In vivo studies of sterol and squalene secretion by human skin
This work was aimed at studying the quantity and composition of sterols and squalene secreted by the human skin. Lipids secreted by the entire skin were recovered by Soxhlet extraction of the clothing worn by a patient for 24 hr with a chloroform–methanol azeotrope and by extracting the water of a s...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
1974-11-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520367572 |
| _version_ | 1818385176730796032 |
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| author | T. Nikkari P.H. Schreibman E.H. Ahrens, Jr. |
| author_facet | T. Nikkari P.H. Schreibman E.H. Ahrens, Jr. |
| author_sort | T. Nikkari |
| collection | DOAJ |
| description | This work was aimed at studying the quantity and composition of sterols and squalene secreted by the human skin. Lipids secreted by the entire skin were recovered by Soxhlet extraction of the clothing worn by a patient for 24 hr with a chloroform–methanol azeotrope and by extracting the water of a shower taken by the patient at the end of the 24-hr period. Squalene and sterols were quantified by gas–liquid chromatography. Plant sterols were separated from total sterols by thinlayer chromatography. Free and esterified cholesterol were separated by digitonin precipitation. In eight adults, seven of them with hyperlipoproteinemia, the total skin secretion of cholesterol ranged from 59 to 108 mg/day, with a mean of 88 ± 17 (SD) mg/day. There was no difference in cholesterol secretion between the normocholesterolemic individual and the hypercholesterolemic ones, nor were there any differences according to type of hyperlipoproteinemia. Free cholesterol amounted to 54 ± 5% of the total cholesterol. The secretion of squalene ranged from 125 to 475 mg/day in five patients. The secretion of both squalene and cholesterol was quite constant for any individual on a given diet. Cholesterol constituted 95.6 ± 0.5% of the digitonin-precipitable total body surface sterols of eight patients, and lathosterol, the next largest fraction, 3.4 ± 0.4%. Total plant sterols formed only 0.65 ± 0.38% and β-sitosterol 0.35 ± 0.23% of the skin surface sterols in six patients whose dietary β-sitosterol intake ranged from 230 to 3400 mg/day. |
| first_indexed | 2024-12-14T03:34:00Z |
| format | Article |
| id | doaj.art-6c38f709c32148c9aed92b6c6d7ef56d |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-14T03:34:00Z |
| publishDate | 1974-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-6c38f709c32148c9aed92b6c6d7ef56d2022-12-21T23:18:40ZengElsevierJournal of Lipid Research0022-22751974-11-01156563573In vivo studies of sterol and squalene secretion by human skinT. Nikkari0P.H. Schreibman1E.H. Ahrens, Jr.2The Rockefeller University, New York 10021The Rockefeller University, New York 10021The Rockefeller University, New York 10021This work was aimed at studying the quantity and composition of sterols and squalene secreted by the human skin. Lipids secreted by the entire skin were recovered by Soxhlet extraction of the clothing worn by a patient for 24 hr with a chloroform–methanol azeotrope and by extracting the water of a shower taken by the patient at the end of the 24-hr period. Squalene and sterols were quantified by gas–liquid chromatography. Plant sterols were separated from total sterols by thinlayer chromatography. Free and esterified cholesterol were separated by digitonin precipitation. In eight adults, seven of them with hyperlipoproteinemia, the total skin secretion of cholesterol ranged from 59 to 108 mg/day, with a mean of 88 ± 17 (SD) mg/day. There was no difference in cholesterol secretion between the normocholesterolemic individual and the hypercholesterolemic ones, nor were there any differences according to type of hyperlipoproteinemia. Free cholesterol amounted to 54 ± 5% of the total cholesterol. The secretion of squalene ranged from 125 to 475 mg/day in five patients. The secretion of both squalene and cholesterol was quite constant for any individual on a given diet. Cholesterol constituted 95.6 ± 0.5% of the digitonin-precipitable total body surface sterols of eight patients, and lathosterol, the next largest fraction, 3.4 ± 0.4%. Total plant sterols formed only 0.65 ± 0.38% and β-sitosterol 0.35 ± 0.23% of the skin surface sterols in six patients whose dietary β-sitosterol intake ranged from 230 to 3400 mg/day.http://www.sciencedirect.com/science/article/pii/S0022227520367572skin surface lipidscholesterolplant sterolsβ-sitosterol |
| spellingShingle | T. Nikkari P.H. Schreibman E.H. Ahrens, Jr. In vivo studies of sterol and squalene secretion by human skin Journal of Lipid Research skin surface lipids cholesterol plant sterols β-sitosterol |
| title | In vivo studies of sterol and squalene secretion by human skin |
| title_full | In vivo studies of sterol and squalene secretion by human skin |
| title_fullStr | In vivo studies of sterol and squalene secretion by human skin |
| title_full_unstemmed | In vivo studies of sterol and squalene secretion by human skin |
| title_short | In vivo studies of sterol and squalene secretion by human skin |
| title_sort | in vivo studies of sterol and squalene secretion by human skin |
| topic | skin surface lipids cholesterol plant sterols β-sitosterol |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520367572 |
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