Choline Acetyltransferase Induces the Functional Regeneration of the Salivary Gland in Aging SAMP1/Kl -/- Mice
Salivary gland dysfunction induces salivary flow reduction and a dry mouth, and commonly involves oral dysfunction, tooth structure deterioration, and infection through reduced salivation. This study aimed to investigate the impact of aging on the salivary gland by a metabolomics approach in an exte...
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MDPI AG
2021-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/1/404 |
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| author | Nguyen Khanh Toan Nguyen Chi Tai Soo-A Kim Sang-Gun Ahn |
| author_facet | Nguyen Khanh Toan Nguyen Chi Tai Soo-A Kim Sang-Gun Ahn |
| author_sort | Nguyen Khanh Toan |
| collection | DOAJ |
| description | Salivary gland dysfunction induces salivary flow reduction and a dry mouth, and commonly involves oral dysfunction, tooth structure deterioration, and infection through reduced salivation. This study aimed to investigate the impact of aging on the salivary gland by a metabolomics approach in an extensive aging mouse model, SAMP1/Klotho -/- mice. We found that the salivary secretion of SAMP1/Klotho -/- mice was dramatically decreased compared with that of SAMP1/Klotho WT (+/+) mice. Metabolomics profiling analysis showed that the level of acetylcholine was significantly decreased in SAMP1/Klotho -/- mice, although the corresponding levels of acetylcholine precursors, acetyl-CoA and choline, increased. Interestingly, the mRNA and protein expression of choline acetyltransferase (ChAT), which is responsible for catalyzing acetylcholine synthesis, was significantly decreased in SAMP1/Klotho -/- mice. The overexpression of ChAT induced the expression of salivary gland functional markers (α–amylase, ZO-1, and Aqua5) in primary cultured salivary gland cells from SAMP1/Klotho +/+ and -/- mice. In an in vivo study, adeno-associated virus (AAV)-ChAT transduction significantly increased saliva secretion compared with the control in SAMP1/Klotho -/- mice. These results suggest that the dysfunction in acetylcholine biosynthesis induced by ChAT reduction may cause impaired salivary gland function |
| first_indexed | 2024-03-10T13:32:56Z |
| format | Article |
| id | doaj.art-6c4e61cac5ae45ffa4338e1387547ef4 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T13:32:56Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-6c4e61cac5ae45ffa4338e1387547ef42023-11-21T07:46:30ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-01-0122140410.3390/ijms22010404Choline Acetyltransferase Induces the Functional Regeneration of the Salivary Gland in Aging SAMP1/Kl -/- MiceNguyen Khanh Toan0Nguyen Chi Tai1Soo-A Kim2Sang-Gun Ahn3Department of Pathology, School of Dentistry, Chosun University, Gwangju 61452, KoreaDepartment of Pathology, School of Dentistry, Chosun University, Gwangju 61452, KoreaDepartment of Biochemistry, School of Oriental Medicine, Dongguk University, Gyeongju 38066, KoreaDepartment of Pathology, School of Dentistry, Chosun University, Gwangju 61452, KoreaSalivary gland dysfunction induces salivary flow reduction and a dry mouth, and commonly involves oral dysfunction, tooth structure deterioration, and infection through reduced salivation. This study aimed to investigate the impact of aging on the salivary gland by a metabolomics approach in an extensive aging mouse model, SAMP1/Klotho -/- mice. We found that the salivary secretion of SAMP1/Klotho -/- mice was dramatically decreased compared with that of SAMP1/Klotho WT (+/+) mice. Metabolomics profiling analysis showed that the level of acetylcholine was significantly decreased in SAMP1/Klotho -/- mice, although the corresponding levels of acetylcholine precursors, acetyl-CoA and choline, increased. Interestingly, the mRNA and protein expression of choline acetyltransferase (ChAT), which is responsible for catalyzing acetylcholine synthesis, was significantly decreased in SAMP1/Klotho -/- mice. The overexpression of ChAT induced the expression of salivary gland functional markers (α–amylase, ZO-1, and Aqua5) in primary cultured salivary gland cells from SAMP1/Klotho +/+ and -/- mice. In an in vivo study, adeno-associated virus (AAV)-ChAT transduction significantly increased saliva secretion compared with the control in SAMP1/Klotho -/- mice. These results suggest that the dysfunction in acetylcholine biosynthesis induced by ChAT reduction may cause impaired salivary gland functionhttps://www.mdpi.com/1422-0067/22/1/404salivary gland dysfunctionSAMP1/KlothoCholine acetyltransferase (ChAT), Acetylcholine |
| spellingShingle | Nguyen Khanh Toan Nguyen Chi Tai Soo-A Kim Sang-Gun Ahn Choline Acetyltransferase Induces the Functional Regeneration of the Salivary Gland in Aging SAMP1/Kl -/- Mice International Journal of Molecular Sciences salivary gland dysfunction SAMP1/Klotho Choline acetyltransferase (ChAT), Acetylcholine |
| title | Choline Acetyltransferase Induces the Functional Regeneration of the Salivary Gland in Aging SAMP1/Kl -/- Mice |
| title_full | Choline Acetyltransferase Induces the Functional Regeneration of the Salivary Gland in Aging SAMP1/Kl -/- Mice |
| title_fullStr | Choline Acetyltransferase Induces the Functional Regeneration of the Salivary Gland in Aging SAMP1/Kl -/- Mice |
| title_full_unstemmed | Choline Acetyltransferase Induces the Functional Regeneration of the Salivary Gland in Aging SAMP1/Kl -/- Mice |
| title_short | Choline Acetyltransferase Induces the Functional Regeneration of the Salivary Gland in Aging SAMP1/Kl -/- Mice |
| title_sort | choline acetyltransferase induces the functional regeneration of the salivary gland in aging samp1 kl mice |
| topic | salivary gland dysfunction SAMP1/Klotho Choline acetyltransferase (ChAT), Acetylcholine |
| url | https://www.mdpi.com/1422-0067/22/1/404 |
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