UPLC-MS/MS Profile Combined With RNA-Seq Reveals the Amino Acid Metabolism in Zanthoxylum bungeanum Leaves Under Drought Stress
Zanthoxylum bungeanum leaves have a unique taste and incomparable nutritional value and hence are popular as a food item and traditional medicine in China. However, the studies on the metabolites in Z. bungeanum leaves are quite limited, especially for amino acids. Therefore, this study explored the...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2022.921742/full |
| _version_ | 1818159286052716544 |
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| author | Haichao Hu Haichao Hu Xitong Fei Xitong Fei Beibei He Xin Chen Xin Chen Lei Ma Lei Ma Peilin Han Peilin Han Yingli Luo Yingli Luo Yonghong Liu Yonghong Liu Anzhi Wei Anzhi Wei |
| author_facet | Haichao Hu Haichao Hu Xitong Fei Xitong Fei Beibei He Xin Chen Xin Chen Lei Ma Lei Ma Peilin Han Peilin Han Yingli Luo Yingli Luo Yonghong Liu Yonghong Liu Anzhi Wei Anzhi Wei |
| author_sort | Haichao Hu |
| collection | DOAJ |
| description | Zanthoxylum bungeanum leaves have a unique taste and incomparable nutritional value and hence are popular as a food item and traditional medicine in China. However, the studies on the metabolites in Z. bungeanum leaves are quite limited, especially for amino acids. Therefore, this study explored the amino acid component in Z. bungeanum leaves and also the accumulation mechanism under drought stress in two Z. bungeanum cultivars using the widely targeted metabolome combined with transcriptome analysis. A total of 56 amino acids and their derivatives were identified in Z. bungeanum leaves, including eight essential amino acids. The total amino acid content with most individual amino acids increased under progressive drought stress. More differentially accumulated amino acids (DAAs) and differentially expressed genes (DEGs) were found in FJ (Z. bungeanum cv. ‘Fengjiao’) than in HJ (Z. bungeanum cv. ‘Hanjiao’). The orthogonal projections to latent structures discriminant analysis identified nine and seven indicator DAAs in FJ and HJ leaves, respectively. The weighted gene co-expression network analysis (WGCNA) showed that the green module was significantly correlated with most indicator DAAs and revealed the important role of FBA3, DELTA-OAT, PROC, and 15 transcription factor genes in regulating the amino acid synthesis. Furthermore, the correlation analysis and redundancy analysis (RDA) identified four candidate synthesis genes (ASNS, AK, ASPS, and PK) in amino acid biosynthesis pathway. This study provided useful information for the development of Z. bungeanum leaves in food and nutrition industry and also laid the foundations for future molecular breeding. |
| first_indexed | 2024-12-11T15:43:34Z |
| format | Article |
| id | doaj.art-6c6cd6b788b64314bf8b2b6a0ffd04e1 |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-12-11T15:43:34Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-6c6cd6b788b64314bf8b2b6a0ffd04e12022-12-22T00:59:45ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2022-07-01910.3389/fnut.2022.921742921742UPLC-MS/MS Profile Combined With RNA-Seq Reveals the Amino Acid Metabolism in Zanthoxylum bungeanum Leaves Under Drought StressHaichao Hu0Haichao Hu1Xitong Fei2Xitong Fei3Beibei He4Xin Chen5Xin Chen6Lei Ma7Lei Ma8Peilin Han9Peilin Han10Yingli Luo11Yingli Luo12Yonghong Liu13Yonghong Liu14Anzhi Wei15Anzhi Wei16College of Forestry, Northwest Agriculture and Forestry University, Xianyang, ChinaResearch Centre for Engineering and Technology of Zanthoxylum State Forestry Administration, Xianyang, ChinaCollege of Forestry, Northwest Agriculture and Forestry University, Xianyang, ChinaResearch Centre for Engineering and Technology of Zanthoxylum State Forestry Administration, Xianyang, ChinaCollege of Horticulture, Northwest Agriculture and Forestry University, Xianyang, ChinaCollege of Forestry, Northwest Agriculture and Forestry University, Xianyang, ChinaResearch Centre for Engineering and Technology of Zanthoxylum State Forestry Administration, Xianyang, ChinaCollege of Forestry, Northwest Agriculture and Forestry University, Xianyang, ChinaResearch Centre for Engineering and Technology of Zanthoxylum State Forestry Administration, Xianyang, ChinaCollege of Forestry, Northwest Agriculture and Forestry University, Xianyang, ChinaResearch Centre for Engineering and Technology of Zanthoxylum State Forestry Administration, Xianyang, ChinaCollege of Forestry, Northwest Agriculture and Forestry University, Xianyang, ChinaResearch Centre for Engineering and Technology of Zanthoxylum State Forestry Administration, Xianyang, ChinaCollege of Forestry, Northwest Agriculture and Forestry University, Xianyang, ChinaResearch Centre for Engineering and Technology of Zanthoxylum State Forestry Administration, Xianyang, ChinaCollege of Forestry, Northwest Agriculture and Forestry University, Xianyang, ChinaResearch Centre for Engineering and Technology of Zanthoxylum State Forestry Administration, Xianyang, ChinaZanthoxylum bungeanum leaves have a unique taste and incomparable nutritional value and hence are popular as a food item and traditional medicine in China. However, the studies on the metabolites in Z. bungeanum leaves are quite limited, especially for amino acids. Therefore, this study explored the amino acid component in Z. bungeanum leaves and also the accumulation mechanism under drought stress in two Z. bungeanum cultivars using the widely targeted metabolome combined with transcriptome analysis. A total of 56 amino acids and their derivatives were identified in Z. bungeanum leaves, including eight essential amino acids. The total amino acid content with most individual amino acids increased under progressive drought stress. More differentially accumulated amino acids (DAAs) and differentially expressed genes (DEGs) were found in FJ (Z. bungeanum cv. ‘Fengjiao’) than in HJ (Z. bungeanum cv. ‘Hanjiao’). The orthogonal projections to latent structures discriminant analysis identified nine and seven indicator DAAs in FJ and HJ leaves, respectively. The weighted gene co-expression network analysis (WGCNA) showed that the green module was significantly correlated with most indicator DAAs and revealed the important role of FBA3, DELTA-OAT, PROC, and 15 transcription factor genes in regulating the amino acid synthesis. Furthermore, the correlation analysis and redundancy analysis (RDA) identified four candidate synthesis genes (ASNS, AK, ASPS, and PK) in amino acid biosynthesis pathway. This study provided useful information for the development of Z. bungeanum leaves in food and nutrition industry and also laid the foundations for future molecular breeding.https://www.frontiersin.org/articles/10.3389/fnut.2022.921742/fullZanthoxylum bungeanum leavesamino acidmetabolometranscriptomeredundancy analysis |
| spellingShingle | Haichao Hu Haichao Hu Xitong Fei Xitong Fei Beibei He Xin Chen Xin Chen Lei Ma Lei Ma Peilin Han Peilin Han Yingli Luo Yingli Luo Yonghong Liu Yonghong Liu Anzhi Wei Anzhi Wei UPLC-MS/MS Profile Combined With RNA-Seq Reveals the Amino Acid Metabolism in Zanthoxylum bungeanum Leaves Under Drought Stress Frontiers in Nutrition Zanthoxylum bungeanum leaves amino acid metabolome transcriptome redundancy analysis |
| title | UPLC-MS/MS Profile Combined With RNA-Seq Reveals the Amino Acid Metabolism in Zanthoxylum bungeanum Leaves Under Drought Stress |
| title_full | UPLC-MS/MS Profile Combined With RNA-Seq Reveals the Amino Acid Metabolism in Zanthoxylum bungeanum Leaves Under Drought Stress |
| title_fullStr | UPLC-MS/MS Profile Combined With RNA-Seq Reveals the Amino Acid Metabolism in Zanthoxylum bungeanum Leaves Under Drought Stress |
| title_full_unstemmed | UPLC-MS/MS Profile Combined With RNA-Seq Reveals the Amino Acid Metabolism in Zanthoxylum bungeanum Leaves Under Drought Stress |
| title_short | UPLC-MS/MS Profile Combined With RNA-Seq Reveals the Amino Acid Metabolism in Zanthoxylum bungeanum Leaves Under Drought Stress |
| title_sort | uplc ms ms profile combined with rna seq reveals the amino acid metabolism in zanthoxylum bungeanum leaves under drought stress |
| topic | Zanthoxylum bungeanum leaves amino acid metabolome transcriptome redundancy analysis |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2022.921742/full |
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