Experimental ventilator-associated pneumonia: distribution of lung infection and consequences for lung aeration
Ventilator-associated pneumonia (VAP) has been described in humans and in experimental animals. The most severe lesions are located in dependent lung segments along a sterno-vertebral axis, however the cephalocaudal distribution of lung infection remains unknown. We used an experimental model to eva...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2003-01-01
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| Series: | Brazilian Journal of Infectious Diseases |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702003000300007 |
| _version_ | 1819295280500047872 |
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| author | Vieira Silvia Regina Rios Goldstein Ivan Lenaour Gilles Marquette Charles-Hugo Rouby Jean-Jacques |
| author_facet | Vieira Silvia Regina Rios Goldstein Ivan Lenaour Gilles Marquette Charles-Hugo Rouby Jean-Jacques |
| author_sort | Vieira Silvia Regina Rios |
| collection | DOAJ |
| description | Ventilator-associated pneumonia (VAP) has been described in humans and in experimental animals. The most severe lesions are located in dependent lung segments along a sterno-vertebral axis, however the cephalocaudal distribution of lung infection remains unknown. We used an experimental model to evaluate the distribution of lung infection, considering its anteroposterior and cephalocaudal gradient, and its impact on lung aeration. Ten healthy domestic piglets were anesthetized, paralyzed and mechanically ventilated for 59 hours in the prone position. At the end of the experiment they were sacrificed and their lungs were fixed. Six segments were analyzed: a non-dependant (ND) and a dependant (D) segment of the upper (UL), middle (ML) and lower (LL) lobes. The presence of healthy lung or of histological infectious lesions was analyzed with a semi-quantitative method. The regional distribution of lung infection was compared between upper, middle and lower lobes, as well as between dependant and non-dependant regions. The presence of infectious lesions was correlated with measurements of lung aeration. Nine of the ten piglets developed VAP. Infectious lesions were distributed along a sterno-vertebral and a cephalocaudal gradient; the lower and middle lobes were more frequently infected than the upper lobes. There was an inverse correlation (R= - 0.902) between the development of lung lesions and lung aeration. In conclusion, VAP was a frequent complication in healthy mechanically ventilated piglets, showing an anteroposterior as well as a cephalocaudal gradient. As expected, development of lung infection was accompanied by a corresponding loss of aeration. |
| first_indexed | 2024-12-24T04:39:42Z |
| format | Article |
| id | doaj.art-6c6dd6b419b842fea8b389eccce8ec3d |
| institution | Directory Open Access Journal |
| issn | 1413-8670 1678-4391 |
| language | English |
| last_indexed | 2024-12-24T04:39:42Z |
| publishDate | 2003-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Brazilian Journal of Infectious Diseases |
| spelling | doaj.art-6c6dd6b419b842fea8b389eccce8ec3d2022-12-21T17:14:53ZengElsevierBrazilian Journal of Infectious Diseases1413-86701678-43912003-01-0173216223Experimental ventilator-associated pneumonia: distribution of lung infection and consequences for lung aerationVieira Silvia Regina RiosGoldstein IvanLenaour GillesMarquette Charles-HugoRouby Jean-JacquesVentilator-associated pneumonia (VAP) has been described in humans and in experimental animals. The most severe lesions are located in dependent lung segments along a sterno-vertebral axis, however the cephalocaudal distribution of lung infection remains unknown. We used an experimental model to evaluate the distribution of lung infection, considering its anteroposterior and cephalocaudal gradient, and its impact on lung aeration. Ten healthy domestic piglets were anesthetized, paralyzed and mechanically ventilated for 59 hours in the prone position. At the end of the experiment they were sacrificed and their lungs were fixed. Six segments were analyzed: a non-dependant (ND) and a dependant (D) segment of the upper (UL), middle (ML) and lower (LL) lobes. The presence of healthy lung or of histological infectious lesions was analyzed with a semi-quantitative method. The regional distribution of lung infection was compared between upper, middle and lower lobes, as well as between dependant and non-dependant regions. The presence of infectious lesions was correlated with measurements of lung aeration. Nine of the ten piglets developed VAP. Infectious lesions were distributed along a sterno-vertebral and a cephalocaudal gradient; the lower and middle lobes were more frequently infected than the upper lobes. There was an inverse correlation (R= - 0.902) between the development of lung lesions and lung aeration. In conclusion, VAP was a frequent complication in healthy mechanically ventilated piglets, showing an anteroposterior as well as a cephalocaudal gradient. As expected, development of lung infection was accompanied by a corresponding loss of aeration.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702003000300007Ventilator associated pneumonia |
| spellingShingle | Vieira Silvia Regina Rios Goldstein Ivan Lenaour Gilles Marquette Charles-Hugo Rouby Jean-Jacques Experimental ventilator-associated pneumonia: distribution of lung infection and consequences for lung aeration Brazilian Journal of Infectious Diseases Ventilator associated pneumonia |
| title | Experimental ventilator-associated pneumonia: distribution of lung infection and consequences for lung aeration |
| title_full | Experimental ventilator-associated pneumonia: distribution of lung infection and consequences for lung aeration |
| title_fullStr | Experimental ventilator-associated pneumonia: distribution of lung infection and consequences for lung aeration |
| title_full_unstemmed | Experimental ventilator-associated pneumonia: distribution of lung infection and consequences for lung aeration |
| title_short | Experimental ventilator-associated pneumonia: distribution of lung infection and consequences for lung aeration |
| title_sort | experimental ventilator associated pneumonia distribution of lung infection and consequences for lung aeration |
| topic | Ventilator associated pneumonia |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702003000300007 |
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