Inhibitory Effects of Pectic Polysaccharide Isolated from <i>Diospyros kaki</i> Leaves on Tumor Cell Angiogenesis via VEGF and MMP-9 Regulation
Persimmon leaves are an attractive source of phytochemicals with potential health benefits. However, there are only a few reports on the chemical properties and biological activity of the polysaccharide fractions (PLE-I–III) of persimmon leaves. We evaluated the angiogenesis-inhibiting ability of pe...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-12-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/13/1/64 |
| _version_ | 1797543436761432064 |
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| author | Jun Yeon Park Myoung-Sook Shin |
| author_facet | Jun Yeon Park Myoung-Sook Shin |
| author_sort | Jun Yeon Park |
| collection | DOAJ |
| description | Persimmon leaves are an attractive source of phytochemicals with potential health benefits. However, there are only a few reports on the chemical properties and biological activity of the polysaccharide fractions (PLE-I–III) of persimmon leaves. We evaluated the angiogenesis-inhibiting ability of pectic-polysaccharides. The molecular weight of PLEs was determined using a high-performance size-exclusion chromatography system. Tube formation assay of human umbilical vein endothelial cells (HUVECs) was performed using Matrigel-coated 96-well plates. Matrix metalloproteinase (MMP-9), vascular endothelial growth factor (VEGF), PI3K, Akt, and p38 phosphorylation levels were determined using Western blotting; VEGF and MMP-9 transcript levels were measured using SYBR Green qRT-PCR. PLE-I–III significantly inhibited HUVEC tube formation at 12.5 and 25 μg/mL. Among them, PLE-II showed the strongest anti-tube formation activity, and the mRNA/protein expression of angiogenesis-related factors (VEGF/MMP-9) was significantly reduced by PLE-II. PLE-II also suppressed the phosphorylation of PI3K/AKT and p38, JNK, and NF-κB p65 in HUVECs. These results suggest that the polysaccharide PLE-II isolated from persimmon leaves inhibited VEGF and MMP-9 expression in HUVECs via regulation of PI3K/AKT, p38, JNK, and NF-κB p65 signaling pathways. |
| first_indexed | 2024-03-10T13:45:32Z |
| format | Article |
| id | doaj.art-6c38c76af36f4cb4b65f49e257cc8178 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T13:45:32Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-6c38c76af36f4cb4b65f49e257cc81782023-11-21T02:38:22ZengMDPI AGPolymers2073-43602020-12-011316410.3390/polym13010064Inhibitory Effects of Pectic Polysaccharide Isolated from <i>Diospyros kaki</i> Leaves on Tumor Cell Angiogenesis via VEGF and MMP-9 RegulationJun Yeon Park0Myoung-Sook Shin1Department of Food Science and Biotechnology, Kyonggi University, Suwon-si, Gyeonggi-do 16227, KoreaCollege of Korean Medicine, Gachon University, Seongnam-si, Gyeonggi-do 13120, KoreaPersimmon leaves are an attractive source of phytochemicals with potential health benefits. However, there are only a few reports on the chemical properties and biological activity of the polysaccharide fractions (PLE-I–III) of persimmon leaves. We evaluated the angiogenesis-inhibiting ability of pectic-polysaccharides. The molecular weight of PLEs was determined using a high-performance size-exclusion chromatography system. Tube formation assay of human umbilical vein endothelial cells (HUVECs) was performed using Matrigel-coated 96-well plates. Matrix metalloproteinase (MMP-9), vascular endothelial growth factor (VEGF), PI3K, Akt, and p38 phosphorylation levels were determined using Western blotting; VEGF and MMP-9 transcript levels were measured using SYBR Green qRT-PCR. PLE-I–III significantly inhibited HUVEC tube formation at 12.5 and 25 μg/mL. Among them, PLE-II showed the strongest anti-tube formation activity, and the mRNA/protein expression of angiogenesis-related factors (VEGF/MMP-9) was significantly reduced by PLE-II. PLE-II also suppressed the phosphorylation of PI3K/AKT and p38, JNK, and NF-κB p65 in HUVECs. These results suggest that the polysaccharide PLE-II isolated from persimmon leaves inhibited VEGF and MMP-9 expression in HUVECs via regulation of PI3K/AKT, p38, JNK, and NF-κB p65 signaling pathways.https://www.mdpi.com/2073-4360/13/1/64angiogenesispectic polysaccharideMMP-9VEGF |
| spellingShingle | Jun Yeon Park Myoung-Sook Shin Inhibitory Effects of Pectic Polysaccharide Isolated from <i>Diospyros kaki</i> Leaves on Tumor Cell Angiogenesis via VEGF and MMP-9 Regulation Polymers angiogenesis pectic polysaccharide MMP-9 VEGF |
| title | Inhibitory Effects of Pectic Polysaccharide Isolated from <i>Diospyros kaki</i> Leaves on Tumor Cell Angiogenesis via VEGF and MMP-9 Regulation |
| title_full | Inhibitory Effects of Pectic Polysaccharide Isolated from <i>Diospyros kaki</i> Leaves on Tumor Cell Angiogenesis via VEGF and MMP-9 Regulation |
| title_fullStr | Inhibitory Effects of Pectic Polysaccharide Isolated from <i>Diospyros kaki</i> Leaves on Tumor Cell Angiogenesis via VEGF and MMP-9 Regulation |
| title_full_unstemmed | Inhibitory Effects of Pectic Polysaccharide Isolated from <i>Diospyros kaki</i> Leaves on Tumor Cell Angiogenesis via VEGF and MMP-9 Regulation |
| title_short | Inhibitory Effects of Pectic Polysaccharide Isolated from <i>Diospyros kaki</i> Leaves on Tumor Cell Angiogenesis via VEGF and MMP-9 Regulation |
| title_sort | inhibitory effects of pectic polysaccharide isolated from i diospyros kaki i leaves on tumor cell angiogenesis via vegf and mmp 9 regulation |
| topic | angiogenesis pectic polysaccharide MMP-9 VEGF |
| url | https://www.mdpi.com/2073-4360/13/1/64 |
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