Evaluation of enterotoxigenic Bacteroides fragilis correlation with the expression of cellular signaling pathway genes in Iranian patients with colorectal cancer
Abstract Background Colorectal cancer (CRC) is one of the most common cancers all over the world, and dysbiosis in the gut microbiota may play a role in colorectal carcinogenesis. Bacteroides fragilis can lead to tumorigenesis by changing signaling pathways, including the WNT/β-catenin pathway. Ther...
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| Format: | Article |
| Language: | English |
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BMC
2023-08-01
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| Series: | Infectious Agents and Cancer |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13027-023-00523-w |
| _version_ | 1827724482412806144 |
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| author | Leila Dadgar-Zankbar Aref Shariati Narjess Bostanghadiri Zahra Elahi Shiva Mirkalantari Shabnam Razavi Fatemeh Kamali Davood Darban-Sarokhalil |
| author_facet | Leila Dadgar-Zankbar Aref Shariati Narjess Bostanghadiri Zahra Elahi Shiva Mirkalantari Shabnam Razavi Fatemeh Kamali Davood Darban-Sarokhalil |
| author_sort | Leila Dadgar-Zankbar |
| collection | DOAJ |
| description | Abstract Background Colorectal cancer (CRC) is one of the most common cancers all over the world, and dysbiosis in the gut microbiota may play a role in colorectal carcinogenesis. Bacteroides fragilis can lead to tumorigenesis by changing signaling pathways, including the WNT/β-catenin pathway. Therefore, in the present study, we investigated the correlation between the enterotoxigenic B. fragilis amount and the expression of signaling pathway genes involved in CRC. Materials and methods B. fragilis was determined in 30 tumors and adjacent healthy tissues by the qPCR method. Next, the relationship between enterotoxigenic B. fragilis and the expression of signaling pathway genes, including CCND1, TP53, BCL2, BAX, WNT, TCF, AXIN, APC, and CTNNB1 was investigated. Additionally, possible correlations between clinicopathological features of the tumor samples and the abundance of B. fragilis were analyzed. Results The results showed that B. fragilis was detected in 100% of tumor samples and 86% of healthy tissues. Additionally, enterotoxigenic B. fragilis colonized 47% of all samples, and bft-1 toxin was the most frequently found isotype among the samples. The analysis showed that the high level of B. fragilis has a significant relationship with the high expression of AXIN, CTNNB1, and BCL2 genes. On the other hand, our results did not show any possible correlation between this bacterium and the clinicopathological features of the tumor sample. Conclusion B. fragilis had a higher abundance in the tumor samples than in healthy tissues, and this bacterium may lead to CRC by making changes in cellular signaling pathways and genes. Therefore, to better understand the physiological effects of B. fragilis on the inflammatory response and CRC, future research should focus on dissecting the molecular mechanisms by which this bacterium regulates cellular signaling pathways. |
| first_indexed | 2024-03-10T22:16:25Z |
| format | Article |
| id | doaj.art-6c5ed0454dbc4b849cbad9bc78318da9 |
| institution | Directory Open Access Journal |
| issn | 1750-9378 |
| language | English |
| last_indexed | 2024-03-10T22:16:25Z |
| publishDate | 2023-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Infectious Agents and Cancer |
| spelling | doaj.art-6c5ed0454dbc4b849cbad9bc78318da92023-11-19T12:25:41ZengBMCInfectious Agents and Cancer1750-93782023-08-0118111010.1186/s13027-023-00523-wEvaluation of enterotoxigenic Bacteroides fragilis correlation with the expression of cellular signaling pathway genes in Iranian patients with colorectal cancerLeila Dadgar-Zankbar0Aref Shariati1Narjess Bostanghadiri2Zahra Elahi3Shiva Mirkalantari4Shabnam Razavi5Fatemeh Kamali6Davood Darban-Sarokhalil7Department of Microbiology, School of Medicine, Iran University of Medical SciencesMolecular and Medicine Research Center, Khomein University of Medical SciencesDepartment of Microbiology, School of Medicine, Iran University of Medical SciencesDepartment of Microbiology, School of Medicine, Iran University of Medical SciencesDepartment of Microbiology, School of Medicine, Iran University of Medical SciencesDepartment of Microbiology, School of Medicine, Iran University of Medical SciencesIran National Tumor Bank, Cancer Institute of Iran, Tehran University of Medical SciencesDepartment of Microbiology, School of Medicine, Iran University of Medical SciencesAbstract Background Colorectal cancer (CRC) is one of the most common cancers all over the world, and dysbiosis in the gut microbiota may play a role in colorectal carcinogenesis. Bacteroides fragilis can lead to tumorigenesis by changing signaling pathways, including the WNT/β-catenin pathway. Therefore, in the present study, we investigated the correlation between the enterotoxigenic B. fragilis amount and the expression of signaling pathway genes involved in CRC. Materials and methods B. fragilis was determined in 30 tumors and adjacent healthy tissues by the qPCR method. Next, the relationship between enterotoxigenic B. fragilis and the expression of signaling pathway genes, including CCND1, TP53, BCL2, BAX, WNT, TCF, AXIN, APC, and CTNNB1 was investigated. Additionally, possible correlations between clinicopathological features of the tumor samples and the abundance of B. fragilis were analyzed. Results The results showed that B. fragilis was detected in 100% of tumor samples and 86% of healthy tissues. Additionally, enterotoxigenic B. fragilis colonized 47% of all samples, and bft-1 toxin was the most frequently found isotype among the samples. The analysis showed that the high level of B. fragilis has a significant relationship with the high expression of AXIN, CTNNB1, and BCL2 genes. On the other hand, our results did not show any possible correlation between this bacterium and the clinicopathological features of the tumor sample. Conclusion B. fragilis had a higher abundance in the tumor samples than in healthy tissues, and this bacterium may lead to CRC by making changes in cellular signaling pathways and genes. Therefore, to better understand the physiological effects of B. fragilis on the inflammatory response and CRC, future research should focus on dissecting the molecular mechanisms by which this bacterium regulates cellular signaling pathways.https://doi.org/10.1186/s13027-023-00523-wBacteroides fragilisColorectal cancerWNT/β-cateninTP53BCL2 |
| spellingShingle | Leila Dadgar-Zankbar Aref Shariati Narjess Bostanghadiri Zahra Elahi Shiva Mirkalantari Shabnam Razavi Fatemeh Kamali Davood Darban-Sarokhalil Evaluation of enterotoxigenic Bacteroides fragilis correlation with the expression of cellular signaling pathway genes in Iranian patients with colorectal cancer Infectious Agents and Cancer Bacteroides fragilis Colorectal cancer WNT/β-catenin TP53 BCL2 |
| title | Evaluation of enterotoxigenic Bacteroides fragilis correlation with the expression of cellular signaling pathway genes in Iranian patients with colorectal cancer |
| title_full | Evaluation of enterotoxigenic Bacteroides fragilis correlation with the expression of cellular signaling pathway genes in Iranian patients with colorectal cancer |
| title_fullStr | Evaluation of enterotoxigenic Bacteroides fragilis correlation with the expression of cellular signaling pathway genes in Iranian patients with colorectal cancer |
| title_full_unstemmed | Evaluation of enterotoxigenic Bacteroides fragilis correlation with the expression of cellular signaling pathway genes in Iranian patients with colorectal cancer |
| title_short | Evaluation of enterotoxigenic Bacteroides fragilis correlation with the expression of cellular signaling pathway genes in Iranian patients with colorectal cancer |
| title_sort | evaluation of enterotoxigenic bacteroides fragilis correlation with the expression of cellular signaling pathway genes in iranian patients with colorectal cancer |
| topic | Bacteroides fragilis Colorectal cancer WNT/β-catenin TP53 BCL2 |
| url | https://doi.org/10.1186/s13027-023-00523-w |
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