The pulsed light inactivation of veterinary relevant microbial biofilms and the use of a RTPCR assay to detect parasite species within biofilm structures
The presence of pathogenic organisms namely parasite species and bacteria in biofilms in veterinary settings, is a public health concern in relation to human and animal exposure. Veterinary clinics represent a significant risk factor for the transfer of pathogens from housed animals to humans, espec...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Tripoli University
2016-01-01
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| Series: | Open Veterinary Journal |
| Subjects: | |
| Online Access: | http://www.openveterinaryjournal.com/2016/Volume%206%20%281%29/OVJ-144-10-15%20M.%20Garvey%20et%20al.pdf |
| _version_ | 1818677106003935232 |
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| author | M. Garvey G. Coughlan N. Murphy N. Rowan |
| author_facet | M. Garvey G. Coughlan N. Murphy N. Rowan |
| author_sort | M. Garvey |
| collection | DOAJ |
| description | The presence of pathogenic organisms namely parasite species and bacteria in biofilms in veterinary settings, is a public health concern in relation to human and animal exposure. Veterinary clinics represent a significant risk factor for the transfer of pathogens from housed animals to humans, especially in cases of wound infection and the shedding of faecal matter. This study aims to provide a means of detecting veterinary relevant parasite species in bacterial biofilms, and to provide a means of disinfecting these biofilms. A real time PCR assay was utilized to detect parasite DNA in Bacillus cereus biofilms on stainless steel and PVC surfaces. Results show that both Cryptosporidium and Giardia attach to biofilms in large numbers (100-1000 oo/cysts) in as little as 72 hours. Pulsed light successfully inactivated all test species (Listeria, Salmonella, Bacillus, Escherichia) in planktonic and biofilm form with an increase in inactivation for every increase in UV dose. |
| first_indexed | 2024-12-17T08:54:05Z |
| format | Article |
| id | doaj.art-6c57280432404f93b54997abb65d2ed2 |
| institution | Directory Open Access Journal |
| issn | 2218-6050 2218-6050 |
| language | English |
| last_indexed | 2024-12-17T08:54:05Z |
| publishDate | 2016-01-01 |
| publisher | Tripoli University |
| record_format | Article |
| series | Open Veterinary Journal |
| spelling | doaj.art-6c57280432404f93b54997abb65d2ed22022-12-21T21:56:00ZengTripoli UniversityOpen Veterinary Journal2218-60502218-60502016-01-01611522The pulsed light inactivation of veterinary relevant microbial biofilms and the use of a RTPCR assay to detect parasite species within biofilm structuresM. Garvey0G. Coughlan1N. Murphy2N. Rowan3Department of Life Sciences, Institute of Technology Sligo, Sligo, IrelandDepartment of Parasitology, National University of Ireland Maynooth, Maynooth, Ireland and Bioscience Research Institute, Athlone Institute of Technology, Athlone, IrelandDepartment of Parasitology, National University of Ireland Maynooth, Maynooth, IrelandBioscience Research Institute, Athlone Institute of Technology, Athlone, IrelandThe presence of pathogenic organisms namely parasite species and bacteria in biofilms in veterinary settings, is a public health concern in relation to human and animal exposure. Veterinary clinics represent a significant risk factor for the transfer of pathogens from housed animals to humans, especially in cases of wound infection and the shedding of faecal matter. This study aims to provide a means of detecting veterinary relevant parasite species in bacterial biofilms, and to provide a means of disinfecting these biofilms. A real time PCR assay was utilized to detect parasite DNA in Bacillus cereus biofilms on stainless steel and PVC surfaces. Results show that both Cryptosporidium and Giardia attach to biofilms in large numbers (100-1000 oo/cysts) in as little as 72 hours. Pulsed light successfully inactivated all test species (Listeria, Salmonella, Bacillus, Escherichia) in planktonic and biofilm form with an increase in inactivation for every increase in UV dose.http://www.openveterinaryjournal.com/2016/Volume%206%20%281%29/OVJ-144-10-15%20M.%20Garvey%20et%20al.pdfBiofilmsCryptosporidiumGiardiaPCRVeterinary |
| spellingShingle | M. Garvey G. Coughlan N. Murphy N. Rowan The pulsed light inactivation of veterinary relevant microbial biofilms and the use of a RTPCR assay to detect parasite species within biofilm structures Open Veterinary Journal Biofilms Cryptosporidium Giardia PCR Veterinary |
| title | The pulsed light inactivation of veterinary relevant microbial biofilms and the use of a RTPCR assay to detect parasite species within biofilm structures |
| title_full | The pulsed light inactivation of veterinary relevant microbial biofilms and the use of a RTPCR assay to detect parasite species within biofilm structures |
| title_fullStr | The pulsed light inactivation of veterinary relevant microbial biofilms and the use of a RTPCR assay to detect parasite species within biofilm structures |
| title_full_unstemmed | The pulsed light inactivation of veterinary relevant microbial biofilms and the use of a RTPCR assay to detect parasite species within biofilm structures |
| title_short | The pulsed light inactivation of veterinary relevant microbial biofilms and the use of a RTPCR assay to detect parasite species within biofilm structures |
| title_sort | pulsed light inactivation of veterinary relevant microbial biofilms and the use of a rtpcr assay to detect parasite species within biofilm structures |
| topic | Biofilms Cryptosporidium Giardia PCR Veterinary |
| url | http://www.openveterinaryjournal.com/2016/Volume%206%20%281%29/OVJ-144-10-15%20M.%20Garvey%20et%20al.pdf |
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