TALEN outperforms Cas9 in editing heterochromatin target sites
While Cas9 outperforms TALENs in euchromatin, it is less efficient in heterochromatic regions. Here the authors, using single-molecule imaging, show that Cas9 uses a less efficient search strategy compared to TALENs in these regions.
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-01-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-020-20672-5 |
| _version_ | 1818420550539673600 |
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| author | Surbhi Jain Saurabh Shukla Che Yang Meng Zhang Zia Fatma Manasi Lingamaneni Shireen Abesteh Stephan Thomas Lane Xiong Xiong Yuchuan Wang Charles M. Schroeder Paul R. Selvin Huimin Zhao |
| author_facet | Surbhi Jain Saurabh Shukla Che Yang Meng Zhang Zia Fatma Manasi Lingamaneni Shireen Abesteh Stephan Thomas Lane Xiong Xiong Yuchuan Wang Charles M. Schroeder Paul R. Selvin Huimin Zhao |
| author_sort | Surbhi Jain |
| collection | DOAJ |
| description | While Cas9 outperforms TALENs in euchromatin, it is less efficient in heterochromatic regions. Here the authors, using single-molecule imaging, show that Cas9 uses a less efficient search strategy compared to TALENs in these regions. |
| first_indexed | 2024-12-14T12:56:15Z |
| format | Article |
| id | doaj.art-6c3cb81693a24a32a018bf1763f7bcf5 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-14T12:56:15Z |
| publishDate | 2021-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-6c3cb81693a24a32a018bf1763f7bcf52022-12-21T23:00:33ZengNature PortfolioNature Communications2041-17232021-01-0112111010.1038/s41467-020-20672-5TALEN outperforms Cas9 in editing heterochromatin target sitesSurbhi Jain0Saurabh Shukla1Che Yang2Meng Zhang3Zia Fatma4Manasi Lingamaneni5Shireen Abesteh6Stephan Thomas Lane7Xiong Xiong8Yuchuan Wang9Charles M. Schroeder10Paul R. Selvin11Huimin Zhao12Department of Biochemistry, School of Molecular and Cellular Biology, University of Illinois at Urbana−ChampaignDepartment of Chemical and Biomolecular Engineering, University of Illinois at Urbana−ChampaignDepartment of Biochemistry, School of Molecular and Cellular Biology, University of Illinois at Urbana−ChampaignDepartment of Chemical and Biomolecular Engineering, University of Illinois at Urbana−ChampaignDepartment of Chemical and Biomolecular Engineering, University of Illinois at Urbana−ChampaignDepartment of Biochemistry, School of Molecular and Cellular Biology, University of Illinois at Urbana−ChampaignDepartment of Biochemistry, School of Molecular and Cellular Biology, University of Illinois at Urbana−ChampaignCarl R. Woese Institute for Genomic Biology, University of Illinois at Urbana−ChampaignDepartment of Chemical and Biomolecular Engineering, University of Illinois at Urbana−ChampaignComputational Biology Department, School of Computer Science, Carnegie Mellon UniversityDepartment of Chemical and Biomolecular Engineering, University of Illinois at Urbana−ChampaignCenter for the Physics of Living Cells, University of Illinois at Urbana−ChampaignDepartment of Biochemistry, School of Molecular and Cellular Biology, University of Illinois at Urbana−ChampaignWhile Cas9 outperforms TALENs in euchromatin, it is less efficient in heterochromatic regions. Here the authors, using single-molecule imaging, show that Cas9 uses a less efficient search strategy compared to TALENs in these regions.https://doi.org/10.1038/s41467-020-20672-5 |
| spellingShingle | Surbhi Jain Saurabh Shukla Che Yang Meng Zhang Zia Fatma Manasi Lingamaneni Shireen Abesteh Stephan Thomas Lane Xiong Xiong Yuchuan Wang Charles M. Schroeder Paul R. Selvin Huimin Zhao TALEN outperforms Cas9 in editing heterochromatin target sites Nature Communications |
| title | TALEN outperforms Cas9 in editing heterochromatin target sites |
| title_full | TALEN outperforms Cas9 in editing heterochromatin target sites |
| title_fullStr | TALEN outperforms Cas9 in editing heterochromatin target sites |
| title_full_unstemmed | TALEN outperforms Cas9 in editing heterochromatin target sites |
| title_short | TALEN outperforms Cas9 in editing heterochromatin target sites |
| title_sort | talen outperforms cas9 in editing heterochromatin target sites |
| url | https://doi.org/10.1038/s41467-020-20672-5 |
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