The effect of ATP-dependent K(+)-channel opener on transmembrane potassium exchange and reactive oxygen species production upon the opening of mitochondrial pore
The effect of mitochondrial ATP-dependent K+-channel (K+ATP-channel) opener diazoxide (DZ) on transmembrane potassium exchange and reactive oxygen species (ROS) formation under the opening of mitochondrial permeability transition pore (MPTP) was studied in rat liver mitochondria. The activation of K...
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| Format: | Article |
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2014-04-01
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| Series: | The Ukrainian Biochemical Journal |
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| Online Access: | http://ukrbiochemjournal.org/wp-content/uploads/2015/06/Akopova_2_14.pdf |
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| author | O. V. Akopova L. I. Kolchinskaya V. I. Nosar V. A. Bouryi I. N. Mankovska V. F. Sagach |
| author_facet | O. V. Akopova L. I. Kolchinskaya V. I. Nosar V. A. Bouryi I. N. Mankovska V. F. Sagach |
| author_sort | O. V. Akopova |
| collection | DOAJ |
| description | The effect of mitochondrial ATP-dependent K+-channel (K+ATP-channel) opener diazoxide (DZ) on transmembrane potassium exchange and reactive oxygen species (ROS) formation under the opening of mitochondrial permeability transition pore (MPTP) was studied in rat liver mitochondria. The activation of K+-cycling (K+-uptake and K+/H+-exchange) by DZ was established with peak effect at ≤500 nM. It was shown that MPTP opening as well resulted in the activation of K+-cycling together with simultaneous activation of Ca2+-cycle in mitochondria. In the absence of depolarization Ca2+-cycle is supported by MPTP and Ca2+-uniporter. The stimulation of K+/H+-exchange by MPTP opening led to the activation of K+-cycle, but further activation of K+/H+-exchange resulted in MPTP inhibition. Under the same conditions the decrease in mitochondrial ROS production was observed. It was proposed that the decrease in ROS formation together with K+/Н+-exchange activation could be the constituents of the complex effect of MPTP inhibition induced by K+ATP-channel opener. |
| first_indexed | 2024-03-09T07:30:16Z |
| format | Article |
| id | doaj.art-6c673ef62ae04fcd8cde3603d8889862 |
| institution | Directory Open Access Journal |
| issn | 2409-4943 2413-5003 |
| language | English |
| last_indexed | 2024-03-09T07:30:16Z |
| publishDate | 2014-04-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | The Ukrainian Biochemical Journal |
| spelling | doaj.art-6c673ef62ae04fcd8cde3603d88898622023-12-03T06:26:48ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryThe Ukrainian Biochemical Journal2409-49432413-50032014-04-01862264010.15407/ubj86.02.026The effect of ATP-dependent K(+)-channel opener on transmembrane potassium exchange and reactive oxygen species production upon the opening of mitochondrial poreO. V. Akopova0L. I. Kolchinskaya1V. I. Nosar2V. A. Bouryi3I. N. Mankovska4V. F. Sagach5Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, KyivBogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, KyivBogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, KyivBogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, KyivBogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, KyivBogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, KyivThe effect of mitochondrial ATP-dependent K+-channel (K+ATP-channel) opener diazoxide (DZ) on transmembrane potassium exchange and reactive oxygen species (ROS) formation under the opening of mitochondrial permeability transition pore (MPTP) was studied in rat liver mitochondria. The activation of K+-cycling (K+-uptake and K+/H+-exchange) by DZ was established with peak effect at ≤500 nM. It was shown that MPTP opening as well resulted in the activation of K+-cycling together with simultaneous activation of Ca2+-cycle in mitochondria. In the absence of depolarization Ca2+-cycle is supported by MPTP and Ca2+-uniporter. The stimulation of K+/H+-exchange by MPTP opening led to the activation of K+-cycle, but further activation of K+/H+-exchange resulted in MPTP inhibition. Under the same conditions the decrease in mitochondrial ROS production was observed. It was proposed that the decrease in ROS formation together with K+/Н+-exchange activation could be the constituents of the complex effect of MPTP inhibition induced by K+ATP-channel opener.http://ukrbiochemjournal.org/wp-content/uploads/2015/06/Akopova_2_14.pdfCa(2+)-cycleK(+)/H(+)-exchangeK+ATP-channelliver mitochondria ratsmitochondrial porereactive oxygen species |
| spellingShingle | O. V. Akopova L. I. Kolchinskaya V. I. Nosar V. A. Bouryi I. N. Mankovska V. F. Sagach The effect of ATP-dependent K(+)-channel opener on transmembrane potassium exchange and reactive oxygen species production upon the opening of mitochondrial pore The Ukrainian Biochemical Journal Ca(2+)-cycle K(+)/H(+)-exchange K+ATP-channel liver mitochondria rats mitochondrial pore reactive oxygen species |
| title | The effect of ATP-dependent K(+)-channel opener on transmembrane potassium exchange and reactive oxygen species production upon the opening of mitochondrial pore |
| title_full | The effect of ATP-dependent K(+)-channel opener on transmembrane potassium exchange and reactive oxygen species production upon the opening of mitochondrial pore |
| title_fullStr | The effect of ATP-dependent K(+)-channel opener on transmembrane potassium exchange and reactive oxygen species production upon the opening of mitochondrial pore |
| title_full_unstemmed | The effect of ATP-dependent K(+)-channel opener on transmembrane potassium exchange and reactive oxygen species production upon the opening of mitochondrial pore |
| title_short | The effect of ATP-dependent K(+)-channel opener on transmembrane potassium exchange and reactive oxygen species production upon the opening of mitochondrial pore |
| title_sort | effect of atp dependent k channel opener on transmembrane potassium exchange and reactive oxygen species production upon the opening of mitochondrial pore |
| topic | Ca(2+)-cycle K(+)/H(+)-exchange K+ATP-channel liver mitochondria rats mitochondrial pore reactive oxygen species |
| url | http://ukrbiochemjournal.org/wp-content/uploads/2015/06/Akopova_2_14.pdf |
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