Oligochitosan glycosylation of casein by TGase: Protective effect and mechanism on alcohol-induced intestinal injury in C57BL/6 mice
Glycosylated protein by transglutaminase-type could alter the structures and functional properties of proteins. However, the beneficial effects of glycosylated proteins against alcohol-induced intestinal injury remain unclear. Herein, the protective role and mechanism of glycosylated casein ameliora...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464623004929 |
| _version_ | 1797435803791523840 |
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| author | Yu Wang Yingying Yue Huimin Wang Yujun Jiang Jia Shi |
| author_facet | Yu Wang Yingying Yue Huimin Wang Yujun Jiang Jia Shi |
| author_sort | Yu Wang |
| collection | DOAJ |
| description | Glycosylated protein by transglutaminase-type could alter the structures and functional properties of proteins. However, the beneficial effects of glycosylated proteins against alcohol-induced intestinal injury remain unclear. Herein, the protective role and mechanism of glycosylated casein ameliorating alcohol-induced intestinal injury were assessed in vivo. The results showed that glycosylated casein and casein significantly relieved alcohol-induced oxidative stress and inflammatory responses in a concentration-dependent manner. The interventions of glycosylated casein and casein decreased serum parameters, improved the activities of antioxidant enzymes, reduced levels of inflammatory cytokines, and alleviated histopathological impairment in intestine tissues. Glycosylated casein and casein treatment downregulated protein expressions of TLR4, NF-κB p65, IKB-α, and MyD88 in mice, which activated the TLR4/NF-κB signaling pathway as a protective mechanism. More importantly, glycosylated caseinate showed a better effect against alcohol-induced intestinal injury than casein, suggesting that transglutaminase-mediated glycosylation of proteins is a useful technique to enforce protein biofunctions in the intestine. |
| first_indexed | 2024-03-09T10:53:22Z |
| format | Article |
| id | doaj.art-6c6416c99a3d4f24bb88c81ce92aef8d |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-03-09T10:53:22Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-6c6416c99a3d4f24bb88c81ce92aef8d2023-12-01T05:01:12ZengElsevierJournal of Functional Foods1756-46462023-12-01111105892Oligochitosan glycosylation of casein by TGase: Protective effect and mechanism on alcohol-induced intestinal injury in C57BL/6 miceYu Wang0Yingying Yue1Huimin Wang2Yujun Jiang3Jia Shi4Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, PR ChinaKey Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, PR ChinaKey Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, PR ChinaKey Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, PR China; Department of Food Science, Northeast Agricultural University, Harbin 150030, PR ChinaKey Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, PR China; Department of Food Science, Northeast Agricultural University, Harbin 150030, PR China; Corresponding author.Glycosylated protein by transglutaminase-type could alter the structures and functional properties of proteins. However, the beneficial effects of glycosylated proteins against alcohol-induced intestinal injury remain unclear. Herein, the protective role and mechanism of glycosylated casein ameliorating alcohol-induced intestinal injury were assessed in vivo. The results showed that glycosylated casein and casein significantly relieved alcohol-induced oxidative stress and inflammatory responses in a concentration-dependent manner. The interventions of glycosylated casein and casein decreased serum parameters, improved the activities of antioxidant enzymes, reduced levels of inflammatory cytokines, and alleviated histopathological impairment in intestine tissues. Glycosylated casein and casein treatment downregulated protein expressions of TLR4, NF-κB p65, IKB-α, and MyD88 in mice, which activated the TLR4/NF-κB signaling pathway as a protective mechanism. More importantly, glycosylated caseinate showed a better effect against alcohol-induced intestinal injury than casein, suggesting that transglutaminase-mediated glycosylation of proteins is a useful technique to enforce protein biofunctions in the intestine.http://www.sciencedirect.com/science/article/pii/S1756464623004929CaseinGlycosylationIntestinal inflammationAnti-oxidationTLR4/NF-κB |
| spellingShingle | Yu Wang Yingying Yue Huimin Wang Yujun Jiang Jia Shi Oligochitosan glycosylation of casein by TGase: Protective effect and mechanism on alcohol-induced intestinal injury in C57BL/6 mice Journal of Functional Foods Casein Glycosylation Intestinal inflammation Anti-oxidation TLR4/NF-κB |
| title | Oligochitosan glycosylation of casein by TGase: Protective effect and mechanism on alcohol-induced intestinal injury in C57BL/6 mice |
| title_full | Oligochitosan glycosylation of casein by TGase: Protective effect and mechanism on alcohol-induced intestinal injury in C57BL/6 mice |
| title_fullStr | Oligochitosan glycosylation of casein by TGase: Protective effect and mechanism on alcohol-induced intestinal injury in C57BL/6 mice |
| title_full_unstemmed | Oligochitosan glycosylation of casein by TGase: Protective effect and mechanism on alcohol-induced intestinal injury in C57BL/6 mice |
| title_short | Oligochitosan glycosylation of casein by TGase: Protective effect and mechanism on alcohol-induced intestinal injury in C57BL/6 mice |
| title_sort | oligochitosan glycosylation of casein by tgase protective effect and mechanism on alcohol induced intestinal injury in c57bl 6 mice |
| topic | Casein Glycosylation Intestinal inflammation Anti-oxidation TLR4/NF-κB |
| url | http://www.sciencedirect.com/science/article/pii/S1756464623004929 |
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