The Detection of SARS-CoV-2 in the Environment: Lessons from Wastewater
Wastewater has historically been an important source of enteric pathogens, as well as a source of unconventational or unexpected pathogens, including those present in the respiratory tract, saliva, urine, and blood. This is the case with SARS-CoV-2, the causative agent of the most recent pandemic. S...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-02-01
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| Series: | Water |
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| Online Access: | https://www.mdpi.com/2073-4441/14/4/599 |
| _version_ | 1797475919931113472 |
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| author | Tasha Marie Santiago-Rodriguez |
| author_facet | Tasha Marie Santiago-Rodriguez |
| author_sort | Tasha Marie Santiago-Rodriguez |
| collection | DOAJ |
| description | Wastewater has historically been an important source of enteric pathogens, as well as a source of unconventational or unexpected pathogens, including those present in the respiratory tract, saliva, urine, and blood. This is the case with SARS-CoV-2, the causative agent of the most recent pandemic. SARS-CoV-2 has been identified in wastewater across various geographical regions prior to, and during, the report of cases. The detection of SARS-CoV-2 in wastewater is usually performed using molecular techniques targeting specific genomic regions. High-throughput sequencing techniques, both untargeted and targeted or amplicon-based, are also being applied in combination with molecular techniques for the detection of SARS-CoV-2 variants to determine the genetic diversity and phylogenetic relatedness. The identification of SARS-CoV-2 in wastewater has a number of epidemiological, biological, and ecological applications, which can be incorporated into future outbreaks, epidemics, or pandemics. |
| first_indexed | 2024-03-09T20:50:39Z |
| format | Article |
| id | doaj.art-6c46c982924c454ba9457de7d10f612d |
| institution | Directory Open Access Journal |
| issn | 2073-4441 |
| language | English |
| last_indexed | 2024-03-09T20:50:39Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Water |
| spelling | doaj.art-6c46c982924c454ba9457de7d10f612d2023-11-23T22:34:29ZengMDPI AGWater2073-44412022-02-0114459910.3390/w14040599The Detection of SARS-CoV-2 in the Environment: Lessons from WastewaterTasha Marie Santiago-Rodriguez0Diversigen, Inc., Houston, TX 77046, USAWastewater has historically been an important source of enteric pathogens, as well as a source of unconventational or unexpected pathogens, including those present in the respiratory tract, saliva, urine, and blood. This is the case with SARS-CoV-2, the causative agent of the most recent pandemic. SARS-CoV-2 has been identified in wastewater across various geographical regions prior to, and during, the report of cases. The detection of SARS-CoV-2 in wastewater is usually performed using molecular techniques targeting specific genomic regions. High-throughput sequencing techniques, both untargeted and targeted or amplicon-based, are also being applied in combination with molecular techniques for the detection of SARS-CoV-2 variants to determine the genetic diversity and phylogenetic relatedness. The identification of SARS-CoV-2 in wastewater has a number of epidemiological, biological, and ecological applications, which can be incorporated into future outbreaks, epidemics, or pandemics.https://www.mdpi.com/2073-4441/14/4/599amplicon sequencinghigh-throughput sequencingSARS-CoV-2 |
| spellingShingle | Tasha Marie Santiago-Rodriguez The Detection of SARS-CoV-2 in the Environment: Lessons from Wastewater Water amplicon sequencing high-throughput sequencing SARS-CoV-2 |
| title | The Detection of SARS-CoV-2 in the Environment: Lessons from Wastewater |
| title_full | The Detection of SARS-CoV-2 in the Environment: Lessons from Wastewater |
| title_fullStr | The Detection of SARS-CoV-2 in the Environment: Lessons from Wastewater |
| title_full_unstemmed | The Detection of SARS-CoV-2 in the Environment: Lessons from Wastewater |
| title_short | The Detection of SARS-CoV-2 in the Environment: Lessons from Wastewater |
| title_sort | detection of sars cov 2 in the environment lessons from wastewater |
| topic | amplicon sequencing high-throughput sequencing SARS-CoV-2 |
| url | https://www.mdpi.com/2073-4441/14/4/599 |
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