Anti-rheumatoid activity of a hexane-insoluble fraction from Plantago major in female Wistar rats induced by Complete Freund's Adjuvant
Background and aim: Plantago major has long been used for medical purposes in Indonesia. However, reports on the anti-arthritic activities of P. major are limited. Experimental procedure: The anti-arthritic properties of an n-hexane-insoluble fraction of dichloromethane extracts of P. major (IPM) we...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-05-01
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| Series: | Journal of Traditional and Complementary Medicine |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2225411021000882 |
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| author | Asih Triastuti Dimas Adhi Pradana Didit Erwin Saputra Namira Lianika Hilmy Ramadhan Wicaksono Thessy Dewi Anisari Sitarina Widyarini |
| author_facet | Asih Triastuti Dimas Adhi Pradana Didit Erwin Saputra Namira Lianika Hilmy Ramadhan Wicaksono Thessy Dewi Anisari Sitarina Widyarini |
| author_sort | Asih Triastuti |
| collection | DOAJ |
| description | Background and aim: Plantago major has long been used for medical purposes in Indonesia. However, reports on the anti-arthritic activities of P. major are limited. Experimental procedure: The anti-arthritic properties of an n-hexane-insoluble fraction of dichloromethane extracts of P. major (IPM) were evaluated using Complete Freund's Adjuvant (CFA)-induced arthritis induced in female Wistar rat by CFA. Diclofenac was used as a positive control. The volume of paw oedema, white blood cell count, lymphocytes, neutrophils, expression of TNF-α and Interleukin-6 and the histopathological features of the joint tissues were assessed to characterise IPM activity. Results: The IPM extract at doses of 280 and 420 mg/kg BW and diclofenac inhibited paw oedema by 15.70 %, 15.94 % and 19.71 % respectively. IPM also reduced the incidence of arthritis and arthritic index. Unlike untreated rats, animals treated with IPM showed a significant decrease in the number of neutrophils and decreased expression of TNF-α and Interleukin-6. Histopathological examination showed a reduction in the number of inflammatory cells and hyperplasia of the synovium after IPM treatment. Conclusion: This study showed that P. major displays anti-rheumatoid arthritis activity. |
| first_indexed | 2024-04-14T00:34:59Z |
| format | Article |
| id | doaj.art-6c660ce223d349fbb6cdea79aef255e7 |
| institution | Directory Open Access Journal |
| issn | 2225-4110 |
| language | English |
| last_indexed | 2024-04-14T00:34:59Z |
| publishDate | 2022-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Traditional and Complementary Medicine |
| spelling | doaj.art-6c660ce223d349fbb6cdea79aef255e72022-12-22T02:22:24ZengElsevierJournal of Traditional and Complementary Medicine2225-41102022-05-01123219224Anti-rheumatoid activity of a hexane-insoluble fraction from Plantago major in female Wistar rats induced by Complete Freund's AdjuvantAsih Triastuti0Dimas Adhi Pradana1Didit Erwin Saputra2Namira Lianika3Hilmy Ramadhan Wicaksono4Thessy Dewi Anisari5Sitarina Widyarini6Department of Pharmacy, Universitas Islam Indonesia, Yogyakarta, Indonesia; Corresponding author.Department of Pharmacy, Universitas Islam Indonesia, Yogyakarta, IndonesiaDepartment of Pharmacy, Universitas Islam Indonesia, Yogyakarta, IndonesiaDepartment of Pharmacy, Universitas Islam Indonesia, Yogyakarta, IndonesiaDepartment of Pharmacy, Universitas Islam Indonesia, Yogyakarta, IndonesiaDepartment of Pharmacy, Universitas Islam Indonesia, Yogyakarta, IndonesiaDepartment of Pathology Faculty of Veterinary Medicine Universitas Gadjah Mada, IndonesiaBackground and aim: Plantago major has long been used for medical purposes in Indonesia. However, reports on the anti-arthritic activities of P. major are limited. Experimental procedure: The anti-arthritic properties of an n-hexane-insoluble fraction of dichloromethane extracts of P. major (IPM) were evaluated using Complete Freund's Adjuvant (CFA)-induced arthritis induced in female Wistar rat by CFA. Diclofenac was used as a positive control. The volume of paw oedema, white blood cell count, lymphocytes, neutrophils, expression of TNF-α and Interleukin-6 and the histopathological features of the joint tissues were assessed to characterise IPM activity. Results: The IPM extract at doses of 280 and 420 mg/kg BW and diclofenac inhibited paw oedema by 15.70 %, 15.94 % and 19.71 % respectively. IPM also reduced the incidence of arthritis and arthritic index. Unlike untreated rats, animals treated with IPM showed a significant decrease in the number of neutrophils and decreased expression of TNF-α and Interleukin-6. Histopathological examination showed a reduction in the number of inflammatory cells and hyperplasia of the synovium after IPM treatment. Conclusion: This study showed that P. major displays anti-rheumatoid arthritis activity.http://www.sciencedirect.com/science/article/pii/S2225411021000882ArthritisHistopathologyCytokinesPaw oedema |
| spellingShingle | Asih Triastuti Dimas Adhi Pradana Didit Erwin Saputra Namira Lianika Hilmy Ramadhan Wicaksono Thessy Dewi Anisari Sitarina Widyarini Anti-rheumatoid activity of a hexane-insoluble fraction from Plantago major in female Wistar rats induced by Complete Freund's Adjuvant Journal of Traditional and Complementary Medicine Arthritis Histopathology Cytokines Paw oedema |
| title | Anti-rheumatoid activity of a hexane-insoluble fraction from Plantago major in female Wistar rats induced by Complete Freund's Adjuvant |
| title_full | Anti-rheumatoid activity of a hexane-insoluble fraction from Plantago major in female Wistar rats induced by Complete Freund's Adjuvant |
| title_fullStr | Anti-rheumatoid activity of a hexane-insoluble fraction from Plantago major in female Wistar rats induced by Complete Freund's Adjuvant |
| title_full_unstemmed | Anti-rheumatoid activity of a hexane-insoluble fraction from Plantago major in female Wistar rats induced by Complete Freund's Adjuvant |
| title_short | Anti-rheumatoid activity of a hexane-insoluble fraction from Plantago major in female Wistar rats induced by Complete Freund's Adjuvant |
| title_sort | anti rheumatoid activity of a hexane insoluble fraction from plantago major in female wistar rats induced by complete freund s adjuvant |
| topic | Arthritis Histopathology Cytokines Paw oedema |
| url | http://www.sciencedirect.com/science/article/pii/S2225411021000882 |
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