Detection of Respiratory Syncytial Virus using Direct Fluorescent Antibody Assay in Paediatric Patients with Acute Respiratory Tract Infection
Introduction: Severe Respiratory Syncytial Virus (RSV) pulmonary disease manifesting as bronchiolitis and pneumonia continues to play a major role in the childhood mortality and morbidity. Hence the present study was undertaken to evaluate the prevalence of RSV among hospitalized children presen...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2016-06-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/8044/17530_CE(RA1)_F(T)_PF1(ROAK)_PFA(NC_AK)_PF2(PAG).pdf |
| Summary: | Introduction: Severe Respiratory Syncytial Virus (RSV)
pulmonary disease manifesting as bronchiolitis and pneumonia
continues to play a major role in the childhood mortality and
morbidity. Hence the present study was undertaken to evaluate
the prevalence of RSV among hospitalized children presenting
with Acute Respiratory Tract Infection (ARTI) and its correlation
with risk factors.
Aim: To determine the occurrence of RSV related respiratory tract
infection in paediatric patients and to access the risk factors and
clinical features associated.
Materials and Methods: RSV antigen detection was performed
by Direct Fluorescent Antibody (DFA) staining on 100
nasopharyngeal aspirate collected from hospitalized children
below 5 years of age with a diagnosis of ARTI.
Results: Out of the 100 samples tested for RSV with DFA, 22
(22%) were found RSV positive with a mean age of 12 months
and a male to female ratio of (1.75:1). Clinical features significantly
associated with RSV were wheezing and breathlessness.
Congenital heart disease (CHD) and prematurity were the risk
factors significantly associated with RSV infection.
Conclusion: RSV infection is a significant cause of morbidity
among children presenting with ARTI. In resource limited countries
DFA can be used as an important tool for rapid detection of
RSV and can potentially eliminate prolonged hospitalization and
unnecessary use of antibiotics. |
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| ISSN: | 2249-782X 0973-709X |