Antioxidant Fucoidans Obtained from Tropical Seaweed Protect Pre-Osteoblastic Cells from Hydrogen Peroxide-Induced Damage
Some antioxidant compounds decrease the amount of intracellular reactive oxygen species (ROS) and consequently reduce the deleterious effects of ROS in osteoblasts. Thus, these compounds fight against osteoporosis. Brown seaweeds are a rich source of antioxidant fucose-containing sulfated polysaccha...
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| Format: | Article |
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MDPI AG
2019-08-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/17/9/506 |
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| author | Gabriel Pereira Fidelis Cynthia Haynara Ferreira Silva Leonardo Thiago Duarte Barreto Nobre Valquíria Pereira Medeiros Hugo Alexandre Oliveira Rocha Leandro Silva Costa |
| author_facet | Gabriel Pereira Fidelis Cynthia Haynara Ferreira Silva Leonardo Thiago Duarte Barreto Nobre Valquíria Pereira Medeiros Hugo Alexandre Oliveira Rocha Leandro Silva Costa |
| author_sort | Gabriel Pereira Fidelis |
| collection | DOAJ |
| description | Some antioxidant compounds decrease the amount of intracellular reactive oxygen species (ROS) and consequently reduce the deleterious effects of ROS in osteoblasts. Thus, these compounds fight against osteoporosis. Brown seaweeds are a rich source of antioxidant fucose-containing sulfated polysaccharides (fucans and fucoidans). We obtained six fucoidans (FRFs)—F0.3, F0.5, F0.7, F1.0, F1.5, and F2.1—from <i>Dictyota mertensii</i> by proteolytic digestion followed by sequential acetone precipitation. Except for F0.3, all FRFs showed antioxidant activity in different in vitro tests. In pre- osteoblast-like cells (MC3T3-L1) exposed to H<sub>2</sub>O<sub>2</sub>-oxidative stress, caspase-3 and caspase-9 were activated, resulting in apoptosis of the cells. We also observed a decrease in superoxide dismutase (SOD) and alkaline phosphatase (ALP) activity. The antioxidant FRFs protected the cells from the oxidative damage caused by H<sub>2</sub>O<sub>2</sub>, decreasing intracellular ROS and caspase activation, and increasing SOD activity. The most effective protection against damage was provided by F0.7, F1.5, and F2.1. At 0.5 mg/mL, these FRFs also suppressed the H<sub>2</sub>O<sub>2</sub>-mediated inhibition of ALP activity. The data indicated that FRFs F0.7, F1.5, and F2.1 from <i>D. mertensii</i> were antioxidants that protected bone tissue from oxidative stress and could represent possible adjuvants for the treatment of bone fragility through counteracting oxidative phenomena. |
| first_indexed | 2024-04-13T08:18:57Z |
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| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-13T08:18:57Z |
| publishDate | 2019-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-6c398554bdc746a4ab66b8a0e0f523cb2022-12-22T02:54:42ZengMDPI AGMarine Drugs1660-33972019-08-0117950610.3390/md17090506md17090506Antioxidant Fucoidans Obtained from Tropical Seaweed Protect Pre-Osteoblastic Cells from Hydrogen Peroxide-Induced DamageGabriel Pereira Fidelis0Cynthia Haynara Ferreira Silva1Leonardo Thiago Duarte Barreto Nobre2Valquíria Pereira Medeiros3Hugo Alexandre Oliveira Rocha4Leandro Silva Costa5Department of Biochemistry, Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte 59.078-970, BrazilDepartment of Biochemistry, Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte 59.078-970, BrazilDepartment of Biochemistry, Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte 59.078-970, BrazilDepartment of Biochemistry, Universidade Federal de Juiz de Fora, Juiz de Fora, Minas Gerais 36036-900, BrazilDepartment of Biochemistry, Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte 59.078-970, BrazilInstituto Federal de Educação, Ciência, e Tecnologia do Rio Grande do Norte (IFRN), Rio Grande do Norte, Canguaretama, Rio Grande do Norte 59.500-000, BrazilSome antioxidant compounds decrease the amount of intracellular reactive oxygen species (ROS) and consequently reduce the deleterious effects of ROS in osteoblasts. Thus, these compounds fight against osteoporosis. Brown seaweeds are a rich source of antioxidant fucose-containing sulfated polysaccharides (fucans and fucoidans). We obtained six fucoidans (FRFs)—F0.3, F0.5, F0.7, F1.0, F1.5, and F2.1—from <i>Dictyota mertensii</i> by proteolytic digestion followed by sequential acetone precipitation. Except for F0.3, all FRFs showed antioxidant activity in different in vitro tests. In pre- osteoblast-like cells (MC3T3-L1) exposed to H<sub>2</sub>O<sub>2</sub>-oxidative stress, caspase-3 and caspase-9 were activated, resulting in apoptosis of the cells. We also observed a decrease in superoxide dismutase (SOD) and alkaline phosphatase (ALP) activity. The antioxidant FRFs protected the cells from the oxidative damage caused by H<sub>2</sub>O<sub>2</sub>, decreasing intracellular ROS and caspase activation, and increasing SOD activity. The most effective protection against damage was provided by F0.7, F1.5, and F2.1. At 0.5 mg/mL, these FRFs also suppressed the H<sub>2</sub>O<sub>2</sub>-mediated inhibition of ALP activity. The data indicated that FRFs F0.7, F1.5, and F2.1 from <i>D. mertensii</i> were antioxidants that protected bone tissue from oxidative stress and could represent possible adjuvants for the treatment of bone fragility through counteracting oxidative phenomena.https://www.mdpi.com/1660-3397/17/9/506bone cellssulfated polysaccharidesosteoporosisbrown seaweedoxidative damage |
| spellingShingle | Gabriel Pereira Fidelis Cynthia Haynara Ferreira Silva Leonardo Thiago Duarte Barreto Nobre Valquíria Pereira Medeiros Hugo Alexandre Oliveira Rocha Leandro Silva Costa Antioxidant Fucoidans Obtained from Tropical Seaweed Protect Pre-Osteoblastic Cells from Hydrogen Peroxide-Induced Damage Marine Drugs bone cells sulfated polysaccharides osteoporosis brown seaweed oxidative damage |
| title | Antioxidant Fucoidans Obtained from Tropical Seaweed Protect Pre-Osteoblastic Cells from Hydrogen Peroxide-Induced Damage |
| title_full | Antioxidant Fucoidans Obtained from Tropical Seaweed Protect Pre-Osteoblastic Cells from Hydrogen Peroxide-Induced Damage |
| title_fullStr | Antioxidant Fucoidans Obtained from Tropical Seaweed Protect Pre-Osteoblastic Cells from Hydrogen Peroxide-Induced Damage |
| title_full_unstemmed | Antioxidant Fucoidans Obtained from Tropical Seaweed Protect Pre-Osteoblastic Cells from Hydrogen Peroxide-Induced Damage |
| title_short | Antioxidant Fucoidans Obtained from Tropical Seaweed Protect Pre-Osteoblastic Cells from Hydrogen Peroxide-Induced Damage |
| title_sort | antioxidant fucoidans obtained from tropical seaweed protect pre osteoblastic cells from hydrogen peroxide induced damage |
| topic | bone cells sulfated polysaccharides osteoporosis brown seaweed oxidative damage |
| url | https://www.mdpi.com/1660-3397/17/9/506 |
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