Innate lymphoid cell markers: expression, localization, and regulation at the maternal-conceptus interface in pigs
Background: The regulation of maternal immunity is critical for the establishment and maintenance of successful pregnancy. Among many cell types regulating the immune system, innate lymphoid cells (ILCs) are known to play an important role in innate immunity. Although some reports show that ILCs are...
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| Format: | Article |
| Language: | English |
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The Korean Society of Animal Reproduction and Biotechnology
2023-09-01
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| Series: | Journal of Animal Reproduction and Biotechnology |
| Subjects: | |
| Online Access: | https://www.e-jarb.org/journal/view.html?uid=2659&vmd=Full |
| _version_ | 1797670103927488512 |
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| author | Yugyeong Cheon Inkyu Yoo Soohyung Lee Hakhyun Ka |
| author_facet | Yugyeong Cheon Inkyu Yoo Soohyung Lee Hakhyun Ka |
| author_sort | Yugyeong Cheon |
| collection | DOAJ |
| description | Background: The regulation of maternal immunity is critical for the establishment and maintenance of successful pregnancy. Among many cell types regulating the immune system, innate lymphoid cells (ILCs) are known to play an important role in innate immunity. Although some reports show that ILCs are present at the maternalconceptus interface in humans and mice, the expression and function of ILCs in the endometrium have not been studied in pigs.
Methods: Thus, we determined the expression, localization, and regulation of ILC markers, CD127 (a common marker for ILCs), BCL11B (a ILC2 marker), and RORC (a ILC3 marker) at the maternal-conceptus interface in pigs.
Results: The expression of BCL11B and RORC, but not CD127, in the endometrium changed during pregnancy in a stage-specific manner and the expression of CD127, BCL11B, and RORC was greatest on Day 15 during pregnancy. CD127, BCL11B, and RORC were also expressed in conceptus tissues during early pregnancy and in chorioallantoic tissues during the later stage of pregnancy. BCL11B and RORC proteins were localized to specific cells in endometrial stroma. The expression of CD127 and BCL11B, but not RORC, was increased by the increasing doses of interferon-γ (IFNG) in endometrial explants.
Conclusions: These results suggest that ILCs present at the maternal-conceptus interface may play a role in the establishment and maintenance of pregnancy by regulating the innate immunity in pigs. |
| first_indexed | 2024-03-11T20:54:53Z |
| format | Article |
| id | doaj.art-6c4de603214140c580004e9d911681f8 |
| institution | Directory Open Access Journal |
| issn | 2671-4639 2671-4663 |
| language | English |
| last_indexed | 2024-03-11T20:54:53Z |
| publishDate | 2023-09-01 |
| publisher | The Korean Society of Animal Reproduction and Biotechnology |
| record_format | Article |
| series | Journal of Animal Reproduction and Biotechnology |
| spelling | doaj.art-6c4de603214140c580004e9d911681f82023-09-30T14:21:35ZengThe Korean Society of Animal Reproduction and BiotechnologyJournal of Animal Reproduction and Biotechnology2671-46392671-46632023-09-01383899810.12750/JARB.38.3.89Innate lymphoid cell markers: expression, localization, and regulation at the maternal-conceptus interface in pigsYugyeong Cheon0https://orcid.org/0000-0002-9985-2013Inkyu Yoo1https://orcid.org/0000-0003-2766-0634Soohyung Lee2https://orcid.org/0000-0002-1952-263XHakhyun Ka3https://orcid.org/0000-0003-0213-5884Department of Biological Science and Technology, Yonsei University, Wonju 26493, KoreaDepartment of Biological Science and Technology, Yonsei University, Wonju 26493, KoreaDepartment of Biological Science and Technology, Yonsei University, Wonju 26493, KoreaDepartment of Biological Science and Technology, Yonsei University, Wonju 26493, KoreaBackground: The regulation of maternal immunity is critical for the establishment and maintenance of successful pregnancy. Among many cell types regulating the immune system, innate lymphoid cells (ILCs) are known to play an important role in innate immunity. Although some reports show that ILCs are present at the maternalconceptus interface in humans and mice, the expression and function of ILCs in the endometrium have not been studied in pigs. Methods: Thus, we determined the expression, localization, and regulation of ILC markers, CD127 (a common marker for ILCs), BCL11B (a ILC2 marker), and RORC (a ILC3 marker) at the maternal-conceptus interface in pigs. Results: The expression of BCL11B and RORC, but not CD127, in the endometrium changed during pregnancy in a stage-specific manner and the expression of CD127, BCL11B, and RORC was greatest on Day 15 during pregnancy. CD127, BCL11B, and RORC were also expressed in conceptus tissues during early pregnancy and in chorioallantoic tissues during the later stage of pregnancy. BCL11B and RORC proteins were localized to specific cells in endometrial stroma. The expression of CD127 and BCL11B, but not RORC, was increased by the increasing doses of interferon-γ (IFNG) in endometrial explants. Conclusions: These results suggest that ILCs present at the maternal-conceptus interface may play a role in the establishment and maintenance of pregnancy by regulating the innate immunity in pigs.https://www.e-jarb.org/journal/view.html?uid=2659&vmd=Fullendometriuminnate lymphoid cellpigpregnancy |
| spellingShingle | Yugyeong Cheon Inkyu Yoo Soohyung Lee Hakhyun Ka Innate lymphoid cell markers: expression, localization, and regulation at the maternal-conceptus interface in pigs Journal of Animal Reproduction and Biotechnology endometrium innate lymphoid cell pig pregnancy |
| title | Innate lymphoid cell markers: expression, localization, and regulation at the maternal-conceptus interface in pigs |
| title_full | Innate lymphoid cell markers: expression, localization, and regulation at the maternal-conceptus interface in pigs |
| title_fullStr | Innate lymphoid cell markers: expression, localization, and regulation at the maternal-conceptus interface in pigs |
| title_full_unstemmed | Innate lymphoid cell markers: expression, localization, and regulation at the maternal-conceptus interface in pigs |
| title_short | Innate lymphoid cell markers: expression, localization, and regulation at the maternal-conceptus interface in pigs |
| title_sort | innate lymphoid cell markers expression localization and regulation at the maternal conceptus interface in pigs |
| topic | endometrium innate lymphoid cell pig pregnancy |
| url | https://www.e-jarb.org/journal/view.html?uid=2659&vmd=Full |
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