Association of Melanocortin 4 Receptor gene polymorphism with growth traits in Bligon goat
Melanocortin 4 receptor gene is a potential marker gene for growth performance in ruminant and non-ruminant animals. The objective of this study was to perform allele and genotype frequencies, and association of melanocortin 4 receptor with growth traits in Bligon goat. Seventy seven blood samples w...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Diponegoro University, Faculty of Animal and Agricultural Sciences
2018-12-01
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| Series: | Journal of the Indonesian Tropical Animal Agriculture |
| Subjects: | |
| Online Access: | https://ejournal.undip.ac.id/index.php/jitaa/article/view/19304 |
| _version_ | 1828487844368220160 |
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| author | L. Latifah D. Maharani A. Kustantinah T. Hartatik |
| author_facet | L. Latifah D. Maharani A. Kustantinah T. Hartatik |
| author_sort | L. Latifah |
| collection | DOAJ |
| description | Melanocortin 4 receptor gene is a potential marker gene for growth performance in ruminant and non-ruminant animals. The objective of this study was to perform allele and genotype frequencies, and association of melanocortin 4 receptor with growth traits in Bligon goat. Seventy seven blood samples were collected and DNA isolated successfully. One pair primers were used for PCR of MC4R gene (F: 5’-TCGGGCGTCTTGTTCATCAT-3’ and R: 5’-CAAGACTGGGCACTGCTTCA-3’). Two SNPs (g.998A/G and g.1079C/T) were identified in exon of Bligon MC4R by DNA sequencing and PCR-RFLP. The SNP g.998A/G identified amino acid change from isoleucine to methionine (I-M), while the SNP g.1079C/T was synonymous polymorphism. Based on relationship analysis between Genotype of MC4R gene (SNP g.998A/G) and growth traits indicated that GG genotype was lowest in weaning weight (WW; AA=13.0±1.7; AG=11.2±3.6; GG=9.2±3.9), weaning body length (WBL; AA=46.8±2.4; AG=46.3±6.0; GG=43.8±5.3) and weaning chest circumference (WCC; AA=50.9±4.0; AG=50.7±4.6; GG=47.1±7.3) compare to AA and AG genotype (p<0.05). Individual with CC and CT genotypes at SNP g.1079C/T had higher (P<0.05) in WW (CC=12.6±2.1; CT=12.5±2.5) and average daily gain (ADG; CC=81.2±21.8; CT=79.3±26.2) than TT genotype (WW=10.6±3.1; ADG=58.3±30.5). This results suggested that SNP g.998A/G and g.1079C/T of MC4R gene could be used as the genetic marker of growth traits for selection in Bligon goat. |
| first_indexed | 2024-12-11T09:58:09Z |
| format | Article |
| id | doaj.art-6c4d945099c2453ea3badf83e743c0ad |
| institution | Directory Open Access Journal |
| issn | 2087-8273 2460-6278 |
| language | English |
| last_indexed | 2024-12-11T09:58:09Z |
| publishDate | 2018-12-01 |
| publisher | Diponegoro University, Faculty of Animal and Agricultural Sciences |
| record_format | Article |
| series | Journal of the Indonesian Tropical Animal Agriculture |
| spelling | doaj.art-6c4d945099c2453ea3badf83e743c0ad2022-12-22T01:12:12ZengDiponegoro University, Faculty of Animal and Agricultural SciencesJournal of the Indonesian Tropical Animal Agriculture2087-82732460-62782018-12-0143434335110.14710/jitaa.43.4.343-35114242Association of Melanocortin 4 Receptor gene polymorphism with growth traits in Bligon goatL. Latifah0D. Maharani1A. Kustantinah2T. Hartatik3Department of Animal Breeding and Reproduction, Faculty of Animal Science, Universitas Gadjah Mada Jl. Fauna No. 3, Sleman-YogyakartaDepartment of Animal Breeding and Reproduction, Faculty of Animal Science, Universitas Gadjah Mada Jl. Fauna No. 3, Sleman-YogyakartaDepartment of Animal Nutrition and Feed Science, Faculty of Animal Science, Universitas Gadjah Mada Jl. Fauna No. 3, Sleman-YogyakartaDepartment of Animal Breeding and Reproduction, Faculty of Animal Science, Universitas Gadjah Mada Jl. Fauna No. 3, Sleman-YogyakartaMelanocortin 4 receptor gene is a potential marker gene for growth performance in ruminant and non-ruminant animals. The objective of this study was to perform allele and genotype frequencies, and association of melanocortin 4 receptor with growth traits in Bligon goat. Seventy seven blood samples were collected and DNA isolated successfully. One pair primers were used for PCR of MC4R gene (F: 5’-TCGGGCGTCTTGTTCATCAT-3’ and R: 5’-CAAGACTGGGCACTGCTTCA-3’). Two SNPs (g.998A/G and g.1079C/T) were identified in exon of Bligon MC4R by DNA sequencing and PCR-RFLP. The SNP g.998A/G identified amino acid change from isoleucine to methionine (I-M), while the SNP g.1079C/T was synonymous polymorphism. Based on relationship analysis between Genotype of MC4R gene (SNP g.998A/G) and growth traits indicated that GG genotype was lowest in weaning weight (WW; AA=13.0±1.7; AG=11.2±3.6; GG=9.2±3.9), weaning body length (WBL; AA=46.8±2.4; AG=46.3±6.0; GG=43.8±5.3) and weaning chest circumference (WCC; AA=50.9±4.0; AG=50.7±4.6; GG=47.1±7.3) compare to AA and AG genotype (p<0.05). Individual with CC and CT genotypes at SNP g.1079C/T had higher (P<0.05) in WW (CC=12.6±2.1; CT=12.5±2.5) and average daily gain (ADG; CC=81.2±21.8; CT=79.3±26.2) than TT genotype (WW=10.6±3.1; ADG=58.3±30.5). This results suggested that SNP g.998A/G and g.1079C/T of MC4R gene could be used as the genetic marker of growth traits for selection in Bligon goat.https://ejournal.undip.ac.id/index.php/jitaa/article/view/19304BligongoatMC4RSNPgrowth traitsassociation |
| spellingShingle | L. Latifah D. Maharani A. Kustantinah T. Hartatik Association of Melanocortin 4 Receptor gene polymorphism with growth traits in Bligon goat Journal of the Indonesian Tropical Animal Agriculture Bligon goat MC4R SNP growth traits association |
| title | Association of Melanocortin 4 Receptor gene polymorphism with growth traits in Bligon goat |
| title_full | Association of Melanocortin 4 Receptor gene polymorphism with growth traits in Bligon goat |
| title_fullStr | Association of Melanocortin 4 Receptor gene polymorphism with growth traits in Bligon goat |
| title_full_unstemmed | Association of Melanocortin 4 Receptor gene polymorphism with growth traits in Bligon goat |
| title_short | Association of Melanocortin 4 Receptor gene polymorphism with growth traits in Bligon goat |
| title_sort | association of melanocortin 4 receptor gene polymorphism with growth traits in bligon goat |
| topic | Bligon goat MC4R SNP growth traits association |
| url | https://ejournal.undip.ac.id/index.php/jitaa/article/view/19304 |
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