Antibiotic-induced gut microbiota dysbiosis has a functional impact on purine metabolism
Abstract Background Dysbiosis of the gut microbiota is closely linked to hyperuricemia. However, the effect of the microbiome on uric acid (UA) metabolism remains unclear. This study aimed to explore the mechanisms through which microbiomes affect UA metabolism with the hypothesis that modifying the...
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| Format: | Article |
| Language: | English |
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BMC
2023-07-01
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| Series: | BMC Microbiology |
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| Online Access: | https://doi.org/10.1186/s12866-023-02932-8 |
| _version_ | 1797779055717646336 |
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| author | Xin Liu Leyong Ke Ke Lei Qian Yu Wenqing Zhang Changgui Li Zibin Tian |
| author_facet | Xin Liu Leyong Ke Ke Lei Qian Yu Wenqing Zhang Changgui Li Zibin Tian |
| author_sort | Xin Liu |
| collection | DOAJ |
| description | Abstract Background Dysbiosis of the gut microbiota is closely linked to hyperuricemia. However, the effect of the microbiome on uric acid (UA) metabolism remains unclear. This study aimed to explore the mechanisms through which microbiomes affect UA metabolism with the hypothesis that modifying the intestinal microbiota influences the development of hyperuricemia. Results We proposed combining an antibiotic strategy with protein-protein interaction analysis to test this hypothesis. The data demonstrated that antibiotics altered the composition of gut microbiota as UA increased, and that the spectrum of the antibiotic was connected to the purine salvage pathway. The antibiotic-elevated UA concentration was dependent on the increase in microbiomes that code for the proteins involved in purine metabolism, and was paralleled by the depletion of bacteria-coding enzymes required for the purine salvage pathway. On the contrary, the microbiota with abundant purine salvage proteins decreased hyperuricemia. We also found that the antibiotic-increased microbiota coincided with a higher relative abundance of bacteria in hyperuricemia mice. Conclusions An antibiotic strategy combined with the prediction of microbiome bacterial function presents a feasible method for defining the key bacteria involved in hyperuricemia. Our investigations discovered that the core microbiomes of hyperuricemia may be related to the gut microbiota that enriches purine metabolism related-proteins. However, the bacteria that enrich the purine salvage-proteins may be a probiotic for decreasing urate, and are more likely to be killed by antibiotics. Therefore, the purine salvage pathway may be a potential target for the treatment of both hyperuricemia and antibiotic resistance. |
| first_indexed | 2024-03-12T23:25:13Z |
| format | Article |
| id | doaj.art-6c607fb7eba24d8b8826dbcfefa18327 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-03-12T23:25:13Z |
| publishDate | 2023-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-6c607fb7eba24d8b8826dbcfefa183272023-07-16T11:11:06ZengBMCBMC Microbiology1471-21802023-07-0123111710.1186/s12866-023-02932-8Antibiotic-induced gut microbiota dysbiosis has a functional impact on purine metabolismXin Liu0Leyong Ke1Ke Lei2Qian Yu3Wenqing Zhang4Changgui Li5Zibin Tian6Department of Gastroenterology, The Affiliated Hospital of Qingdao UniversityDepartment of Cosmetic surgery, Kunming Medical UniversityCenter of Tumor Immunology and Cytotherapy, Medical Research Center, The Affiliated Hospital of Qingdao UniversityCenter of Tumor Immunology and Cytotherapy, Medical Research Center, The Affiliated Hospital of Qingdao UniversityDepartment of Gastroenterology, The Affiliated Hospital of Qingdao UniversityInstitute of Metabolic Diseases, Qingdao UniversityDepartment of Gastroenterology, The Affiliated Hospital of Qingdao UniversityAbstract Background Dysbiosis of the gut microbiota is closely linked to hyperuricemia. However, the effect of the microbiome on uric acid (UA) metabolism remains unclear. This study aimed to explore the mechanisms through which microbiomes affect UA metabolism with the hypothesis that modifying the intestinal microbiota influences the development of hyperuricemia. Results We proposed combining an antibiotic strategy with protein-protein interaction analysis to test this hypothesis. The data demonstrated that antibiotics altered the composition of gut microbiota as UA increased, and that the spectrum of the antibiotic was connected to the purine salvage pathway. The antibiotic-elevated UA concentration was dependent on the increase in microbiomes that code for the proteins involved in purine metabolism, and was paralleled by the depletion of bacteria-coding enzymes required for the purine salvage pathway. On the contrary, the microbiota with abundant purine salvage proteins decreased hyperuricemia. We also found that the antibiotic-increased microbiota coincided with a higher relative abundance of bacteria in hyperuricemia mice. Conclusions An antibiotic strategy combined with the prediction of microbiome bacterial function presents a feasible method for defining the key bacteria involved in hyperuricemia. Our investigations discovered that the core microbiomes of hyperuricemia may be related to the gut microbiota that enriches purine metabolism related-proteins. However, the bacteria that enrich the purine salvage-proteins may be a probiotic for decreasing urate, and are more likely to be killed by antibiotics. Therefore, the purine salvage pathway may be a potential target for the treatment of both hyperuricemia and antibiotic resistance.https://doi.org/10.1186/s12866-023-02932-8Gut microbiotaAntibioticPurine metabolismPurine salvage pathway |
| spellingShingle | Xin Liu Leyong Ke Ke Lei Qian Yu Wenqing Zhang Changgui Li Zibin Tian Antibiotic-induced gut microbiota dysbiosis has a functional impact on purine metabolism BMC Microbiology Gut microbiota Antibiotic Purine metabolism Purine salvage pathway |
| title | Antibiotic-induced gut microbiota dysbiosis has a functional impact on purine metabolism |
| title_full | Antibiotic-induced gut microbiota dysbiosis has a functional impact on purine metabolism |
| title_fullStr | Antibiotic-induced gut microbiota dysbiosis has a functional impact on purine metabolism |
| title_full_unstemmed | Antibiotic-induced gut microbiota dysbiosis has a functional impact on purine metabolism |
| title_short | Antibiotic-induced gut microbiota dysbiosis has a functional impact on purine metabolism |
| title_sort | antibiotic induced gut microbiota dysbiosis has a functional impact on purine metabolism |
| topic | Gut microbiota Antibiotic Purine metabolism Purine salvage pathway |
| url | https://doi.org/10.1186/s12866-023-02932-8 |
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