Magnetite-Based Nanostructured Coatings Functionalized with <i>Nigella sativa</i> and Dicloxacillin for Improved Wound Dressings
In this study, we report the performance improvement of wound dressings by covering them with magnetite-based nanostructured coatings. The magnetite nanoparticles (Fe<sub>3</sub>O<sub>4</sub> NPs) were functionalized with <i>Nigella sativa</i> (<i>N. sativa&...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/12/1/59 |
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| author | Gabriela Dorcioman Ariana Hudiță Bianca Gălățeanu Doina Craciun Ionel Mercioniu Ovidiu Cristian Oprea Irina Neguț Valentina Grumezescu Alexandru Mihai Grumezescu Lia Mara Dițu Alina Maria Holban |
| author_facet | Gabriela Dorcioman Ariana Hudiță Bianca Gălățeanu Doina Craciun Ionel Mercioniu Ovidiu Cristian Oprea Irina Neguț Valentina Grumezescu Alexandru Mihai Grumezescu Lia Mara Dițu Alina Maria Holban |
| author_sort | Gabriela Dorcioman |
| collection | DOAJ |
| description | In this study, we report the performance improvement of wound dressings by covering them with magnetite-based nanostructured coatings. The magnetite nanoparticles (Fe<sub>3</sub>O<sub>4</sub> NPs) were functionalized with <i>Nigella sativa</i> (<i>N. sativa</i>) powder/essential oil and dicloxacillin and were synthesized as coatings by matrix assisted pulsed laser evaporation (MAPLE). The expected effects of this combination of materials are: (i) to reduce microbial contamination, and (ii) to promote rapid wound healing. The crystalline nature of <i>core/shell</i> Fe<sub>3</sub>O<sub>4</sub> NPs and coatings was determined by X-ray diffraction (XRD). Differential Scanning Calorimetry (DSC) and Thermo Gravimetric Analysis (TGA) have been coupled to investigate the stability and thermal degradation of <i>core/shell</i> nanoparticle components. The coatings’ morphology was examined by scanning electron microscopy (SEM). The distribution of chemical elements and functional groups in the resulting coatings was evidenced by Fourier transform infrared (FTIR) spectrometry. In order to simulate the interaction between wound dressings and epithelial tissues and to evaluate the drug release in time, the samples were immersed in simulated body fluid (SBF) and investigated after different durations of time. The antimicrobial effect was evaluated in planktonic (free-floating) and attached (biofilms) bacteria models. The biocompatibility and regenerative properties of the nanostructured coatings were evaluated <i>in vitro</i>, at cellular, biochemical, and the molecular level. The obtained results show that magnetite-based nanostructured coatings functionalized with <i>N. sativa</i> and dicloxacillin are biocompatible and show an enhanced antimicrobial effect against Gram positive and Gram negative opportunistic bacteria. |
| first_indexed | 2024-03-09T13:48:10Z |
| format | Article |
| id | doaj.art-6c66e1390d1d4b4ba9004d42be9c78cd |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-09T13:48:10Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-6c66e1390d1d4b4ba9004d42be9c78cd2023-11-30T20:55:08ZengMDPI AGAntibiotics2079-63822022-12-011215910.3390/antibiotics12010059Magnetite-Based Nanostructured Coatings Functionalized with <i>Nigella sativa</i> and Dicloxacillin for Improved Wound DressingsGabriela Dorcioman0Ariana Hudiță1Bianca Gălățeanu2Doina Craciun3Ionel Mercioniu4Ovidiu Cristian Oprea5Irina Neguț6Valentina Grumezescu7Alexandru Mihai Grumezescu8Lia Mara Dițu9Alina Maria Holban10Lasers Department, National Institute for Lasers, Plasma and Radiation Physics, 409 Atomistilor Street, 077125 Magurele, RomaniaDepartment of Biochemistry and Molecular Biology, University of Bucharest, 050095 Bucharest, RomaniaDepartment of Biochemistry and Molecular Biology, University of Bucharest, 050095 Bucharest, RomaniaLasers Department, National Institute for Lasers, Plasma and Radiation Physics, 409 Atomistilor Street, 077125 Magurele, RomaniaNational Institute of Materials Physics, 405A Atomistilor Street, 077125 Magurele, RomaniaDepartment of Inorganic Chemistry, Physical Chemistry and Electrochemistry, Faculty of Applied Chemistry and Materials Science, Politehnica University of Bucharest, 1-7 Gheorghe Polizu Street, 011061 Bucharest, RomaniaLasers Department, National Institute for Lasers, Plasma and Radiation Physics, 409 Atomistilor Street, 077125 Magurele, RomaniaLasers Department, National Institute for Lasers, Plasma and Radiation Physics, 409 Atomistilor Street, 077125 Magurele, RomaniaAcademy of Romanian Scientists, Ilfov No. 3, 050044 Bucharest, RomaniaDepartment of Microbiology and Immunology, Faculty of Biology, University of Bucharest, 91-95 Splaiul Independentei Street, 077206 Bucharest, RomaniaDepartment of Microbiology and Immunology, Faculty of Biology, University of Bucharest, 91-95 Splaiul Independentei Street, 077206 Bucharest, RomaniaIn this study, we report the performance improvement of wound dressings by covering them with magnetite-based nanostructured coatings. The magnetite nanoparticles (Fe<sub>3</sub>O<sub>4</sub> NPs) were functionalized with <i>Nigella sativa</i> (<i>N. sativa</i>) powder/essential oil and dicloxacillin and were synthesized as coatings by matrix assisted pulsed laser evaporation (MAPLE). The expected effects of this combination of materials are: (i) to reduce microbial contamination, and (ii) to promote rapid wound healing. The crystalline nature of <i>core/shell</i> Fe<sub>3</sub>O<sub>4</sub> NPs and coatings was determined by X-ray diffraction (XRD). Differential Scanning Calorimetry (DSC) and Thermo Gravimetric Analysis (TGA) have been coupled to investigate the stability and thermal degradation of <i>core/shell</i> nanoparticle components. The coatings’ morphology was examined by scanning electron microscopy (SEM). The distribution of chemical elements and functional groups in the resulting coatings was evidenced by Fourier transform infrared (FTIR) spectrometry. In order to simulate the interaction between wound dressings and epithelial tissues and to evaluate the drug release in time, the samples were immersed in simulated body fluid (SBF) and investigated after different durations of time. The antimicrobial effect was evaluated in planktonic (free-floating) and attached (biofilms) bacteria models. The biocompatibility and regenerative properties of the nanostructured coatings were evaluated <i>in vitro</i>, at cellular, biochemical, and the molecular level. The obtained results show that magnetite-based nanostructured coatings functionalized with <i>N. sativa</i> and dicloxacillin are biocompatible and show an enhanced antimicrobial effect against Gram positive and Gram negative opportunistic bacteria.https://www.mdpi.com/2079-6382/12/1/59magnetite nanoparticlescoatingsdrug releaseantimicrobial potentialwound dressings |
| spellingShingle | Gabriela Dorcioman Ariana Hudiță Bianca Gălățeanu Doina Craciun Ionel Mercioniu Ovidiu Cristian Oprea Irina Neguț Valentina Grumezescu Alexandru Mihai Grumezescu Lia Mara Dițu Alina Maria Holban Magnetite-Based Nanostructured Coatings Functionalized with <i>Nigella sativa</i> and Dicloxacillin for Improved Wound Dressings Antibiotics magnetite nanoparticles coatings drug release antimicrobial potential wound dressings |
| title | Magnetite-Based Nanostructured Coatings Functionalized with <i>Nigella sativa</i> and Dicloxacillin for Improved Wound Dressings |
| title_full | Magnetite-Based Nanostructured Coatings Functionalized with <i>Nigella sativa</i> and Dicloxacillin for Improved Wound Dressings |
| title_fullStr | Magnetite-Based Nanostructured Coatings Functionalized with <i>Nigella sativa</i> and Dicloxacillin for Improved Wound Dressings |
| title_full_unstemmed | Magnetite-Based Nanostructured Coatings Functionalized with <i>Nigella sativa</i> and Dicloxacillin for Improved Wound Dressings |
| title_short | Magnetite-Based Nanostructured Coatings Functionalized with <i>Nigella sativa</i> and Dicloxacillin for Improved Wound Dressings |
| title_sort | magnetite based nanostructured coatings functionalized with i nigella sativa i and dicloxacillin for improved wound dressings |
| topic | magnetite nanoparticles coatings drug release antimicrobial potential wound dressings |
| url | https://www.mdpi.com/2079-6382/12/1/59 |
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