microbiology food safety |
| url | http://www.mdpi.com/1424-8220/13/10/14189 |
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