Evolution in the Test-Tube as a Means to Create Selective Biocatalysts
The development of chiral catalysts for use in synthetic organic chemistry is traditionally associated with progress in asymmetric transition metal catalysis or organocatalysis. In addition to these options chemists have employed enzymes for a number of asymmetric transformations for a lon...
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| Format: | Article |
| Language: | deu |
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Swiss Chemical Society
2007-03-01
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| Series: | CHIMIA |
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| Online Access: | https://chimia.ch/chimia/article/view/4284 |
| _version_ | 1797987501145587712 |
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| author | Manfred T. Reetz |
| author_facet | Manfred T. Reetz |
| author_sort | Manfred T. Reetz |
| collection | DOAJ |
| description |
The development of chiral catalysts for use in synthetic organic chemistry is traditionally associated with progress in asymmetric transition metal catalysis or organocatalysis. In addition to these options chemists have employed enzymes for a number of asymmetric transformations for
a long time. However, limited substrate acceptance and insufficient enantioselectivity are the crucial factors which prevent the general exploitation of biocatalysis in organic chemistry. In order to solve this long-standing problem, we proposed some time ago the concept of directed evolution
of enantioselective enzymes. This report constitutes a short overview of the principle involved, together with a description of the first examples and the illumination of the present and future challenges.
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| first_indexed | 2024-04-11T07:49:29Z |
| format | Article |
| id | doaj.art-6c58fe1a42d4450fbb63e6cf68e5acf3 |
| institution | Directory Open Access Journal |
| issn | 0009-4293 2673-2424 |
| language | deu |
| last_indexed | 2024-04-11T07:49:29Z |
| publishDate | 2007-03-01 |
| publisher | Swiss Chemical Society |
| record_format | Article |
| series | CHIMIA |
| spelling | doaj.art-6c58fe1a42d4450fbb63e6cf68e5acf32022-12-22T04:36:08ZdeuSwiss Chemical SocietyCHIMIA0009-42932673-24242007-03-0161310.2533/chimia.2007.100Evolution in the Test-Tube as a Means to Create Selective BiocatalystsManfred T. Reetz The development of chiral catalysts for use in synthetic organic chemistry is traditionally associated with progress in asymmetric transition metal catalysis or organocatalysis. In addition to these options chemists have employed enzymes for a number of asymmetric transformations for a long time. However, limited substrate acceptance and insufficient enantioselectivity are the crucial factors which prevent the general exploitation of biocatalysis in organic chemistry. In order to solve this long-standing problem, we proposed some time ago the concept of directed evolution of enantioselective enzymes. This report constitutes a short overview of the principle involved, together with a description of the first examples and the illumination of the present and future challenges. https://chimia.ch/chimia/article/view/4284Asymmetric catalysisDirected evolutionEnantioselectivityEnzymesGene mutagenesisHigh-throughput screening |
| spellingShingle | Manfred T. Reetz Evolution in the Test-Tube as a Means to Create Selective Biocatalysts CHIMIA Asymmetric catalysis Directed evolution Enantioselectivity Enzymes Gene mutagenesis High-throughput screening |
| title | Evolution in the Test-Tube as a Means to Create Selective Biocatalysts |
| title_full | Evolution in the Test-Tube as a Means to Create Selective Biocatalysts |
| title_fullStr | Evolution in the Test-Tube as a Means to Create Selective Biocatalysts |
| title_full_unstemmed | Evolution in the Test-Tube as a Means to Create Selective Biocatalysts |
| title_short | Evolution in the Test-Tube as a Means to Create Selective Biocatalysts |
| title_sort | evolution in the test tube as a means to create selective biocatalysts |
| topic | Asymmetric catalysis Directed evolution Enantioselectivity Enzymes Gene mutagenesis High-throughput screening |
| url | https://chimia.ch/chimia/article/view/4284 |
| work_keys_str_mv | AT manfredtreetz evolutioninthetesttubeasameanstocreateselectivebiocatalysts |