Transcriptomic Evidence of Molecular Mechanisms Underlying the Response of <i>Lactobacillus plantarum</i> WCFS1 to Hydroxytyrosol
This study was aimed to gain new insights into the molecular mechanisms used by <i>Lactobacillus plantarum</i> WCFS1 to respond to hydroxytyrosol (HXT), one of the main and health-relevant plant phenolics present in olive oil. To this goal, whole genome transcriptomic profiling was used...
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| Format: | Article |
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MDPI AG
2020-05-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/9/5/442 |
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| author | Inés Reverón Laura Plaza-Vinuesa Laura Santamaría Juan Carlos Oliveros Blanca de las Rivas Rosario Muñoz Félix López de Felipe |
| author_facet | Inés Reverón Laura Plaza-Vinuesa Laura Santamaría Juan Carlos Oliveros Blanca de las Rivas Rosario Muñoz Félix López de Felipe |
| author_sort | Inés Reverón |
| collection | DOAJ |
| description | This study was aimed to gain new insights into the molecular mechanisms used by <i>Lactobacillus plantarum</i> WCFS1 to respond to hydroxytyrosol (HXT), one of the main and health-relevant plant phenolics present in olive oil. To this goal, whole genome transcriptomic profiling was used to better understand the contribution of differential gene expression in the adaptation to HXT by this microorganism. The transcriptomic profile reveals an HXT-triggered antioxidant response involving genes from the ROS (reactive oxygen species) resistome of <i>L. plantarum</i>, genes coding for H<sub>2</sub>S-producing enzymes and genes involved in the response to thiol-specific oxidative stress. The expression of a set of genes involved in cell wall biogenesis was also upregulated, indicating that this subcellular compartment was a target of HXT. The expression of several MFS (major facilitator superfamily) efflux systems and ABC-transporters was differentially affected by HXT, probably to control its transport across the membrane. <i>L. plantarum</i> transcriptionally reprogrammed nitrogen metabolism and involved the stringent response (SR) to adapt to HXT, as indicated by the reduced expression of genes involved in cell proliferation or related to the metabolism of (p)ppGpp, the molecule that triggers the SR. Our data have identified, at genome scale, the antimicrobial mechanisms of HXT action as well as molecular mechanisms that potentially enable <i>L. plantarum</i> to cope with the effects of this phenolic compound. |
| first_indexed | 2024-03-10T19:41:46Z |
| format | Article |
| id | doaj.art-6c43534407354c238aefe12469b603c2 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T19:41:46Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-6c43534407354c238aefe12469b603c22023-11-20T01:08:25ZengMDPI AGAntioxidants2076-39212020-05-019544210.3390/antiox9050442Transcriptomic Evidence of Molecular Mechanisms Underlying the Response of <i>Lactobacillus plantarum</i> WCFS1 to HydroxytyrosolInés Reverón0Laura Plaza-Vinuesa1Laura Santamaría2Juan Carlos Oliveros3Blanca de las Rivas4Rosario Muñoz5Félix López de Felipe6Laboratorio de Biotecnología Bacteriana. Instituto de Ciencia y Tecnología de los Alimentos y Nutrición (ICTAN-CSIC), 28040 Madrid, SpainLaboratorio de Biotecnología Bacteriana. Instituto de Ciencia y Tecnología de los Alimentos y Nutrición (ICTAN-CSIC), 28040 Madrid, SpainLaboratorio de Biotecnología Bacteriana. Instituto de Ciencia y Tecnología de los Alimentos y Nutrición (ICTAN-CSIC), 28040 Madrid, SpainNational Center for Biotechnology (CNB-CSIC), 28049 Madrid, SpainLaboratorio de Biotecnología Bacteriana. Instituto de Ciencia y Tecnología de los Alimentos y Nutrición (ICTAN-CSIC), 28040 Madrid, SpainLaboratorio de Biotecnología Bacteriana. Instituto de Ciencia y Tecnología de los Alimentos y Nutrición (ICTAN-CSIC), 28040 Madrid, SpainLaboratorio de Biotecnología Bacteriana. Instituto de Ciencia y Tecnología de los Alimentos y Nutrición (ICTAN-CSIC), 28040 Madrid, SpainThis study was aimed to gain new insights into the molecular mechanisms used by <i>Lactobacillus plantarum</i> WCFS1 to respond to hydroxytyrosol (HXT), one of the main and health-relevant plant phenolics present in olive oil. To this goal, whole genome transcriptomic profiling was used to better understand the contribution of differential gene expression in the adaptation to HXT by this microorganism. The transcriptomic profile reveals an HXT-triggered antioxidant response involving genes from the ROS (reactive oxygen species) resistome of <i>L. plantarum</i>, genes coding for H<sub>2</sub>S-producing enzymes and genes involved in the response to thiol-specific oxidative stress. The expression of a set of genes involved in cell wall biogenesis was also upregulated, indicating that this subcellular compartment was a target of HXT. The expression of several MFS (major facilitator superfamily) efflux systems and ABC-transporters was differentially affected by HXT, probably to control its transport across the membrane. <i>L. plantarum</i> transcriptionally reprogrammed nitrogen metabolism and involved the stringent response (SR) to adapt to HXT, as indicated by the reduced expression of genes involved in cell proliferation or related to the metabolism of (p)ppGpp, the molecule that triggers the SR. Our data have identified, at genome scale, the antimicrobial mechanisms of HXT action as well as molecular mechanisms that potentially enable <i>L. plantarum</i> to cope with the effects of this phenolic compound.https://www.mdpi.com/2076-3921/9/5/442hydroxytyrosol<i>Lactobacillus plantarum</i>transcriptomicsantioxidant response |
| spellingShingle | Inés Reverón Laura Plaza-Vinuesa Laura Santamaría Juan Carlos Oliveros Blanca de las Rivas Rosario Muñoz Félix López de Felipe Transcriptomic Evidence of Molecular Mechanisms Underlying the Response of <i>Lactobacillus plantarum</i> WCFS1 to Hydroxytyrosol Antioxidants hydroxytyrosol <i>Lactobacillus plantarum</i> transcriptomics antioxidant response |
| title | Transcriptomic Evidence of Molecular Mechanisms Underlying the Response of <i>Lactobacillus plantarum</i> WCFS1 to Hydroxytyrosol |
| title_full | Transcriptomic Evidence of Molecular Mechanisms Underlying the Response of <i>Lactobacillus plantarum</i> WCFS1 to Hydroxytyrosol |
| title_fullStr | Transcriptomic Evidence of Molecular Mechanisms Underlying the Response of <i>Lactobacillus plantarum</i> WCFS1 to Hydroxytyrosol |
| title_full_unstemmed | Transcriptomic Evidence of Molecular Mechanisms Underlying the Response of <i>Lactobacillus plantarum</i> WCFS1 to Hydroxytyrosol |
| title_short | Transcriptomic Evidence of Molecular Mechanisms Underlying the Response of <i>Lactobacillus plantarum</i> WCFS1 to Hydroxytyrosol |
| title_sort | transcriptomic evidence of molecular mechanisms underlying the response of i lactobacillus plantarum i wcfs1 to hydroxytyrosol |
| topic | hydroxytyrosol <i>Lactobacillus plantarum</i> transcriptomics antioxidant response |
| url | https://www.mdpi.com/2076-3921/9/5/442 |
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