The Effect of <i>Curcuma phaeocaulis</i> Valeton (Zingiberaceae) Extract on Prion Propagation in Cell-Based and Animal Models
Prion diseases are neurodegenerative disorders in humans and animals for which no therapies are currently available. Here, we report that <i>Curcuma phaeocaulis</i> Valeton (Zingiberaceae) (<i>CpV</i>) extract was partly effective in decreasing prion aggregation and propagati...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-12-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/1/182 |
| _version_ | 1797406842521911296 |
|---|---|
| author | Sungeun Lee Hakmin Lee Jaehyeon Kim Ji Hoon Kim Eun Mei Gao Yoonjeong Lee Miryeong Yoo Trang H. T. Trinh Jieun Kim Chul Young Kim Chongsuk Ryou |
| author_facet | Sungeun Lee Hakmin Lee Jaehyeon Kim Ji Hoon Kim Eun Mei Gao Yoonjeong Lee Miryeong Yoo Trang H. T. Trinh Jieun Kim Chul Young Kim Chongsuk Ryou |
| author_sort | Sungeun Lee |
| collection | DOAJ |
| description | Prion diseases are neurodegenerative disorders in humans and animals for which no therapies are currently available. Here, we report that <i>Curcuma phaeocaulis</i> Valeton (Zingiberaceae) (<i>CpV</i>) extract was partly effective in decreasing prion aggregation and propagation in both in vitro and in vivo models. <i>CpV</i> extract inhibited self-aggregation of recombinant prion protein (PrP) in a test tube assay and decreased the accumulation of scrapie PrP (PrP<sup>Sc</sup>) in ScN2a cells, a cultured neuroblastoma cell line with chronic prion infection, in a concentration-dependent manner. <i>CpV</i> extract also modified the course of the disease in mice inoculated with mouse-adapted scrapie prions, completely preventing the onset of prion disease in three of eight mice. Biochemical and neuropathological analyses revealed a statistically significant reduction in PrP<sup>Sc</sup> accumulation, spongiosis, astrogliosis, and microglia activation in the brains of mice that avoided disease onset. Furthermore, PrP<sup>Sc</sup> accumulation in the spleen of mice was also reduced. <i>CpV</i> extract precluded prion infection in cultured cells as demonstrated by the modified standard scrapie cell assay. This study suggests that <i>CpV</i> extract could contribute to investigating the modulation of prion propagation. |
| first_indexed | 2024-03-09T03:31:30Z |
| format | Article |
| id | doaj.art-6c3c6e84390440029392c152432f4e9e |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T03:31:30Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-6c3c6e84390440029392c152432f4e9e2023-12-03T14:54:07ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-12-0124118210.3390/ijms24010182The Effect of <i>Curcuma phaeocaulis</i> Valeton (Zingiberaceae) Extract on Prion Propagation in Cell-Based and Animal ModelsSungeun Lee0Hakmin Lee1Jaehyeon Kim2Ji Hoon Kim3Eun Mei Gao4Yoonjeong Lee5Miryeong Yoo6Trang H. T. Trinh7Jieun Kim8Chul Young Kim9Chongsuk Ryou10Department of Pharmacy, College of Pharmacy and Institute of Pharmaceutical Science & Technology, Hanyang University, 55 Hanyangdaehak-ro, Sangnok-gu, Ansan 15588, Gyeonggi-do, Republic of KoreaDepartment of Pharmacy, College of Pharmacy and Institute of Pharmaceutical Science & Technology, Hanyang University, 55 Hanyangdaehak-ro, Sangnok-gu, Ansan 15588, Gyeonggi-do, Republic of KoreaDepartment of Pharmacy, College of Pharmacy and Institute of Pharmaceutical Science & Technology, Hanyang University, 55 Hanyangdaehak-ro, Sangnok-gu, Ansan 15588, Gyeonggi-do, Republic of KoreaDepartment of Pharmacy, College of Pharmacy and Institute of Pharmaceutical Science & Technology, Hanyang University, 55 Hanyangdaehak-ro, Sangnok-gu, Ansan 15588, Gyeonggi-do, Republic of KoreaDepartment of Pharmacy, College of Pharmacy and Institute of Pharmaceutical Science & Technology, Hanyang University, 55 Hanyangdaehak-ro, Sangnok-gu, Ansan 15588, Gyeonggi-do, Republic of KoreaDepartment of Pharmacy, College of Pharmacy and Institute of Pharmaceutical Science & Technology, Hanyang University, 55 Hanyangdaehak-ro, Sangnok-gu, Ansan 15588, Gyeonggi-do, Republic of KoreaDepartment of Pharmacy, College of Pharmacy and Institute of Pharmaceutical Science & Technology, Hanyang University, 55 Hanyangdaehak-ro, Sangnok-gu, Ansan 15588, Gyeonggi-do, Republic of KoreaDepartment of Pharmacy, College of Pharmacy and Institute of Pharmaceutical Science & Technology, Hanyang University, 55 Hanyangdaehak-ro, Sangnok-gu, Ansan 15588, Gyeonggi-do, Republic of KoreaDepartment of Pharmacy, College of Pharmacy and Institute of Pharmaceutical Science & Technology, Hanyang University, 55 Hanyangdaehak-ro, Sangnok-gu, Ansan 15588, Gyeonggi-do, Republic of KoreaDepartment of Pharmacy, College of Pharmacy and Institute of Pharmaceutical Science & Technology, Hanyang University, 55 Hanyangdaehak-ro, Sangnok-gu, Ansan 15588, Gyeonggi-do, Republic of KoreaDepartment of Pharmacy, College of Pharmacy and Institute of Pharmaceutical Science & Technology, Hanyang University, 55 Hanyangdaehak-ro, Sangnok-gu, Ansan 15588, Gyeonggi-do, Republic of KoreaPrion diseases are neurodegenerative disorders in humans and animals for which no therapies are currently available. Here, we report that <i>Curcuma phaeocaulis</i> Valeton (Zingiberaceae) (<i>CpV</i>) extract was partly effective in decreasing prion aggregation and propagation in both in vitro and in vivo models. <i>CpV</i> extract inhibited self-aggregation of recombinant prion protein (PrP) in a test tube assay and decreased the accumulation of scrapie PrP (PrP<sup>Sc</sup>) in ScN2a cells, a cultured neuroblastoma cell line with chronic prion infection, in a concentration-dependent manner. <i>CpV</i> extract also modified the course of the disease in mice inoculated with mouse-adapted scrapie prions, completely preventing the onset of prion disease in three of eight mice. Biochemical and neuropathological analyses revealed a statistically significant reduction in PrP<sup>Sc</sup> accumulation, spongiosis, astrogliosis, and microglia activation in the brains of mice that avoided disease onset. Furthermore, PrP<sup>Sc</sup> accumulation in the spleen of mice was also reduced. <i>CpV</i> extract precluded prion infection in cultured cells as demonstrated by the modified standard scrapie cell assay. This study suggests that <i>CpV</i> extract could contribute to investigating the modulation of prion propagation.https://www.mdpi.com/1422-0067/24/1/182prionsinhibition<i>Curcuma phaeocaulis</i> Valetonnatural products |
| spellingShingle | Sungeun Lee Hakmin Lee Jaehyeon Kim Ji Hoon Kim Eun Mei Gao Yoonjeong Lee Miryeong Yoo Trang H. T. Trinh Jieun Kim Chul Young Kim Chongsuk Ryou The Effect of <i>Curcuma phaeocaulis</i> Valeton (Zingiberaceae) Extract on Prion Propagation in Cell-Based and Animal Models International Journal of Molecular Sciences prions inhibition <i>Curcuma phaeocaulis</i> Valeton natural products |
| title | The Effect of <i>Curcuma phaeocaulis</i> Valeton (Zingiberaceae) Extract on Prion Propagation in Cell-Based and Animal Models |
| title_full | The Effect of <i>Curcuma phaeocaulis</i> Valeton (Zingiberaceae) Extract on Prion Propagation in Cell-Based and Animal Models |
| title_fullStr | The Effect of <i>Curcuma phaeocaulis</i> Valeton (Zingiberaceae) Extract on Prion Propagation in Cell-Based and Animal Models |
| title_full_unstemmed | The Effect of <i>Curcuma phaeocaulis</i> Valeton (Zingiberaceae) Extract on Prion Propagation in Cell-Based and Animal Models |
| title_short | The Effect of <i>Curcuma phaeocaulis</i> Valeton (Zingiberaceae) Extract on Prion Propagation in Cell-Based and Animal Models |
| title_sort | effect of i curcuma phaeocaulis i valeton zingiberaceae extract on prion propagation in cell based and animal models |
| topic | prions inhibition <i>Curcuma phaeocaulis</i> Valeton natural products |
| url | https://www.mdpi.com/1422-0067/24/1/182 |
| work_keys_str_mv | AT sungeunlee theeffectoficurcumaphaeocaulisivaletonzingiberaceaeextractonprionpropagationincellbasedandanimalmodels AT hakminlee theeffectoficurcumaphaeocaulisivaletonzingiberaceaeextractonprionpropagationincellbasedandanimalmodels AT jaehyeonkim theeffectoficurcumaphaeocaulisivaletonzingiberaceaeextractonprionpropagationincellbasedandanimalmodels AT jihoonkim theeffectoficurcumaphaeocaulisivaletonzingiberaceaeextractonprionpropagationincellbasedandanimalmodels AT eunmeigao theeffectoficurcumaphaeocaulisivaletonzingiberaceaeextractonprionpropagationincellbasedandanimalmodels AT yoonjeonglee theeffectoficurcumaphaeocaulisivaletonzingiberaceaeextractonprionpropagationincellbasedandanimalmodels AT miryeongyoo theeffectoficurcumaphaeocaulisivaletonzingiberaceaeextractonprionpropagationincellbasedandanimalmodels AT tranghttrinh theeffectoficurcumaphaeocaulisivaletonzingiberaceaeextractonprionpropagationincellbasedandanimalmodels AT jieunkim theeffectoficurcumaphaeocaulisivaletonzingiberaceaeextractonprionpropagationincellbasedandanimalmodels AT chulyoungkim theeffectoficurcumaphaeocaulisivaletonzingiberaceaeextractonprionpropagationincellbasedandanimalmodels AT chongsukryou theeffectoficurcumaphaeocaulisivaletonzingiberaceaeextractonprionpropagationincellbasedandanimalmodels AT sungeunlee effectoficurcumaphaeocaulisivaletonzingiberaceaeextractonprionpropagationincellbasedandanimalmodels AT hakminlee effectoficurcumaphaeocaulisivaletonzingiberaceaeextractonprionpropagationincellbasedandanimalmodels AT jaehyeonkim effectoficurcumaphaeocaulisivaletonzingiberaceaeextractonprionpropagationincellbasedandanimalmodels AT jihoonkim effectoficurcumaphaeocaulisivaletonzingiberaceaeextractonprionpropagationincellbasedandanimalmodels AT eunmeigao effectoficurcumaphaeocaulisivaletonzingiberaceaeextractonprionpropagationincellbasedandanimalmodels AT yoonjeonglee effectoficurcumaphaeocaulisivaletonzingiberaceaeextractonprionpropagationincellbasedandanimalmodels AT miryeongyoo effectoficurcumaphaeocaulisivaletonzingiberaceaeextractonprionpropagationincellbasedandanimalmodels AT tranghttrinh effectoficurcumaphaeocaulisivaletonzingiberaceaeextractonprionpropagationincellbasedandanimalmodels AT jieunkim effectoficurcumaphaeocaulisivaletonzingiberaceaeextractonprionpropagationincellbasedandanimalmodels AT chulyoungkim effectoficurcumaphaeocaulisivaletonzingiberaceaeextractonprionpropagationincellbasedandanimalmodels AT chongsukryou effectoficurcumaphaeocaulisivaletonzingiberaceaeextractonprionpropagationincellbasedandanimalmodels |