Detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) including Variant Analysis by Mass Spectrometry in Placental Tissue
Among neonates, tested positive for SARS-CoV-2, the majority of infections occur through postpartum transmission. Only few reports describe intrauterine or intrapartum SARS-CoV-2 infections in newborns. To understand the route of transmission, detection of the virus or virus nucleic acid in the plac...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/3/604 |
| _version_ | 1827624732870049792 |
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| author | Marina Wierz Beate Sauerbrei Petra Wandernoth Mark Kriegsmann Rita Casadonte Katharina Kriegsmann Jörg Kriegsmann |
| author_facet | Marina Wierz Beate Sauerbrei Petra Wandernoth Mark Kriegsmann Rita Casadonte Katharina Kriegsmann Jörg Kriegsmann |
| author_sort | Marina Wierz |
| collection | DOAJ |
| description | Among neonates, tested positive for SARS-CoV-2, the majority of infections occur through postpartum transmission. Only few reports describe intrauterine or intrapartum SARS-CoV-2 infections in newborns. To understand the route of transmission, detection of the virus or virus nucleic acid in the placenta and amniotic tissue are of special interest. Current methods to detect SARS-CoV-2 in placental tissue are immunohistochemistry, electron microscopy, in-situ hybridization, polymerase chain reaction (PCR) and next-generation sequencing. Recently, we described an alternative method for the detection of viral ribonucleic acid (RNA), by combination of reverse transcriptase-PCR and mass spectrometry (MS) in oropharyngeal and oral swabs. In this report, we could detect SARS-CoV-2 in formal-fixed and paraffin-embedded (FFPE) placental and amniotic tissue by multiplex RT-PCR MS. Additionally, we could identify the British variant (B.1.1.7) of the virus in this tissue by the same methodology. Combination of RT-PCR with MS is a fast and easy method to detect SARS-CoV-2 viral RNA, including specific variants in FFPE tissue. |
| first_indexed | 2024-03-09T12:15:50Z |
| format | Article |
| id | doaj.art-6c49a23994db4e7fa9c54a0aaf2ac110 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T12:15:50Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-6c49a23994db4e7fa9c54a0aaf2ac1102023-11-30T22:46:58ZengMDPI AGViruses1999-49152022-03-0114360410.3390/v14030604Detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) including Variant Analysis by Mass Spectrometry in Placental TissueMarina Wierz0Beate Sauerbrei1Petra Wandernoth2Mark Kriegsmann3Rita Casadonte4Katharina Kriegsmann5Jörg Kriegsmann6MVZ für Histologie, Zytologie und Molekulare Diagnostik Trier, 54296 Trier, GermanyMVZ für Histologie, Zytologie und Molekulare Diagnostik Trier, 54296 Trier, GermanyMVZ für Histologie, Zytologie und Molekulare Diagnostik Trier, 54296 Trier, GermanyInstitute of Pathology, University Hospital Heidelberg, 69120 Heidelberg, GermanyProteopath GmbH, 54926 Trier, GermanyDepartment of Hematology, Oncology and Rheumatology, University Hospital Heidelberg, 69120 Heidelberg, GermanyMVZ für Histologie, Zytologie und Molekulare Diagnostik Trier, 54296 Trier, GermanyAmong neonates, tested positive for SARS-CoV-2, the majority of infections occur through postpartum transmission. Only few reports describe intrauterine or intrapartum SARS-CoV-2 infections in newborns. To understand the route of transmission, detection of the virus or virus nucleic acid in the placenta and amniotic tissue are of special interest. Current methods to detect SARS-CoV-2 in placental tissue are immunohistochemistry, electron microscopy, in-situ hybridization, polymerase chain reaction (PCR) and next-generation sequencing. Recently, we described an alternative method for the detection of viral ribonucleic acid (RNA), by combination of reverse transcriptase-PCR and mass spectrometry (MS) in oropharyngeal and oral swabs. In this report, we could detect SARS-CoV-2 in formal-fixed and paraffin-embedded (FFPE) placental and amniotic tissue by multiplex RT-PCR MS. Additionally, we could identify the British variant (B.1.1.7) of the virus in this tissue by the same methodology. Combination of RT-PCR with MS is a fast and easy method to detect SARS-CoV-2 viral RNA, including specific variants in FFPE tissue.https://www.mdpi.com/1999-4915/14/3/604SARS-CoV-2FFPEmass spectrometryCOVID-19placenta |
| spellingShingle | Marina Wierz Beate Sauerbrei Petra Wandernoth Mark Kriegsmann Rita Casadonte Katharina Kriegsmann Jörg Kriegsmann Detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) including Variant Analysis by Mass Spectrometry in Placental Tissue Viruses SARS-CoV-2 FFPE mass spectrometry COVID-19 placenta |
| title | Detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) including Variant Analysis by Mass Spectrometry in Placental Tissue |
| title_full | Detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) including Variant Analysis by Mass Spectrometry in Placental Tissue |
| title_fullStr | Detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) including Variant Analysis by Mass Spectrometry in Placental Tissue |
| title_full_unstemmed | Detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) including Variant Analysis by Mass Spectrometry in Placental Tissue |
| title_short | Detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) including Variant Analysis by Mass Spectrometry in Placental Tissue |
| title_sort | detection of severe acute respiratory syndrome coronavirus 2 sars cov 2 including variant analysis by mass spectrometry in placental tissue |
| topic | SARS-CoV-2 FFPE mass spectrometry COVID-19 placenta |
| url | https://www.mdpi.com/1999-4915/14/3/604 |
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