IgG N-Glycosylation Is Altered in Coronary Artery Disease
Coronary artery disease (CAD) is the most common cardiovascular disease (CVD), and previous studies have shown a significant association between N-glycosylation, a highly regulated posttranslational modification, and the development of atherosclerotic plaques. Our aim was to determine whether the N-...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/13/2/375 |
| _version_ | 1797622067655344128 |
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| author | Barbara Radovani Frano Vučković Aldo P. Maggioni Ele Ferrannini Gordan Lauc Ivan Gudelj |
| author_facet | Barbara Radovani Frano Vučković Aldo P. Maggioni Ele Ferrannini Gordan Lauc Ivan Gudelj |
| author_sort | Barbara Radovani |
| collection | DOAJ |
| description | Coronary artery disease (CAD) is the most common cardiovascular disease (CVD), and previous studies have shown a significant association between N-glycosylation, a highly regulated posttranslational modification, and the development of atherosclerotic plaques. Our aim was to determine whether the N-glycome of immunoglobulin G (IgG) is associated with CAD, as N-glycans are known to alter the effector functions of IgG, which may enhance the inflammatory response in CAD. Therefore, in this study, we isolated IgG from subjects with coronary atherosclerosis (CAD+) and from subjects with clean coronaries (CAD−). The purified IgGs were denatured and enzymatically deglycosylated, and the released and fluorescently labelled N-glycans were analysed by ultra-high performance liquid chromatography based on hydrophilic interactions with fluorescence detection (HILIC-UHPLC-FLR). Sex-stratified analysis of 316 CAD− and 156 CAD+ cases revealed differences in IgG N-glycome composition. The most notable differences were observed in women, where the presence of sialylated N-glycan structures was negatively associated with CAD. The obtained chromatograms provide insight into the IgG N-glycome composition in CAD as well as the biomarker potential of IgG N-glycans in CAD. |
| first_indexed | 2024-03-11T09:05:58Z |
| format | Article |
| id | doaj.art-6c4ed80f61e9481bb5b184d4a3ef8efe |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-11T09:05:58Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-6c4ed80f61e9481bb5b184d4a3ef8efe2023-11-16T19:24:19ZengMDPI AGBiomolecules2218-273X2023-02-0113237510.3390/biom13020375IgG N-Glycosylation Is Altered in Coronary Artery DiseaseBarbara Radovani0Frano Vučković1Aldo P. Maggioni2Ele Ferrannini3Gordan Lauc4Ivan Gudelj5Department of Biotechnology, University of Rijeka, 51000 Rijeka, CroatiaGenos Glycoscience Research Laboratory, 10000 Zagreb, CroatiaHeart Care Foundation ANMCO Research Center, 50100 Florence, ItalyCNR Institute of Clinical Physiology, 56100 Pisa, ItalyGenos Glycoscience Research Laboratory, 10000 Zagreb, CroatiaDepartment of Biotechnology, University of Rijeka, 51000 Rijeka, CroatiaCoronary artery disease (CAD) is the most common cardiovascular disease (CVD), and previous studies have shown a significant association between N-glycosylation, a highly regulated posttranslational modification, and the development of atherosclerotic plaques. Our aim was to determine whether the N-glycome of immunoglobulin G (IgG) is associated with CAD, as N-glycans are known to alter the effector functions of IgG, which may enhance the inflammatory response in CAD. Therefore, in this study, we isolated IgG from subjects with coronary atherosclerosis (CAD+) and from subjects with clean coronaries (CAD−). The purified IgGs were denatured and enzymatically deglycosylated, and the released and fluorescently labelled N-glycans were analysed by ultra-high performance liquid chromatography based on hydrophilic interactions with fluorescence detection (HILIC-UHPLC-FLR). Sex-stratified analysis of 316 CAD− and 156 CAD+ cases revealed differences in IgG N-glycome composition. The most notable differences were observed in women, where the presence of sialylated N-glycan structures was negatively associated with CAD. The obtained chromatograms provide insight into the IgG N-glycome composition in CAD as well as the biomarker potential of IgG N-glycans in CAD.https://www.mdpi.com/2218-273X/13/2/375immunoglobulin GN-glycansglycosylationinflammationcoronary artery diseasecardiovascular disease |
| spellingShingle | Barbara Radovani Frano Vučković Aldo P. Maggioni Ele Ferrannini Gordan Lauc Ivan Gudelj IgG N-Glycosylation Is Altered in Coronary Artery Disease Biomolecules immunoglobulin G N-glycans glycosylation inflammation coronary artery disease cardiovascular disease |
| title | IgG N-Glycosylation Is Altered in Coronary Artery Disease |
| title_full | IgG N-Glycosylation Is Altered in Coronary Artery Disease |
| title_fullStr | IgG N-Glycosylation Is Altered in Coronary Artery Disease |
| title_full_unstemmed | IgG N-Glycosylation Is Altered in Coronary Artery Disease |
| title_short | IgG N-Glycosylation Is Altered in Coronary Artery Disease |
| title_sort | igg n glycosylation is altered in coronary artery disease |
| topic | immunoglobulin G N-glycans glycosylation inflammation coronary artery disease cardiovascular disease |
| url | https://www.mdpi.com/2218-273X/13/2/375 |
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