Traditional mineral medicine realgar and Realgar-Indigo naturalis formula potentially exerted therapeutic effects by altering the gut microbiota
IntroductionRealgar has a long history ofuse in traditional medicines. However, the mechanism through which Realgar or Realgar-Indigo naturalis formula (RIF) exert therapeutic effects is only partially understood.MethodsIn this study, 60 feces and 60 ileum samples from rats administered with realgar...
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Frontiers Media S.A.
2023-04-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1143173/full |
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| author | Lu Luo Lu Luo Chaofeng Li Chaofeng Li Nanxi Huang Qiaochu Wang Zihao Zhang Chen Song Hui Yang Maowen Yuan Ziwen Xu Jialei Sun Zhijie Zhang |
| author_facet | Lu Luo Lu Luo Chaofeng Li Chaofeng Li Nanxi Huang Qiaochu Wang Zihao Zhang Chen Song Hui Yang Maowen Yuan Ziwen Xu Jialei Sun Zhijie Zhang |
| author_sort | Lu Luo |
| collection | DOAJ |
| description | IntroductionRealgar has a long history ofuse in traditional medicines. However, the mechanism through which Realgar or Realgar-Indigo naturalis formula (RIF) exert therapeutic effects is only partially understood.MethodsIn this study, 60 feces and 60 ileum samples from rats administered with realgar or RIF were collected to examine the gut microbiota.ResultsThe results showed that realgar and RIF influenced different microbiota in both feces and ileum. Compared with realgar, RIF at low dosage (0.1701 g/3 ml) significantly increased the microbiota diversity. LEfSe and random forest analyses showed that the bacterium Bacteroidales was significantly altered after RIF administration, and it was predicted that these microorganisms contribute to the inorganic arsenic metabolic process.DiscussionOur results suggest that realgar and RIF may exert their therapeutic effects through influencing microbiota. The low dose of RIF had greater effects on increasing the diversity of microbiota, and Bacteroidales in feces might participate in the inorganic arsenic metabolic process to exert therapeutic effects for realgar. |
| first_indexed | 2024-04-09T17:31:26Z |
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| id | doaj.art-6c6cb9dbfc9944378a8c030b7d1cf659 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-09T17:31:26Z |
| publishDate | 2023-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-6c6cb9dbfc9944378a8c030b7d1cf6592023-04-18T05:03:45ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-04-011410.3389/fmicb.2023.11431731143173Traditional mineral medicine realgar and Realgar-Indigo naturalis formula potentially exerted therapeutic effects by altering the gut microbiotaLu Luo0Lu Luo1Chaofeng Li2Chaofeng Li3Nanxi Huang4Qiaochu Wang5Zihao Zhang6Chen Song7Hui Yang8Maowen Yuan9Ziwen Xu10Jialei Sun11Zhijie Zhang12Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, ChinaDepartment of Biochemistry and Molecular & Cellular Biology, Georgetown University Medical Center, Washington, DC, United StatesInstitute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, ChinaSchool of Pharmaceutical Sciences, Tsinghua University, Beijing, ChinaDepartment of Biochemistry and Molecular & Cellular Biology, Georgetown University Medical Center, Washington, DC, United StatesDepartment of Biochemistry and Molecular & Cellular Biology, Georgetown University Medical Center, Washington, DC, United StatesDepartment of Biochemistry and Molecular & Cellular Biology, Georgetown University Medical Center, Washington, DC, United StatesInstitute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, ChinaInstitute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, ChinaSchool of Earth Science and Resources, China University of Geosciences, Beijing, ChinaDepartment of Biochemistry and Molecular & Cellular Biology, Georgetown University Medical Center, Washington, DC, United StatesThird Affiliated Hospital of Zhejiang University of Traditional Chinese Medicine, Hangzhou, ChinaInstitute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, ChinaIntroductionRealgar has a long history ofuse in traditional medicines. However, the mechanism through which Realgar or Realgar-Indigo naturalis formula (RIF) exert therapeutic effects is only partially understood.MethodsIn this study, 60 feces and 60 ileum samples from rats administered with realgar or RIF were collected to examine the gut microbiota.ResultsThe results showed that realgar and RIF influenced different microbiota in both feces and ileum. Compared with realgar, RIF at low dosage (0.1701 g/3 ml) significantly increased the microbiota diversity. LEfSe and random forest analyses showed that the bacterium Bacteroidales was significantly altered after RIF administration, and it was predicted that these microorganisms contribute to the inorganic arsenic metabolic process.DiscussionOur results suggest that realgar and RIF may exert their therapeutic effects through influencing microbiota. The low dose of RIF had greater effects on increasing the diversity of microbiota, and Bacteroidales in feces might participate in the inorganic arsenic metabolic process to exert therapeutic effects for realgar.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1143173/fullrealgarRealgar-Indigo naturalis formula (RIF)gut microbiotaBacteroidalesmineral medicine |
| spellingShingle | Lu Luo Lu Luo Chaofeng Li Chaofeng Li Nanxi Huang Qiaochu Wang Zihao Zhang Chen Song Hui Yang Maowen Yuan Ziwen Xu Jialei Sun Zhijie Zhang Traditional mineral medicine realgar and Realgar-Indigo naturalis formula potentially exerted therapeutic effects by altering the gut microbiota Frontiers in Microbiology realgar Realgar-Indigo naturalis formula (RIF) gut microbiota Bacteroidales mineral medicine |
| title | Traditional mineral medicine realgar and Realgar-Indigo naturalis formula potentially exerted therapeutic effects by altering the gut microbiota |
| title_full | Traditional mineral medicine realgar and Realgar-Indigo naturalis formula potentially exerted therapeutic effects by altering the gut microbiota |
| title_fullStr | Traditional mineral medicine realgar and Realgar-Indigo naturalis formula potentially exerted therapeutic effects by altering the gut microbiota |
| title_full_unstemmed | Traditional mineral medicine realgar and Realgar-Indigo naturalis formula potentially exerted therapeutic effects by altering the gut microbiota |
| title_short | Traditional mineral medicine realgar and Realgar-Indigo naturalis formula potentially exerted therapeutic effects by altering the gut microbiota |
| title_sort | traditional mineral medicine realgar and realgar indigo naturalis formula potentially exerted therapeutic effects by altering the gut microbiota |
| topic | realgar Realgar-Indigo naturalis formula (RIF) gut microbiota Bacteroidales mineral medicine |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1143173/full |
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