Composition and Antifungal Activity of the Alkaloidal Fraction of <i>Lupinus mirabilis</i> Leaves: A Biochemometrics-Based Exploration
<i>Lupinus</i> plants are well-recognized due to their significant alkaloid content, which has made them the subject of several studies. However, the lack of chemical and biological information on the Colombian <i>Lupinus</i> species remains a fact. Therefore, the alkaloidal...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-04-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/9/2832 |
| _version_ | 1797503645172891648 |
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| author | Freddy A. Bernal Ericsson Coy-Barrera |
| author_facet | Freddy A. Bernal Ericsson Coy-Barrera |
| author_sort | Freddy A. Bernal |
| collection | DOAJ |
| description | <i>Lupinus</i> plants are well-recognized due to their significant alkaloid content, which has made them the subject of several studies. However, the lack of chemical and biological information on the Colombian <i>Lupinus</i> species remains a fact. Therefore, the alkaloidal fractions from the leaves of L. <i>mirabilis</i> obtained by conventional solvent and ultrasound-assisted extraction (CSE and UAE, respectively) at different time frames were analyzed. Sparteine (<b>2</b>) was the main component in all cases; however, its relative abundance showed large variability, ranging from 64.7% to 80.6%. Minor constituents were also affected by the extraction conditions. In general, prolonged times gave a higher proportion of alkaloids under CSE, while only a slight decrease was observed under UAE. Both the method and extraction time appeared to equally affect the ratios of particular alkaloids, leading to variations in their effect on the mycelial growth of <i>Fusarium oxysporum</i>. Holistic analysis through multiple-covariate statistical methods as an approach to integrating chemical and bioactivity datasets allowed inferring the compounds most likely responsible for the changes in mycelial growth inhibition. 13α-Hydroxylupanine (<b>12</b>) might represent a promising compound to be included in further studies against this phytopathogen. |
| first_indexed | 2024-03-10T03:53:39Z |
| format | Article |
| id | doaj.art-6c4bf6136053406ea433b90b03faa77a |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T03:53:39Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-6c4bf6136053406ea433b90b03faa77a2023-11-23T08:50:19ZengMDPI AGMolecules1420-30492022-04-01279283210.3390/molecules27092832Composition and Antifungal Activity of the Alkaloidal Fraction of <i>Lupinus mirabilis</i> Leaves: A Biochemometrics-Based ExplorationFreddy A. Bernal0Ericsson Coy-Barrera1Bioorganic Chemistry Laboratory, Facultad de Ciencias Básicas y Aplicadas, Universidad Militar Nueva Granada, Cajicá 250247, ColombiaBioorganic Chemistry Laboratory, Facultad de Ciencias Básicas y Aplicadas, Universidad Militar Nueva Granada, Cajicá 250247, Colombia<i>Lupinus</i> plants are well-recognized due to their significant alkaloid content, which has made them the subject of several studies. However, the lack of chemical and biological information on the Colombian <i>Lupinus</i> species remains a fact. Therefore, the alkaloidal fractions from the leaves of L. <i>mirabilis</i> obtained by conventional solvent and ultrasound-assisted extraction (CSE and UAE, respectively) at different time frames were analyzed. Sparteine (<b>2</b>) was the main component in all cases; however, its relative abundance showed large variability, ranging from 64.7% to 80.6%. Minor constituents were also affected by the extraction conditions. In general, prolonged times gave a higher proportion of alkaloids under CSE, while only a slight decrease was observed under UAE. Both the method and extraction time appeared to equally affect the ratios of particular alkaloids, leading to variations in their effect on the mycelial growth of <i>Fusarium oxysporum</i>. Holistic analysis through multiple-covariate statistical methods as an approach to integrating chemical and bioactivity datasets allowed inferring the compounds most likely responsible for the changes in mycelial growth inhibition. 13α-Hydroxylupanine (<b>12</b>) might represent a promising compound to be included in further studies against this phytopathogen.https://www.mdpi.com/1420-3049/27/9/2832<i>Lupinus mirabilis</i>quinolizidinesalkaloids<i>Fusarium oxysporum</i>ultrasound-assisted extractionantifungal activity |
| spellingShingle | Freddy A. Bernal Ericsson Coy-Barrera Composition and Antifungal Activity of the Alkaloidal Fraction of <i>Lupinus mirabilis</i> Leaves: A Biochemometrics-Based Exploration Molecules <i>Lupinus mirabilis</i> quinolizidines alkaloids <i>Fusarium oxysporum</i> ultrasound-assisted extraction antifungal activity |
| title | Composition and Antifungal Activity of the Alkaloidal Fraction of <i>Lupinus mirabilis</i> Leaves: A Biochemometrics-Based Exploration |
| title_full | Composition and Antifungal Activity of the Alkaloidal Fraction of <i>Lupinus mirabilis</i> Leaves: A Biochemometrics-Based Exploration |
| title_fullStr | Composition and Antifungal Activity of the Alkaloidal Fraction of <i>Lupinus mirabilis</i> Leaves: A Biochemometrics-Based Exploration |
| title_full_unstemmed | Composition and Antifungal Activity of the Alkaloidal Fraction of <i>Lupinus mirabilis</i> Leaves: A Biochemometrics-Based Exploration |
| title_short | Composition and Antifungal Activity of the Alkaloidal Fraction of <i>Lupinus mirabilis</i> Leaves: A Biochemometrics-Based Exploration |
| title_sort | composition and antifungal activity of the alkaloidal fraction of i lupinus mirabilis i leaves a biochemometrics based exploration |
| topic | <i>Lupinus mirabilis</i> quinolizidines alkaloids <i>Fusarium oxysporum</i> ultrasound-assisted extraction antifungal activity |
| url | https://www.mdpi.com/1420-3049/27/9/2832 |
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