Umbilical cord mesenchymal stem cells relieve osteoarthritis in rats through immunoregulation and inhibition of chondrocyte apoptosis
Abstract This study aims to investigate the effectiveness of umbilical cord mesenchymal stem cells (UCMSCs) in treating osteoarthritis (OA). Sprague–Dawley rats were used in in vivo experiments and divided into four groups: normal, OA model, saline, and UCMSC-treated groups (n = 6). An OA model was...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-09-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-42349-x |
| _version_ | 1797559620127948800 |
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| author | Xin Pan Xiongfeng Li Ling Zhang Feifei Wu Qiang Zhang Shasha Xu Chengchun Shen Jinfeng Liang Ruolang Pan |
| author_facet | Xin Pan Xiongfeng Li Ling Zhang Feifei Wu Qiang Zhang Shasha Xu Chengchun Shen Jinfeng Liang Ruolang Pan |
| author_sort | Xin Pan |
| collection | DOAJ |
| description | Abstract This study aims to investigate the effectiveness of umbilical cord mesenchymal stem cells (UCMSCs) in treating osteoarthritis (OA). Sprague–Dawley rats were used in in vivo experiments and divided into four groups: normal, OA model, saline, and UCMSC-treated groups (n = 6). An OA model was established by injecting iodoacetic acid into the joint cavity. The results indicate that UCMSC transplantation significantly reduced joint surface and articular cartilage damage, and the levels of IL-1β, TNF-α, and MMP13 in the joint fluid were significantly reduced after UCMSC treatment. In vitro experiments showed that co-culturing UCMSCs and chondrocytes promoted the expression of aggrecan, COL2, SOX9, and BCL-2; downregulated the expression of BAX and BAD in chondrocytes; and promoted the expression of IL-10 and TGF-β1 in UCMSCs. Additionally, the supernatant of UCMSCs inhibited the expression of IL-1β and TNF-α in the articular cavity and promoted the expression of COL2 and aggrecan in vivo. These effects were impaired when IL-10 and TGF-β1 were removed. Collectively, UCMSC transplantation appears to improve joint pathology, reduce inflammatory factors, and decrease chondrocyte apoptosis, likely through the involvement of IL-10 and TGF-β1, thus providing a potential therapeutic option for patients with OA. |
| first_indexed | 2024-03-10T17:47:53Z |
| format | Article |
| id | doaj.art-6c40a7790a4a4181b9f385f4e0d49d12 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-10T17:47:53Z |
| publishDate | 2023-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-6c40a7790a4a4181b9f385f4e0d49d122023-11-20T09:26:56ZengNature PortfolioScientific Reports2045-23222023-09-011311910.1038/s41598-023-42349-xUmbilical cord mesenchymal stem cells relieve osteoarthritis in rats through immunoregulation and inhibition of chondrocyte apoptosisXin Pan0Xiongfeng Li1Ling Zhang2Feifei Wu3Qiang Zhang4Shasha Xu5Chengchun Shen6Jinfeng Liang7Ruolang Pan8Institute for Cell-Based Drug Development of Zhejiang Province, S-Evans BiosciencesHuzhou Basic and Clinical Translation of Orthopaedics Key Laboratory, Affiliated Huzhou HospitalInstitute for Cell-Based Drug Development of Zhejiang Province, S-Evans BiosciencesInstitute for Cell-Based Drug Development of Zhejiang Province, S-Evans BiosciencesInstitute for Cell-Based Drug Development of Zhejiang Province, S-Evans BiosciencesInstitute for Cell-Based Drug Development of Zhejiang Province, S-Evans BiosciencesHuzhou Basic and Clinical Translation of Orthopaedics Key Laboratory, Affiliated Huzhou HospitalZhejiang Center for Drug & Cosmetic EvaluationInstitute for Cell-Based Drug Development of Zhejiang Province, S-Evans BiosciencesAbstract This study aims to investigate the effectiveness of umbilical cord mesenchymal stem cells (UCMSCs) in treating osteoarthritis (OA). Sprague–Dawley rats were used in in vivo experiments and divided into four groups: normal, OA model, saline, and UCMSC-treated groups (n = 6). An OA model was established by injecting iodoacetic acid into the joint cavity. The results indicate that UCMSC transplantation significantly reduced joint surface and articular cartilage damage, and the levels of IL-1β, TNF-α, and MMP13 in the joint fluid were significantly reduced after UCMSC treatment. In vitro experiments showed that co-culturing UCMSCs and chondrocytes promoted the expression of aggrecan, COL2, SOX9, and BCL-2; downregulated the expression of BAX and BAD in chondrocytes; and promoted the expression of IL-10 and TGF-β1 in UCMSCs. Additionally, the supernatant of UCMSCs inhibited the expression of IL-1β and TNF-α in the articular cavity and promoted the expression of COL2 and aggrecan in vivo. These effects were impaired when IL-10 and TGF-β1 were removed. Collectively, UCMSC transplantation appears to improve joint pathology, reduce inflammatory factors, and decrease chondrocyte apoptosis, likely through the involvement of IL-10 and TGF-β1, thus providing a potential therapeutic option for patients with OA.https://doi.org/10.1038/s41598-023-42349-x |
| spellingShingle | Xin Pan Xiongfeng Li Ling Zhang Feifei Wu Qiang Zhang Shasha Xu Chengchun Shen Jinfeng Liang Ruolang Pan Umbilical cord mesenchymal stem cells relieve osteoarthritis in rats through immunoregulation and inhibition of chondrocyte apoptosis Scientific Reports |
| title | Umbilical cord mesenchymal stem cells relieve osteoarthritis in rats through immunoregulation and inhibition of chondrocyte apoptosis |
| title_full | Umbilical cord mesenchymal stem cells relieve osteoarthritis in rats through immunoregulation and inhibition of chondrocyte apoptosis |
| title_fullStr | Umbilical cord mesenchymal stem cells relieve osteoarthritis in rats through immunoregulation and inhibition of chondrocyte apoptosis |
| title_full_unstemmed | Umbilical cord mesenchymal stem cells relieve osteoarthritis in rats through immunoregulation and inhibition of chondrocyte apoptosis |
| title_short | Umbilical cord mesenchymal stem cells relieve osteoarthritis in rats through immunoregulation and inhibition of chondrocyte apoptosis |
| title_sort | umbilical cord mesenchymal stem cells relieve osteoarthritis in rats through immunoregulation and inhibition of chondrocyte apoptosis |
| url | https://doi.org/10.1038/s41598-023-42349-x |
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