The protein interaction map of bacteriophage lambda
<p>Abstract</p> <p>Background</p> <p>Bacteriophage lambda is a model phage for most other dsDNA phages and has been studied for over 60 years. Although it is probably the best-characterized phage there are still about 20 poorly understood open reading frames in its 48-k...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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BMC
2011-09-01
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| Series: | BMC Microbiology |
| Online Access: | http://www.biomedcentral.com/1471-2180/11/213 |
| _version_ | 1831834546279022592 |
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| author | Uetz Peter Casjens Sherwood Rajagopala Seesandra V |
| author_facet | Uetz Peter Casjens Sherwood Rajagopala Seesandra V |
| author_sort | Uetz Peter |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Bacteriophage lambda is a model phage for most other dsDNA phages and has been studied for over 60 years. Although it is probably the best-characterized phage there are still about 20 poorly understood open reading frames in its 48-kb genome. For a complete understanding we need to know all interactions among its proteins. We have manually curated the lambda literature and compiled a total of 33 interactions that have been found among lambda proteins. We set out to find out how many protein-protein interactions remain to be found in this phage.</p> <p>Results</p> <p>In order to map lambda's interactions, we have cloned 68 out of 73 lambda open reading frames (the "ORFeome") into Gateway vectors and systematically tested all proteins for interactions using exhaustive array-based yeast two-hybrid screens. These screens identified 97 interactions. We found 16 out of 30 previously published interactions (53%). We have also found at least 18 new plausible interactions among functionally related proteins. All previously found and new interactions are combined into structural and network models of phage lambda.</p> <p>Conclusions</p> <p>Phage lambda serves as a benchmark for future studies of protein interactions among phage, viruses in general, or large protein assemblies. We conclude that we could not find all the known interactions because they require chaperones, post-translational modifications, or multiple proteins for their interactions. The lambda protein network connects 12 proteins of unknown function with well characterized proteins, which should shed light on the functional associations of these uncharacterized proteins.</p> |
| first_indexed | 2024-12-23T04:16:55Z |
| format | Article |
| id | doaj.art-6c38e0e58bc344559dc0736185e67607 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-23T04:16:55Z |
| publishDate | 2011-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-6c38e0e58bc344559dc0736185e676072022-12-21T18:00:22ZengBMCBMC Microbiology1471-21802011-09-0111121310.1186/1471-2180-11-213The protein interaction map of bacteriophage lambdaUetz PeterCasjens SherwoodRajagopala Seesandra V<p>Abstract</p> <p>Background</p> <p>Bacteriophage lambda is a model phage for most other dsDNA phages and has been studied for over 60 years. Although it is probably the best-characterized phage there are still about 20 poorly understood open reading frames in its 48-kb genome. For a complete understanding we need to know all interactions among its proteins. We have manually curated the lambda literature and compiled a total of 33 interactions that have been found among lambda proteins. We set out to find out how many protein-protein interactions remain to be found in this phage.</p> <p>Results</p> <p>In order to map lambda's interactions, we have cloned 68 out of 73 lambda open reading frames (the "ORFeome") into Gateway vectors and systematically tested all proteins for interactions using exhaustive array-based yeast two-hybrid screens. These screens identified 97 interactions. We found 16 out of 30 previously published interactions (53%). We have also found at least 18 new plausible interactions among functionally related proteins. All previously found and new interactions are combined into structural and network models of phage lambda.</p> <p>Conclusions</p> <p>Phage lambda serves as a benchmark for future studies of protein interactions among phage, viruses in general, or large protein assemblies. We conclude that we could not find all the known interactions because they require chaperones, post-translational modifications, or multiple proteins for their interactions. The lambda protein network connects 12 proteins of unknown function with well characterized proteins, which should shed light on the functional associations of these uncharacterized proteins.</p>http://www.biomedcentral.com/1471-2180/11/213 |
| spellingShingle | Uetz Peter Casjens Sherwood Rajagopala Seesandra V The protein interaction map of bacteriophage lambda BMC Microbiology |
| title | The protein interaction map of bacteriophage lambda |
| title_full | The protein interaction map of bacteriophage lambda |
| title_fullStr | The protein interaction map of bacteriophage lambda |
| title_full_unstemmed | The protein interaction map of bacteriophage lambda |
| title_short | The protein interaction map of bacteriophage lambda |
| title_sort | protein interaction map of bacteriophage lambda |
| url | http://www.biomedcentral.com/1471-2180/11/213 |
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