Suppressive Effects of Bee Venom-Derived Phospholipase A2 on Mechanical Allodynia in a Rat Model of Neuropathic Pain
Bee venom (BV) has a long history of being used in traditional Korean medicine to relieve pain. Here, we investigated the effect of BV-derived phospholipase A2 (bvPLA2), a major component of BV, on peripheral nerve injury-induced neuropathic pain in rats. Spinal nerve ligation (SNL) was performed in...
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MDPI AG
2019-08-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/11/8/477 |
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| author | Seunghui Woo Geehoon Chung Hyunsu Bae Sun Kwang Kim |
| author_facet | Seunghui Woo Geehoon Chung Hyunsu Bae Sun Kwang Kim |
| author_sort | Seunghui Woo |
| collection | DOAJ |
| description | Bee venom (BV) has a long history of being used in traditional Korean medicine to relieve pain. Here, we investigated the effect of BV-derived phospholipase A2 (bvPLA2), a major component of BV, on peripheral nerve injury-induced neuropathic pain in rats. Spinal nerve ligation (SNL) was performed in Sprague Dawley rats to induce neuropathic pain, and paw withdrawal thresholds were measured using von Frey test. Mechanical allodynia, the representative symptom of neuropathic pain, was manifested following SNL and persisted for several weeks. The repetitive bvPLA2 treatment (0.2 mg/kg/day, i.p.) for two days significantly relieved the SNL-induced mechanical allodynia. The antiallodynic effect of bvPLA2 was blocked by spinal pretreatment with α1-adrenergic antagonist prazosin (30 μg, i.t.) but not with α2-adrenergic antagonist idazoxan (50 μg, i.t.). Also, the spinal application of α1-adrenergic agonist phenylephrine (50 μg, i.t.) reduced mechanical allodynia. These results indicate that bvPLA2 could relieve nerve injury-induced neuropathic mechanical allodynia through the activation of spinal α1-adrenergic receptors. |
| first_indexed | 2024-04-11T22:23:45Z |
| format | Article |
| id | doaj.art-6c3ed004166e4829a9582950ad2b4f32 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-11T22:23:45Z |
| publishDate | 2019-08-01 |
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| series | Toxins |
| spelling | doaj.art-6c3ed004166e4829a9582950ad2b4f322022-12-22T03:59:56ZengMDPI AGToxins2072-66512019-08-0111847710.3390/toxins11080477toxins11080477Suppressive Effects of Bee Venom-Derived Phospholipase A2 on Mechanical Allodynia in a Rat Model of Neuropathic PainSeunghui Woo0Geehoon Chung1Hyunsu Bae2Sun Kwang Kim3Department of Science in Korean Medicine, Graduate School, Kyung Hee University, Seoul 02447, KoreaDepartment of Physiology, College of Korean Medicine, Kyung Hee University, Seoul 02447, KoreaDepartment of Science in Korean Medicine, Graduate School, Kyung Hee University, Seoul 02447, KoreaDepartment of Science in Korean Medicine, Graduate School, Kyung Hee University, Seoul 02447, KoreaBee venom (BV) has a long history of being used in traditional Korean medicine to relieve pain. Here, we investigated the effect of BV-derived phospholipase A2 (bvPLA2), a major component of BV, on peripheral nerve injury-induced neuropathic pain in rats. Spinal nerve ligation (SNL) was performed in Sprague Dawley rats to induce neuropathic pain, and paw withdrawal thresholds were measured using von Frey test. Mechanical allodynia, the representative symptom of neuropathic pain, was manifested following SNL and persisted for several weeks. The repetitive bvPLA2 treatment (0.2 mg/kg/day, i.p.) for two days significantly relieved the SNL-induced mechanical allodynia. The antiallodynic effect of bvPLA2 was blocked by spinal pretreatment with α1-adrenergic antagonist prazosin (30 μg, i.t.) but not with α2-adrenergic antagonist idazoxan (50 μg, i.t.). Also, the spinal application of α1-adrenergic agonist phenylephrine (50 μg, i.t.) reduced mechanical allodynia. These results indicate that bvPLA2 could relieve nerve injury-induced neuropathic mechanical allodynia through the activation of spinal α1-adrenergic receptors.https://www.mdpi.com/2072-6651/11/8/477phospholipase A2bee venomperipheral nerve injuryneuropathic painmechanical allodyniaspinal cordadrenergic receptor |
| spellingShingle | Seunghui Woo Geehoon Chung Hyunsu Bae Sun Kwang Kim Suppressive Effects of Bee Venom-Derived Phospholipase A2 on Mechanical Allodynia in a Rat Model of Neuropathic Pain Toxins phospholipase A2 bee venom peripheral nerve injury neuropathic pain mechanical allodynia spinal cord adrenergic receptor |
| title | Suppressive Effects of Bee Venom-Derived Phospholipase A2 on Mechanical Allodynia in a Rat Model of Neuropathic Pain |
| title_full | Suppressive Effects of Bee Venom-Derived Phospholipase A2 on Mechanical Allodynia in a Rat Model of Neuropathic Pain |
| title_fullStr | Suppressive Effects of Bee Venom-Derived Phospholipase A2 on Mechanical Allodynia in a Rat Model of Neuropathic Pain |
| title_full_unstemmed | Suppressive Effects of Bee Venom-Derived Phospholipase A2 on Mechanical Allodynia in a Rat Model of Neuropathic Pain |
| title_short | Suppressive Effects of Bee Venom-Derived Phospholipase A2 on Mechanical Allodynia in a Rat Model of Neuropathic Pain |
| title_sort | suppressive effects of bee venom derived phospholipase a2 on mechanical allodynia in a rat model of neuropathic pain |
| topic | phospholipase A2 bee venom peripheral nerve injury neuropathic pain mechanical allodynia spinal cord adrenergic receptor |
| url | https://www.mdpi.com/2072-6651/11/8/477 |
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